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![Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: FFPE. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:500 for 4 degrees Celsius for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type III is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-NB600-594-img0018.jpg "Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: FFPE. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:500 for 4 degrees Celsius for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type III is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.")
![Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Lane 1: Human Collagen III Load: 100 ng per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4C Secondary antibody: DyLight 649 Goat anti-rabbit at 1:20,000 for 30 min at RT Block: incubated with blocking buffer for 30 min at RT Predicted/Observed size: 138 kDa/138 kDa.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Western-Blot-NB600-594-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human primary ventricular cardiac fibroblasts were stained with anti-Collagen III antibody. Cells were cultured for 3 days in DMEM with 10% fetal calf serum. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-594-img0012.jpg)
![Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C. 20 minutes for antigen retrieval.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-NB600-594-img0016.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Immunodetection of ID3, COL1A2 and COL3A1 in human dermal fibroblasts subjected to treatment with or without cANGPTL4 (12 ug/mL) in the presence of TGFB1 (10 ng/mL). B-tubulin, as loading control, was from the same samples. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-05869-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Western-Blot-NB600-594-img0023.jpg)
![Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Livers were harvested at 9 wpi, formalin-fixed, and then cryosectioned and stained for markers of inflammation and liver fibrosis. Maximum intensity projections of 12-17 um thick liver sections with immunofluorescently labeled nuclei (DAPI white) alpha-smooth muscle actin NBP2-34760APC (yellow), CD45 (red) and Collagen I NB600-408 (top) or Collagen III/COL3A1 NB600-594 (bottom) (blue) in the liver of uninfected or 9 wpi WT mice. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32973293/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen III alpha 1-COL3A1 Antibody-Immunohistochemistry-NB600-594-img0024.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Western blot for COL3A1 protein with PVDF protein staining to control for protein loading. Image collected and cropped by CiteAb from the following publication (mdpi.com/2076-3921/6/4/87), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Western-Blot-NB600-594-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen III alpha 1 expression in HT-1080 cells. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-594-img0008.jpg)
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human lung tissue.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NB600-594-img0013.jpg)
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human lung tissue](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NB600-594-img0014.jpg)
![Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen III (k, positive staining indicated by arrows) immunohistochemistry staining in 13 weeks old male offspring. Left panel = Sham, Middle panel = SE (cigarette smoke exposure), Right panel = SE+MQ (SE with MitoQ supplementation) bar = 50 um. Image collected and cropped by CiteAb from the following publication (mdpi.com/2072-6643/11/7/1669) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-NB600-594-img0020.jpg)
![Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen I and collagen III accumulate in C57BL/6 mice in response to radiation. Bladder strips from irradiated and control mice were stained for collagen I or for collagen III and imaged using fluorescent microscopy. (a) Representative images (100 x 100 um) of each mouse strain and treatment group are provided for collagen I and collagen III immunofluorescence. Collagen I antibody (NB600-450, RRID:AB_522923) and Collagen III antibody (NB600-594, RRID:AB_10001330). Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/abs/10.14814/phy2.14377) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-NB600-594-img0021.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free [NBP1-05119] - Western blot analysis on total proteins extracted from cells cultured with L-PRP or P-PRP. Collagen I protein band was robust in the P-PRP-treated group; collagen III alpha 1 protein band was more abundant in the L-PRP-treated cells. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/6/1/173) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-1E7-D7-Col3-BSA-Free-Western-Blot-NBP1-05119-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free [NBP1-05119] - Western blot analysis on total proteins extracted from cells cultured with L-PRP or P-PRP. Collagen I protein band was robust in the P-PRP-treated group; collagen III alpha 1 protein band was more abundant in the L-PRP-treated cells. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/6/1/173) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free [NBP1-05119] - Human bronchus tissue](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-1E7-D7-Col3-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05119-img0002.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Various extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Western-Blot-NBP2-15946-img0010.jpg "Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Various extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Human PAPILLARY CA_STROMAL CELLS, using Collagen III antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NBP2-15946-img0008.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Western-Blot-NBP3-15309-img0004.jpg "Western Blot: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Immunofluorescence analysis of NIH/3T3 cells using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Immunocytochemistry-Immunofluorescence-NBP3-15309-img0002.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Western-Blot-NBP3-15309-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Immunofluorescence analysis of RD cells using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Immunocytochemistry-Immunofluorescence-NBP3-15309-img0001.jpg)
