| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-304SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-305SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-904SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56705SS | |||
| Azide and BSA Free | NBP2-33243 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25028SS | |||
| Azide and BSA Free | NBP2-80549 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24541SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-03993SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24521SS | |||
![Depletion of PNUTS and NPAT induces a global DDR.(A) Induction of telomere dysfunction–induced foci (TIF) upon candidate knockdown in HeLa cells from Fig 5. Depletion of TRF2 was used as a positive control. 53BP1 was detected by indirect IF and telomeres by FISH with a Cy3-[CCCTAA]3 probe. (B) Frequency distribution of the number of TIFs per cell from (A). n indicates the number of cells analyzed for each condition and red lines mark the mean.](http://images.novusbio.com/fullsize/nb100-304_rabbit-polyclonal-53bp1-antibody-271220231261934.jpg)
![CO attenuates mitochondrial dysfunction-derived sterile inflammation through TFEB-mediated mitophagy and mitochondrial biogenesis.Mice were exposed to LPS/D-galactosamine (D-GalN) with or without CO (250 ppm, 2 h/day) pretreatment, the blood and liver samples were collected 2 h and 6 h (b–i) after LPS/D-GalN administration. a The levels of ALT and AST were determined. Data are shown as mean ± SEM (n = 5). ***P < 0.001. b Liver sections were stained with H&E for morphological evaluation. c The levels of serum IL-1 beta were measured by ELISA. Data are shown as mean ± SEM (n = 4). **P < 0.01. ***P < 0.001. d Cytosolic mtDNA amounts in liver tissues were measured by qRT-PCR. Data are shown as mean ± SEM (n = 4). *P < 0.05. e Cleaved caspase-1 in cell lysates isolated from liver tissues was analyzed by immunoblotting. f The level of nuclear TFEB in liver tissues was determined by immunoblotting. Lamin B served as the nuclear standard. g Mitochondrial fractionation in liver tissues was analyzed by immunoblotting using antibodies against PINK1, Parkin, and LC3B-II. COX IV was used as marker for mitochondrial fractions. h, i The levels of autophagy-related genes and lysosomal genes (h) or mitochondrial biogenesis-associated genes (i) expressed in liver tissues were analyzed by qRT-PCR. Data are shown as mean ± SEM (n = 4). *P < 0.05, **P < 0.01. ***P < 0.001. j A scheme for the mechanisms of attenuating LSP/D-GalN-induced liver failure by CO through maintaining the balance between mitophagy and mitochondrial biogenesis. CO/CORM inhibits complexes of the mitochondrial respiratory chain (1) and then induces mitochondrial ROS (mtROS) production (2). Increased mtROS leads to PERK activation in the mitochondria-associated membrane (MAM) (3). The phosphorylation of PERK by mtROS increases intracellular calcium concentration. The activation of calcineurin by increased [Ca2 + ]i causes dephosphorylation of TREB (4), followed by its translocation into the nucleus. The nuclear translocation of TFEB increases the expression of genes involved in mitophagy (5) and mitochondrial biogenesis (6) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30333475), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-304_rabbit-polyclonal-53bp1-antibody-2322024884423.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-305] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB100-305 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-305-img0010.jpg "Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-305] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB100-305 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![<b>Genetic Strategies Validation. </b>Knockdown Validated: 53BP1 Antibody [NB100-305] - Western blot showing an efficient siRNA-mediated knockdown of the indicated individual or combined proteins. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11023) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/53BP1-Antibody-Knockdown-Validated-NB100-305-img0013.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: 53BP1 Antibody - BSA Free [NB100-305] - 53BP1 Antibody [NB100-305] - Exosomes modulate the repair of DNA DSBs in irradiated recipient cells. Representative images of 53BP1 foci in BHY cells 6 hours after 2 Gy and transfer of BHY exosomes isolated 24 hours after irradiation with 0, 3, 6 or 9 Gy (53BP1 foci green, nuclei blue). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0152213), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-305-img0008.jpg)
