| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6896-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41237-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB68961-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6896-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41274-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79942 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79940 | |||
![Western Blot: Vinculin Antibody (hVIN-1) [NB600-1293] - Cell line lysates were separated on SDS-PAGE and probed with 1:200 Monoclonal Anti-Vinculin Clone: hVIN-1. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.Lanes:1.HeLa2.COS73.NIH-3T34.RAT25.CHO6.MDBK7.MDCK](http://images.novusbio.com/fullsize/Vinculin-Antibody-hVIN-1-Western-Blot-NB600-1293-img0003.jpg "Western Blot: Vinculin Antibody (hVIN-1) [NB600-1293] - Cell line lysates were separated on SDS-PAGE and probed with 1:200 Monoclonal Anti-Vinculin Clone: hVIN-1. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.Lanes:1.HeLa2.COS73.NIH-3T34.RAT25.CHO6.MDBK7.MDCK")
![Immunocytochemistry/Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Immunofluorescence images of LM8 cells treated with 0 nM, 1 nM, or 10 nM eribulin and stained for vinculin (red) and nucleus (blue) (left). Dotted line shows the cell shape. Scale bar: 10 um. Quantitative analysis of the area of vinculin staining (right). Values are mean +/- SEM (less than or equal to 30 cells per group). **P =30 cells per group). **P < 0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30719211), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize/nb600-1293_mouse-monoclonal-vinculin-antibody-hvin-1-29122023121464.jpg)
![Western Blot: Vinculin Antibody [NBP2-20859] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-20859-img0015.jpg "Western Blot: Vinculin Antibody [NBP2-20859] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Vinculin Antibody [NBP2-20859] - Vinculin antibody detects Vinculin protein at focal adhesion by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Vinculin stained by Vinculin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20859-img0013.jpg)
![Immunohistochemistry-Paraffin: Vinculin Antibody [NBP2-20859] - Human hepatoma, using Vinculin antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunohistochemistry-Paraffin-NBP2-20859-img0010.jpg)
![Western Blot: Vinculin Antibody [NBP2-20859] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-20859-img0005.jpg)
![Western Blot: Vinculin Antibody [NBP2-20859] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-20859-img0011.jpg)
![Western Blot: Vinculin Antibody [NBP2-20859] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-20859-img0012.jpg)
![Immunohistochemistry-Paraffin: Vinculin Antibody [NBP2-20859] - Mouse testis. Vinculin antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunohistochemistry-Paraffin-NBP2-20859-img0009.jpg)
![Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Analysis in human smooth muscle and cerebral cortex tissues using NBP1-87495 antibody. Corresponding VCL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunohistochemistry-Paraffin-NBP1-87495-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Analysis in human smooth muscle and cerebral cortex tissues using NBP1-87495 antibody. Corresponding VCL RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Vinculin Antibody [NBP1-87495] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VCL antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP1-87495-img0012.jpg)
![Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Stainig of human cerebral cortex shows no positivity in neuronal cells.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunohistochemistry-Paraffin-NBP1-87495-img0019.jpg)
![Western Blot: Vinculin Antibody [NBP1-87495] - Analysis in human heart tissue.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP1-87495-img0006.jpg)
![Western Blot: Vinculin Antibody [NBP1-87495] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP1-87495-img0007.jpg)
![Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunohistochemistry-Paraffin-NBP1-87495-img0015.jpg)
![Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Stainig of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunohistochemistry-Paraffin-NBP1-87495-img0016.jpg)
![Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunohistochemistry-Paraffin-NBP1-87495-img0017.jpg)
![Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Staining of human testis shows strong cytoplasmic postivity in cells in seminiferious ducts.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunohistochemistry-Paraffin-NBP1-87495-img0018.jpg)
![Western Blot: Vinculin Antibody [NBP2-41237] - Western Blot Validation in Mouse 3T3/balb Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41237 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-41237-img0007.jpg "Western Blot: Vinculin Antibody [NBP2-41237] - Western Blot Validation in Mouse 3T3/balb Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41237 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry: Vinculin Antibody - BSA Free [NBP2-41237] - Vinculin Antibody [NBP2-41237] - Rat small intestine tissue with Vinculin antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunofluorescence-NBP2-41237-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Vinculin Antibody [NBP2-41237] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41237 (2 ug/mL), Vinculin from Company B (1 ug/mL), Vinculin NBP2-41274 (1 ug/mL), and beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-41237-img0004.jpg)
