| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44513 | |||
| Azide and BSA Free | NBP2-34569-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 9090-B2-01M | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF-141-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF-441-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1340-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47778-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11584 | |||
![Knockout Validated: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Knockout-Validated-NBP2-25208-img0005.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Macrophage polarization states. Distribution and proportion of CD86+ macrophages (brown) were identified using IHC procedures. Intensity for the color response to antibodies was visible in lung tissues from all groups. Population proportions for the stained cells as shown by red arrows were calculated as a fold change of the control. (d). The results were expressed as Mean +/- SD (n = 3). **: a p-value of < 0.01 vs NS, OVA/BUD and OVA/PCI except the number of CD86+ cells in OVA/PCI group. #: P < 0.05 vs NS and ##; P < 0.01 vs either NS or OVA/BUD group. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-020-1322-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunohistochemistry-NBP2-25208-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Immunofluorescence staining of PFA-fixed Ramos cells using followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunocytochemistry-Immunofluorescence-NBP2-25208-img0006.jpg)
![Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Effects of 20(S)-Rg3 on macrophage polarization in atherosclerotic lesions of diabetic ApoE-/- mice. Co-immunofluorescence staining for macrophage (anti-Moma-2 antibody, green), M1 marker CD86 (red) and DAPI (blue) (n = 5, respectively). Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/articles/10.3389/fphar.2018.00464/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunohistochemistry-NBP2-25208-img0010.jpg)
![Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of PFA-fixed Ramos cells. B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Flow-Cytometry-NBP2-25208-img0008.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunohistochemistry-Paraffin-NBP2-25208-img0004.jpg)
![Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of human PBMCs using B7-2/CD86 Antibody (BU63); Goat anti-Mouse IgG-CF488 (red); Isotype Control (green).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Flow-Cytometry-NBP2-25208-img0007.jpg)
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysateLane 2: Hela cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF-7 cell lysate](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-SJ20-00-Western-Blot-NBP2-67417-img0006.jpg "Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysateLane 2: Hela cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF-7 cell lysate")
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Staining CD86 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-SJ20-00-Immunocytochemistry-Immunofluorescence-NBP2-67417-img0004.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of paraffin-embedded human tonsil tissue using anti-CD86 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-SJ20-00-Immunohistochemistry-Paraffin-NBP2-67417-img0005.jpg)
![Flow Cytometry: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-SJ20-00-Flow-Cytometry-NBP2-67417-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Staining CD86 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-SJ20-00-Immunocytochemistry-Immunofluorescence-NBP2-67417-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Staining CD86 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-SJ20-00-Immunocytochemistry-Immunofluorescence-NBP2-67417-img0003.jpg)
![Flow Cytometry: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of K562 cells with CD86 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-SJ20-00-Flow-Cytometry-NBP2-67417-img0001.jpg)
![Knockout Validated: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-44514) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-C86-1146-Knockout-Validated-NBP2-44514-img0006.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-44514) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Analysis of Daudi Cell Lysate using CD86 Monoclonal Antibody (C86/1146).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-C86-1146-Western-Blot-NBP2-44514-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Confocal Immunofluorescence image of Raji cells using B7-2/CD86 Antibody (C86/1146) followed by goat anti-Mouse IgG conjugated with CF488 (green). Red Dot is used to label the Nuclei (red).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-C86-1146-Immunocytochemistry-Immunofluorescence-NBP2-44514-img0007.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Human Melanoma stained with CD86 Monoclonal Antibody (C86/1146).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-C86-1146-Immunohistochemistry-Paraffin-NBP2-44514-img0002.jpg)
![Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate from Jurkat, Ramos, KG-1, SUP-M2, HeLa, HDLM-2, HEK293T, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD86 recombinantmonoclonal antibody [BLR030F] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR030F-Western-Blot-NBP2-76417-img0007.jpg "Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate from Jurkat, Ramos, KG-1, SUP-M2, HeLa, HDLM-2, HEK293T, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD86 recombinantmonoclonal antibody [BLR030F] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Detection of human CD86 in FFPE HDLM-2 by ICC. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR030F-Immunocytochemistry-NBP2-76417-img0003.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Detection of human CD86 in FFPE seminoma by IHC. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR030F-Immunohistochemistry-Paraffin-NBP2-76417-img0002.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Detection of human CD86 in FFPE tonsil by IHC. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR030F-Immunohistochemistry-Paraffin-NBP2-76417-img0001.jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417) and rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR030F-Immunoprecipitation-NBP2-76417-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SPM600) [NBP2-44515] - Immunofluorescence staining of Ramos cells using B7-2/CD86 antibody (SPM600).; followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-SPM600-Immunocytochemistry-Immunofluorescence-NBP2-44515-img0002.jpg "Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SPM600) [NBP2-44515] - Immunofluorescence staining of Ramos cells using B7-2/CD86 antibody (SPM600).; followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.")
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (SPM600) [NBP2-44515] - Human Melanoma stained with CD86 Monoclonal Antibody (SPM600).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-SPM600-Immunohistochemistry-Paraffin-NBP2-44515-img0001.jpg)
![Western Blot: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of HDLM-2, MJ, SUP-M2, KG-1, MOLT-4, Ramos, Jurkat, HeLa, HEK293T, and RAW 264.7 lysate. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Western-Blot-NBP2-76416-img0002.jpg "Western Blot: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of HDLM-2, MJ, SUP-M2, KG-1, MOLT-4, Ramos, Jurkat, HeLa, HEK293T, and RAW 264.7 lysate. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.")
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 in FFPE HDLM-2 cells by ICC. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Immunocytochemistry-NBP2-76416-img0001.jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416) and rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Immunoprecipitation-NBP2-76416-img0003.jpg)