![Immunohistochemistry: 53BP1 Antibody [NB100-305] - Immunohistochemical staining of placental villi with 53BP1 Antibody (Catalog #NB100-305) at 40X magnification.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunohistochemistry-NB100-305-img0005.jpg)
![Western Blot: 53BP1 Antibody [NB100-305] - Western blot of whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8 percent trisacetate gel. A band appears at approximately the theoretical molecular weight of 214 kDa.](http://images.novusbio.com/fullsize/53BP1-Antibody-Western-Blot-NB100-305-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-305] - HCC1937 cells (human) were treated with PARPi for 96 h. Nuclei are stained in blue (DAPI) and 53BP1 antibody (foci in green). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-305-img0012.jpg)
![Flow Cytometry: 53BP1 Antibody [NB100-305] - An intracellular stain was performed on Ntera2 cells with 53BP1 Antibody NB100-305 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/53BP1-Antibody-Flow-Cytometry-NB100-305-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-305] - Paraformaldehyde (4%) fixed Saos-2 cells (human osteosarcoma cell line) were stained with anti-53BP1 antibody followed by Alexa Fluor 488 secondary antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-305-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-305] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB100-305 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-305-img0009.jpg)
![Flow Cytometry: 53BP1 Antibody [NB100-305] - An intracellular stain was performed on HeLa cells with 53BP1 Antibody (Catalog #NB100-305AF488) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/53BP1-Antibody-Flow-Cytometry-NB100-305-img0006.jpg)
![RAP1 protects telomeres against DNA damageWestern blotting showing the expression of RAP1 and TRF2 in MRC-5 cells of different population doublings (PD). Senescent cells (PD 72) were left in culture for at least 4 weeks before harvesting for analysis.ChIP analysis of young (PD 30) and senescent (PD 72) MRC-5 cells performed with either anti-RAP1, anti-TRF2, or an IgG antibody. The immunoprecipitated and input products were loaded into a slot blot membrane and hybridized with a telomere probe, and stripped and hybridized to an Alu probe in order to determine unspecific binding. Quantification was performed by normalizing the telomere signal of the immunoprecipitated to that of the input. Data represent mean +/- SD of three biological replicates.Immunofluorescence detection of 53BP1 (red) and a FISH probe staining telomeres (green) in young (PD 26), pre-senescent (PD 66), and senescent (PD 72 + 4 weeks) MRC-5 fibroblasts. The upper graph shows the percentage of telomere co-localizing with 53BP1 (TIFs). Total DNA damage was measured by immunofluorescence detection of 53BP1. The lower graph shows the total number of 53BP1 foci per nucleus. Approximately 40-50 cells were analyzed per replicate and per condition. Bars represent SEM of two biological replicates.Data information: Statistical analyses were performed using Mann-Whitney U-test (*P 0.05, n = 18; group (mean % of cells with more than five 53BP1 ionizing radiation-induced foci +/−SE); unpaired t test]. See text for details. (C) 53BP1 accumulates at infrequent endogenous chromosomal breaks (indicated by asterisks; no ionizing radiation treatment) in presence or absence of TPX2. (D) Cell cycle profiles of control and TPX2 miRNA expressing HeLa cell cultures obtained via flow cytometry (n = 2). Note that the slight ∼5% increase in the G2/M fraction upon TPX2 depletion can not account for the defect in 53BP1 ionizing radiation-induced foci formation exhibited by ∼55% of TPX2 miRNA expressing cells. See text for details. Error bars represent SE in (B) and SDEV in (D). n = # of independent experiments; NS: non-significant, * p<0.05, ** p<0.01, *** p<0.001; IR: ionizing radiation. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-305_rabbit-polyclonal-53bp1-antibody-23220248272420.jpg)