![Western Blot: Vinculin Antibody [NBP2-41237] - Western Blot Validation in Human PC-3 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41237 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-41237-img0005.jpg)
![Western Blot: Vinculin Antibody [NBP2-41237] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: Vinculin NBP2-41237 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-41237-img0006.jpg)
![Immunohistochemistry: Vinculin Antibody [NBP2-41237] - Rat small intestine tissue with Vinculin antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunohistochemistry-NBP2-41237-img0002.jpg)
![Western Blot: Vinculin Antibody [NBP2-41274] - Western Blot Validation in PC3 Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: Vinculin NBP2-41274 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-41274-img0007.jpg "Western Blot: Vinculin Antibody [NBP2-41274] - Western Blot Validation in PC3 Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: Vinculin NBP2-41274 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/Immunofluorescence: Vinculin Antibody [NBP2-41274] - Staining of Vinculin in Jurkat cells with Vinculin antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41274-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Vinculin Antibody [NBP2-41274] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41237 (2 ug/mL), Vinculin from Company B (1 ug/mL), Vinculin NBP2-41274 (1 ug/mL), and beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-41274-img0005.jpg)
![Western Blot: Vinculin Antibody [NBP2-41274] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41274 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-41274-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Vinculin Antibody [NBP2-41274] - Staining of Vinculin in Jurkat cells with Vinculin antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41274-img0003.jpg)
![Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808] - Western Blot Analysis of human U-87, THP-1 cell lysate using Vinculin Antibody (VCL/2575).](http://images.novusbio.com/fullsize/Vinculin-Antibody-VCL-2575-Western-Blot-NBP2-79808-img0004.jpg "Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808] - Western Blot Analysis of human U-87, THP-1 cell lysate using Vinculin Antibody (VCL/2575).")
![Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2575) [NBP2-79808] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Vinculin Antibody (VCL/2575).](http://images.novusbio.com/fullsize/Vinculin-Antibody-VCL-2575-Immunohistochemistry-Paraffin-NBP2-79808-img0001.jpg)
![Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808] - Western Blot Analysis of human A431, K562, NIH3T3 cell lysate using Vinculin Antibody (VCL/2575).](http://images.novusbio.com/fullsize/Vinculin-Antibody-VCL-2575-Western-Blot-NBP2-79808-img0003.jpg)
![Protein Array: Vinculin Antibody (VCL/2575) [NBP2-79808] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vinculin Antibody (VCL/2575) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Vinculin-Antibody-VCL-2575-Protein-Array-NBP2-79808-img0002.jpg)
![Western Blot: Vinculin Antibody (VIN-11-5) [NB120-11193] - Chicken gizzard cell extract was separated on SDS-PAGE and probed with NB120-11193. The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBT/BCIP substrate. Antibody dilution 1/200.](http://images.novusbio.com/fullsize/Vinculin-Antibody-VIN-11-5-Western-Blot-NB120-11193-img0006.jpg "Western Blot: Vinculin Antibody (VIN-11-5) [NB120-11193] - Chicken gizzard cell extract was separated on SDS-PAGE and probed with NB120-11193. The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBT/BCIP substrate. Antibody dilution 1/200.")
![Immunocytochemistry/Immunofluorescence: Vinculin Antibody (VIN-11-5) [NB120-11193] - Double labeling of an isolated smooth muscle cell (chicken gizzard) with Monoclonal Anti-Vinculin, clone no. VIN-11-5 (green) and Anti-alpha Actinin (red).](http://images.novusbio.com/fullsize/Vinculin-Antibody-VIN-11-5-Immunocytochemistry-Immunofluorescence-NB120-11193-img0005.jpg)
![Western Blot: Vinculin Antibody (VCL/2572) [NBP2-79806] - Western Blot Analysis of U-87 and THP-1 cell lysate using Vinculin Antibody (VCL/2572).](http://images.novusbio.com/fullsize/Vinculin-Antibody-VCL-2572-Western-Blot-NBP2-79806-img0003.jpg "Western Blot: Vinculin Antibody (VCL/2572) [NBP2-79806] - Western Blot Analysis of U-87 and THP-1 cell lysate using Vinculin Antibody (VCL/2572).")
![Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2572) [NBP2-79806] - Formalin-fixed, paraffin-embedded human Testis stained with Vinculin Antibody (VCL/2572).](http://images.novusbio.com/fullsize/Vinculin-Antibody-VCL-2572-Immunohistochemistry-Paraffin-NBP2-79806-img0001.jpg)
![Protein Array: Vinculin Antibody (VCL/2572) [NBP2-79806] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vinculin Antibody (VCL/2572). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Vinculin-Antibody-VCL-2572-Protein-Array-NBP2-79806-img0002.jpg)
![SDS-Page: Recombinant Human Vinculin Protein [H00007414-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00007414-P01-1.jpg "SDS-Page: Recombinant Human Vinculin Protein [H00007414-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")