![Immunoprecipitation: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug Collagen III alpha 1/COL3A1 antibody (NBP3-15309). Western blot was performed from the immunoprecipitate using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Immunoprecipitation-NBP3-15309-img0005.jpg)
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] - Collagen III antibody [HL1906] detects Collagen III protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. Collagen III stained by Collagen III antibody [HL1906] (NBP3-25419) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25419_rabbit-collagen-iii-alpha-1-col3a1-mab-hl1906-azide-bsa-free-2120249355527.jpg "Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] - Collagen III antibody [HL1906] detects Collagen III protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. Collagen III stained by Collagen III antibody [HL1906] (NBP3-25419) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [HL1906] (NBP3-25419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25419_rabbit-collagen-iii-alpha-1-col3a1-mab-hl1906-azide-bsa-free-212024940826.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [HL1906] (NBP3-25419) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25419_rabbit-collagen-iii-alpha-1-col3a1-mab-hl1906-azide-bsa-free-2120249371516.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] - Collagen III antibody [HL1906] detects Collagen III protein at cytoplasmic vesicle by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in ice-cold MeOH for 5 min. Green: Collagen III stained by Collagen III antibody [HL1906] (NBP3-25419) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25419_rabbit-collagen-iii-alpha-1-col3a1-mab-hl1906-azide-bsa-free-2120249432898.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Analysis in human cell lines HeLa and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Western-Blot-NBP1-84007-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Analysis in human cell lines HeLa and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84007-img0011.jpg)
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84007-img0008.jpg)
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Staining of human colorectal cancer shows weak to moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84007-img0009.jpg)
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Staining of human skin shows weak to moderate cytoplasmic positivity in fibroblasts.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84007-img0010.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267] - Western Blot of Collagen III alpha 1/COL3A1. Lane 1: Human Collagen III. Load: 100 ng per lane. Primary antibody: Collagen III Antibody at 1:1000 at 4C. Secondary antibody: DyLight(TM) 649 Goat anti-rabbit at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 138 kDa, 138 kDa.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Native-Protein-Western-Blot-NBP1-97267-img0004.jpg "Western Blot: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267] - Western Blot of Collagen III alpha 1/COL3A1. Lane 1: Human Collagen III. Load: 100 ng per lane. Primary antibody: Collagen III Antibody at 1:1000 at 4C. Secondary antibody: DyLight(TM) 649 Goat anti-rabbit at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 138 kDa, 138 kDa.")
![SDS-Page: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267] - SDS-PAGE Results of Collagen III alpha 1/COL3A1 Native protein. Lane 1: Collagen III alpha 1/COL3A1 Native protein Reduced [1.0ug]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Collagen III alpha 1/COL3A1 Native protein Non-Reduced [1.0ug]. 4-20% Gel Coomassie Stained. Observed: alpha1 bands.](http://images.novusbio.com/fullsize/Collagen III alpha 1-COL3A1 Native Protein-SDS-Page-NBP1-97267-img0005.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (SR1186) [NBP3-22424] - Western blot analysis using NBP3-22424 in (1) A431 cell lysate; (2) MCF7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22424_rabbit-collagen-iii-alpha-1-col3a1-mab-sr1186-57202315213813.jpg "Western Blot: Collagen III alpha 1/COL3A1 Antibody (SR1186) [NBP3-22424] - Western blot analysis using NBP3-22424 in (1) A431 cell lysate; (2) MCF7 cell lysate.")
![SDS-Page: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97244] - SDS-PAGE Results of Collagen III alpha 1/COL3A1 Native protein. Lane 1: Collagen III alpha 1/COL3A1 Native protein Reduced (7.0ug).Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Collagen III alpha 1/COL3A1 Native protein Non-Reduced (7.0ug). 7.5% Gel, Coomassie Stained. Observed MW: Type III trimer, dimer, monomer.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Native-Protein-SDS-Page-NBP1-97244-img0001.jpg "SDS-Page: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97244] - SDS-PAGE Results of Collagen III alpha 1/COL3A1 Native protein. Lane 1: Collagen III alpha 1/COL3A1 Native protein Reduced (7.0ug).Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Collagen III alpha 1/COL3A1 Native protein Non-Reduced (7.0ug). 7.5% Gel, Coomassie Stained. Observed MW: Type III trimer, dimer, monomer.")
![ELISA: Rat Collagen III alpha 1/COL3A1 ELISA Kit (Colorimetric) [NBP2-75860] - Samples were spiked with high concentrations of Rat Collagen III alpha 1/COL3A1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75860_rat-collagen-iii-alpha-1-col3a1-elisa-kit-colorimetric-132202416235769.jpg "ELISA: Rat Collagen III alpha 1/COL3A1 ELISA Kit (Colorimetric) [NBP2-75860] - Samples were spiked with high concentrations of Rat Collagen III alpha 1/COL3A1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Collagen III alpha 1/COL3A1 ELISA Kit (Colorimetric) [NBP2-75860] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75860_rat-collagen-iii-alpha-1-col3a1-elisa-kit-colorimetric-4320249173422.jpg)