![Western Blot: 53BP1 Antibody - BSA Free [NB100-904] - WRAP53beta plays an important role in recruitment of factors involved in HR and NHEJ to DNA breaks in A2780 cells. A2780 cells were transfected with siControl or siWRAP53#2 oligonucleotides for 48 h; exposed to IR (6 Gy), and 1 h later immunostained for WRAP53beta, gamma-H2AX, MDC1, RNF168, 53BP1, RAD51 and beta-actin. We could not assess the protein levels of BRCA1 due to a lack of antibodies that work for western blotting. The observed molecular weight is 275 kDa and the theoretical molecular weight for the whole endogenous protein is 214 kDa. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2015250) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/53BP1-Antibody---BSA-Free-Western-Blot-NB100-904-img0015.jpg "Western Blot: 53BP1 Antibody - BSA Free [NB100-904] - WRAP53beta plays an important role in recruitment of factors involved in HR and NHEJ to DNA breaks in A2780 cells. A2780 cells were transfected with siControl or siWRAP53#2 oligonucleotides for 48 h; exposed to IR (6 Gy), and 1 h later immunostained for WRAP53beta, gamma-H2AX, MDC1, RNF168, 53BP1, RAD51 and beta-actin. We could not assess the protein levels of BRCA1 due to a lack of antibodies that work for western blotting. The observed molecular weight is 275 kDa and the theoretical molecular weight for the whole endogenous protein is 214 kDa. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2015250) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody - BSA Free [NB100-904] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB100-904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/53BP1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-904-img0021.jpg)
![Immunohistochemistry: 53BP1 Antibody - BSA Free [NB100-904] - Staining of 53BP1 in human renal cancer cells using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/53BP1-Antibody---BSA-Free-Immunohistochemistry-NB100-904-img0008.jpg)
![Flow Cytometry: 53BP1 Antibody - BSA Free [NB100-904] - An intracellular stain was performed on Ntera2 cells with 53BP1 Antibody NB100-904 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/53BP1-Antibody---BSA-Free-Flow-Cytometry-NB100-904-img0022.jpg)
![Western Blot: 53BP1 Antibody - BSA Free [NB100-904] - Western blot analysis of untreated U2OS cell lysate blotted with 53BP1 Antibody. The observed molecular weight here is ~220 kDa, and the theoretical molecular weight is 214 kDa.](http://images.novusbio.com/fullsize/53BP1-Antibody---BSA-Free-Western-Blot-NB100-904-img0011.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: 53BP1 Antibody - BSA Free [NB100-904] - AR suppression treatment suppresses DNA damage repair. (A) Cells were treated with AR suppression for 24 h followed by 2 Gy of IR treatment. Representative images of phospho-H2A histone family member X ( -H2AX) and p53-binding protein 1 (53BP1) staining 24 h post-IR of LNCaP cells (scale bar 10 um). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31635359/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/53BP1 Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NB100-904-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody - BSA Free [NB100-904] - Immunofluorescence of 53BP1 Antibody in HeLa cells. Image from verified customer review.](http://images.novusbio.com/fullsize/53BP1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-904-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody - BSA Free [NB100-904] - Immunofluorescence of 53BP1 Antibody in C33A cells. Image from verified customer review.](http://images.novusbio.com/fullsize/53BP1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-904-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody - BSA Free [NB100-904] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with 53BP1 Antibody conjugated to Alexa Fluor 488 (Catalog #NB100-904AF488) at 10ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/53BP1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-904-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody - BSA Free [NB100-904] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-53BP1 Antibody NB100-904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/53BP1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-904-img0019.jpg)
![Flow Cytometry: 53BP1 Antibody - BSA Free [NB100-904] - An intracellular stain was performed on HeLa cells (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/53BP1-Antibody---BSA-Free-Flow-Cytometry-NB100-904-img0014.jpg)
![Flow Cytometry: 53BP1 Antibody - BSA Free [NB100-904] - An intracellular stain was performed on HeLa cells with 53BP1 Antibody (Catalog #NB100-904AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/53BP1-Antibody---BSA-Free-Flow-Cytometry-NB100-904-img0017.jpg)
