| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-75281 | |||
| DyLight 350 | NBP1-75635 | |||
| DyLight 488 | NBP1-75285 | |||
| DyLight 550 | NBP1-75636 | |||
| DyLight 594 | NBP1-75637 | |||
| DyLight 633 | NBP1-75638 | |||
| FITC | NBP1-75282 | |||
| HRP | NBP1-75283 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75281 | |||
| Pre-adsorbed | NBP1-75282 | |||
| Pre-adsorbed | NBP1-75283 | |||
| Pre-adsorbed | NBP1-75285 | |||
| Pre-adsorbed | NBP1-75635 | |||
| Pre-adsorbed | NBP1-75636 | |||
| Pre-adsorbed | NBP1-75637 | |||
| Pre-adsorbed | NBP1-75638 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-74819 | |||
| Biotin | NBP1-74820 | |||
| DyLight 488 | NBP1-74824 | |||
| DyLight 550 | NBP1-75606 | |||
| DyLight 594 | NBP1-75607 | |||
| DyLight 650 | NBP1-75609 | |||
| FITC | NBP1-74821 | |||
| HRP | NBP1-74822 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-74819 | |||
| Pre-adsorbed | NBP1-74820 | |||
| Pre-adsorbed | NBP1-74821 | |||
| Pre-adsorbed | NBP1-74822 | |||
| Pre-adsorbed | NBP1-74824 | |||
| Pre-adsorbed | NBP1-75606 | |||
| Pre-adsorbed | NBP1-75607 | |||
| Pre-adsorbed | NBP1-75609 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NB7184 | |||
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0012.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image using the DyLight 488 form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Image shows detection of PD-1 in mouse frozen tissue sections (salivary gland injected with human T cells) using DyLight™ 488 conjugated donkey anti-goat IgG (H+L) secondary antibody (green). Image from verified customer review. Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP2-60657-img0007.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): None. Image using the Texas Red form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0001.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Rabbit anti-Transferrin, Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST. Detection shown was using DyLight549 Donkey anti-Rabbit IgG, DyLight 488 Donkey anti-Mouse IgG, and DyLight 649 Donkey anti-Goat IgG at 1:10000 (in blocking buffer min RT). Blots were washed, rinsed in methanol and dried. Image using the DyLight 649 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Image shows anti-Histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was detected using a DyLight™ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP2-60657-img0006.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP2-60657-img0013.jpg)
![ELISA: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - ELISA Results of Donkey Donkey anti-Goat IgG (H+L) Secondary antibody (Pre-adsorbed) tested against purified Goat IgG MX7. Each well was coated in duplicate with 1.0 ug of Goat IgG as well as Chicken IgG, Guinea Porcine IgG, Hamster IgG, Equine IgG, Mouse IgG, Rabbit IgG, and Rat IgG. The working dilution is 1:56,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Donkey IgG Mx7 HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP2-60657-img0014.jpg)
![Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of PP4C in Huh4 cell lysate. Image from verified customer review. Image using the HRP format of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-75286-img0009.jpg "Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of PP4C in Huh4 cell lysate. Image from verified customer review. Image using the HRP format of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of human osteosarcoma cell using Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75286-img0006.jpg)
![Immunohistochemistry-Frozen: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - Analysis of frozen mouse tissue using DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-Frozen-NBP1-75286-img0008.jpg)
![Flow Cytometry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75286] - analysis of LM7 cells using Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) conjugated to DyLight 594. Image from verified customer review. Image using the DyLight 594 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Flow-Cytometry-NBP1-75286-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - West Nile virus infection of mosquito cells were stained with primary antibody followed by DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75279-img0006.jpg "Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - West Nile virus infection of mosquito cells were stained with primary antibody followed by DyLight 594 conjugated Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the DyLight 594 form of this antibody.")