![Flow Cytometry: 53BP1 Antibody - BSA Free [NB100-904] - An intracellular stain was performed on HeLa cells with 53BP1 Antibody (Catalog #NB100-904C) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/53BP1-Antibody---BSA-Free-Flow-Cytometry-NB100-904-img0018.jpg)
![Knockout Validated: TBK1 Antibody (108A429) [NB100-56705] - Western blot using lysates from U20S parental cell line and TBK1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse/Rat TBK1 Monoclonal Antibody (Catalog # NB100-56705) at 1 ug/ul O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band at 83 kDa was detected for TBK1 in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Knockout-Validated-NB100-56705-img0023.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: TBK1 Antibody (108A429) [NB100-56705] - Western blot using lysates from U20S parental cell line and TBK1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse/Rat TBK1 Monoclonal Antibody (Catalog # NB100-56705) at 1 ug/ul O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band at 83 kDa was detected for TBK1 in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Simple Western: TBK1 Antibody (108A429) [NB100-56705] - Lane view shows a specific band for NAK/TBK1 in 0.5 mg/ml of Daudi (left) and Raw 264.7 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Simple-Western-NB100-56705-img0014.jpg)
![Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Analysis of FFPE human testis tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:200. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Immunohistochemistry-Paraffin-NB100-56705-img0021.jpg)
![Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis using Azide and BSA Free version of NB100-56705. Lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2) probed with TBK1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Western-Blot-NB100-56705-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). A dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Immunocytochemistry-Immunofluorescence-NB100-56705-img0013.jpg)
![Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Immunohistochemistry: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Staining on human testis (Formalin-Fixed Paraffin-Embedded (FFPE)) Image from the Azide and BSA Free version of this antibody.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Immunohistochemistry-Paraffin-NB100-56705-img0022.jpg)
![Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis of Human Daudi (A) and mouse RAW (B) cell lysate probed with TBK1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Western-Blot-NB100-56705-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TBK1 at 20 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Immunocytochemistry-Immunofluorescence-NB100-56705-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (6B3E10) [NBP2-25028] - Staining of human U-2 OS cells using anti-53BP1 antibody. Bottom images: human U-2 OS cells were treated with EPE to induce DNA-damage and showed increase in nuclear spots after the induction of DNA damage with EPE. Image from verified customer review.](http://images.novusbio.com/fullsize/53BP1-Antibody-6B3E10-Immunocytochemistry-Immunofluorescence-NBP2-25028-img0006.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (6B3E10) [NBP2-25028] - Staining of human U-2 OS cells using anti-53BP1 antibody. Bottom images: human U-2 OS cells were treated with EPE to induce DNA-damage and showed increase in nuclear spots after the induction of DNA damage with EPE. Image from verified customer review.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: 53BP1 Antibody (6B3E10) [NBP2-25028] - Analysis of paraffin-embedded colon cancer tissues using 53BP1 Antibody with DAB staining.](http://images.novusbio.com/fullsize/53BP1-Antibody-6B3E10-Immunohistochemistry-NBP2-25028-img0004.jpg)
![Western Blot: 53BP1 Antibody (6B3E10) [NBP2-25028] - Western blot analysis using against HEK293 (1) and 53BP1 (AA: 574-773)-hIgGFc transfected HEK293 (2) cell lysate. The observed molecular weight is ~52 kDa and the theoretical molecular weight of the whole endogenous protein is 214 kDa.](http://images.novusbio.com/fullsize/53BP1-Antibody-6B3E10-Western-Blot-NBP2-25028-img0002.jpg)
![Flow Cytometry: 53BP1 Antibody (6B3E10) [NBP2-25028] - Analysis of HepG2 cells using 53BP1 Antibody (green) and negative control (purple).](http://images.novusbio.com/fullsize/53BP1-Antibody-6B3E10-Flow-Cytometry-NBP2-25028-img0003.jpg)
![Immunohistochemistry: 53BP1 Antibody (6B3E10) [NBP2-25028] - Analysis of paraffin-embedded endometrial cancer tissues using 53BP1 Antibody with DAB staining.](http://images.novusbio.com/fullsize/53BP1-Antibody-6B3E10-Immunohistochemistry-NBP2-25028-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (6B3E10) [NBP2-25028] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-53BP1 Antibody [6B3E10] NBP2-25028 at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Beta tubulin NB600-936 was used as a co-stain at a 1:1000 dilution and detected with an anti-rabbit DyLight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/53BP1-Antibody-6B3E10-Immunocytochemistry-Immunofluorescence-NBP2-25028-img0007.jpg)
![Flow Cytometry: 53BP1 Antibody (6B3E10) [NBP2-25028] - An intracellular stain was performed on HeLa cells with 53BP1 (6B3E10) Antibody NBP2-25028 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/53BP1-Antibody-6B3E10-Flow-Cytometry-NBP2-25028-img0011.jpg)
![Western Blot: 53BP1 Antibody (6B3E10) [NBP2-25028] - Western blot analysis using 53BP1 Antibody against human 53BP1 recombinant protein. The expected molecular weight is 47.6 kDa which is demonstrated in this analysis, and the theoretical molecular weight of the whole endogenous protein is 214 kDa.](http://images.novusbio.com/fullsize/53BP1-Antibody-6B3E10-Western-Blot-NBP2-25028-img0001.jpg)
![Flow Cytometry: 53BP1 Antibody (6B3E10) [NBP2-25028] - An intracellular stain was performed on Ntera2 cells with 53BP1 Antibody [6B3E10] NBP2-25028 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/53BP1-Antibody-6B3E10-Flow-Cytometry-NBP2-25028-img0010.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Brain Cerebellum Purkinje Neuron](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0010.jpg "Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Brain Cerebellum Purkinje Neuron")
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human tonsil.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0004.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human colon, carcinoma.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0006.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Human Lymph Node, Non-Hodgkins Lymphoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0007.jpg)
![Immunohistochemistry-Frozen: CXCR4 Antibody [NLS1380] - Rat brain tissue sections. Rat Nucleus Accumbens treated with MDPV. Images taken with fluorescent microscope. Primary antibody was diluted to 1:200. Secondary antibody used was donkey anti-Rabbit Alexa Fluor 594 at 1:500. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Frozen-NLS1380-img0008.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Lung, small cell carcinoma](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0009.jpg)
![Western Blot: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody analysis of p65 NLS in Jurkat lysate using p65 NLS antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-NLS-Antibody-Western-Blot-NBP2-24541-img0008.jpg "Western Blot: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody analysis of p65 NLS in Jurkat lysate using p65 NLS antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 NLS Antibody [NBP2-24541] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-RelA/NFkB p65 NLS Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-NLS-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24541-img0011.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody Formalin-fixed, paraffin-embedded human liver stained with p65 NLS antibody at 10 ug/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-NLS-Antibody-Immunohistochemistry-Paraffin-NBP2-24541-img0010.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody analysis of p65 NLS in formalin-fixed, paraffin-embedded human HeLa cells (top left) and LPS-stimulated HeLa cells (bottom left, right) using p65 NLS antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-NLS-Antibody-Immunohistochemistry-Paraffin-NBP2-24541-img0009.jpg)
![Immunohistochemistry-Paraffin: TAS1R3 Antibody [NLS5060] - Tongue, taste receptor](http://images.novusbio.com/fullsize/TAS1R3-Antibody-Immunohistochemistry-Paraffin-NLS5060-img0006.jpg "Immunohistochemistry-Paraffin: TAS1R3 Antibody [NLS5060] - Tongue, taste receptor")