![Immunohistochemistry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of mouse tissue. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-NBP1-75279-img0008.jpg)
![Flow Cytometry: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of human pancreatic cancer cells. Note: Need to be careful about bleeding into different filters since it might interfere with FITC. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Flow-Cytometry-NBP1-75279-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75279] - Analysis of human osteosarcoma cell line. Image from verified customer review. Image using the DyLight 550 form of Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-75279-img0005.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-74818] - Lysates of mouse embryo tissue loaded at the indicated concentrations. PVDF membrane was probed with 0.2 ug/mL goat anti-P-cadherin polyclonal antibody (AF761) followed by a 1:5000 Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) conjugated to HRP (NBP1-74822). Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-74818-img0004.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-74818] - Lysates of mouse embryo tissue loaded at the indicated concentrations. PVDF membrane was probed with 0.2 ug/mL goat anti-P-cadherin polyclonal antibody (AF761) followed by a 1:5000 Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) conjugated to HRP (NBP1-74822). Image using the HRP form of this antibody.")
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-74818] - Lysates of mouse embryo tissue loaded at the indicated concentrations. PVDF membrane was probed with 0.2 ug/mL goat anti-P-cadherin polyclonal antibody (AF761) followed by a 1:5000 donkey anti-goat IgG-HRP (NBP1-74822). Image using the HRP form of Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-74818-img0005.jpg)
![Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7056] - Rabbit anti-Transferrin, Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST. Detection shown was using DyLight549 Donkey anti-Rabbit IgG, DyLight 488 Donkey anti-Mouse IgG, and DyLight 649 Donkey anti-Goat IgG at 1:10000 (in blocking buffer min RT). Blots were washed, rinsed in methanol and dried. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NB120-7056-img0003.jpg "Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7056] - Rabbit anti-Transferrin, Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST. Detection shown was using DyLight549 Donkey anti-Rabbit IgG, DyLight 488 Donkey anti-Mouse IgG, and DyLight 649 Donkey anti-Goat IgG at 1:10000 (in blocking buffer min RT). Blots were washed, rinsed in methanol and dried. Image from the DyLight 488 version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7056] - Using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NB120-7056-img0007.jpg)
![Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7056] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NB120-7056-img0001.jpg)
![ELISA: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7056] - ELISA results of purified Donkey anti-Mouse IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed) tested against purified Mouse IgG. (Blue Line). Each well was coated in duplicate with 1.0 ug of antigen. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer. Using the Alkaline Phosphatase format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NB120-7056-img0006.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7056] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NB120-7056-img0008.jpg)
![Western Blot: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - DyLight(TM) dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 680 conjugate. Anti-TNFa was detected using a DyLight(TM) 800 conjugate. The image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72745-img0008.jpg "Western Blot: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - DyLight(TM) dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 680 conjugate. Anti-TNFa was detected using a DyLight(TM) 800 conjugate. The image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - Immunocytochemistry/Immunofluorescence: Donkey anti-Sheep IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-73002] Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72745-img0003.jpg)
![ELISA: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - ELISA results of purified Polyclonal Donkey anti-Sheep IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed) tested against Sheep IgG. Each well was coated in duplicate with 1.0ug of antigen. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Buffer. Using the Alkaline Phosphatase format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72745-img0004.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-72745-img0009.jpg)
![ELISA: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - ELISA Results of Donkey anti-Sheep IgG (H+L) Secondary antibody (Pre-adsorbed) tested against purified Sheep IgG Mx8. Each well was coated in duplicate with 1.0 ug of Sheep IgG [Red Line], Chicken IgG [Green Line], Guinea Porcine IgG [Blue Line], Hamster IgG [Purple Line], Equine IgG, Human IgG, Mouse IgG, Rabbit IgG, and Rat IgG. The working dilution of Sheep IgG MX8 is 1:49,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Donkey IgG HRP conjugated, and TMB substrate.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72745-img0010.jpg)
![Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72741] - Lane 1: Rabbit IgG Load: 50 ug per lane Secondary antibody: Rabbit IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72741-img0008.jpg "Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72741] - Lane 1: Rabbit IgG Load: 50 ug per lane Secondary antibody: Rabbit IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image from the DyLight 488 version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72741] - Immunocytochemistry/Immunofluorescence: Donkey anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72966] Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72741-img0006.jpg)
![Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72741] - Donkey anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 800] [NBP1-72970] - DyLight (R) dyes can be used for two-color Western Blot detection with low background and high signal](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72741-img0004.jpg)
![Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72741] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng of IgG, F(ab), F(c) and Serum, 25 ng of IgM. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) 1:7,500 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72741-img0009.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72741] - Donkey anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 800] [NBP1-72970]](http://images.novusbio.com/fullsize/Donkey-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-72741-img0005.jpg)