![Immunohistochemistry-Paraffin: TAS1R3 Antibody [NLS5060] - Analysis of anti-T1R3 / TAS1R3 antibody with tongue, squamous epithelium at 17 ug/ ml.](http://images.novusbio.com/fullsize/TAS1R3-Antibody-Immunohistochemistry-Paraffin-NLS5060-img0001.jpg)
![Immunohistochemistry-Paraffin: TAS1R3 Antibody [NLS5060] - Analysis of anti-T1R3 / TAS1R3 antibody with human lung, non-small cell carcinoma.](http://images.novusbio.com/fullsize/TAS1R3-Antibody-Immunohistochemistry-Paraffin-NLS5060-img0003.jpg)
![Immunohistochemistry-Paraffin: TAS1R3 Antibody [NLS5060] - Human Skin, Melanoma tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/TAS1R3-Antibody-Immunohistochemistry-Paraffin-NLS5060-img0004.jpg)
![Immunohistochemistry-Paraffin: TAS1R3 Antibody [NLS5060] - Tongue, taste receptor](http://images.novusbio.com/fullsize/TAS1R3-Antibody-Immunohistochemistry-Paraffin-NLS5060-img0005.jpg)
![Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HDAC9-Antibody-Western-Blot-NBP2-03993-img0004.jpg "Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: HIPK3 Antibody [NBP2-24521] - Analysis of HIPK3 in mouse testis lysate in the 1) absence, 2) presence of immunizing peptide and 3) rat testis using this antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HIPK3-Antibody-Western-Blot-NBP2-24521-img0003.jpg "Western Blot: HIPK3 Antibody [NBP2-24521] - Analysis of HIPK3 in mouse testis lysate in the 1) absence, 2) presence of immunizing peptide and 3) rat testis using this antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: HIPK3 Antibody [NBP2-24521] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-HIPK3 [NBP2-24521] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HIPK3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24521-img0006.jpg)
![Immunohistochemistry-Paraffin: HIPK3 Antibody [NBP2-24521] - Analysis of human testis using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/HIPK3-Antibody-Immunohistochemistry-Paraffin-NBP2-24521-img0005.jpg)
![Immunohistochemistry-Paraffin: HIPK3 Antibody [NBP2-24521] - Analysis of HIPK3 in formalin-fixed, paraffin-embedded mouse testis tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/HIPK3-Antibody-Immunohistochemistry-Paraffin-NBP2-24521-img0001.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Colon, Carcinoma](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0010.jpg "Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Colon, Carcinoma")
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Analysis of anti-PTGER4 / EP4 antibody with human colon, ganglion at 2 ug/ml.](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0003.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Human Lung, Adenocarcinoma tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0004.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Human Prostate, Carcinoma tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0005.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Human prostate, benign prostatic hyperplasia tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0007.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Human Pancreas, Carcinoma tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0006.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Breast, Carcinoma](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0008.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Brain, Glioblastoma](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0009.jpg)
![Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Eye, Retina, Ganglion cells.](http://images.novusbio.com/fullsize/mGluR6-Antibody-Immunohistochemistry-Paraffin-NLS4655-img0010.jpg "Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Eye, Retina, Ganglion cells.")
![Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Analysis of anti-GRM6 / MGLUR6 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/mGluR6-Antibody-Immunohistochemistry-Paraffin-NLS4655-img0005.jpg)
![Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/mGluR6-Antibody-Immunohistochemistry-Paraffin-NLS4655-img0006.jpg)
![Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/mGluR6-Antibody-Immunohistochemistry-Paraffin-NLS4655-img0007.jpg)
![Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/mGluR6-Antibody-Immunohistochemistry-Paraffin-NLS4655-img0008.jpg)
![Immunohistochemistry-Paraffin: mGluR6 Antibody [NLS4655] - Eye, Retina](http://images.novusbio.com/fullsize/mGluR6-Antibody-Immunohistochemistry-Paraffin-NLS4655-img0009.jpg)