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26 Results for "novus"
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NB100-56618

TRAILR2/TNFRSF10B Antibody

(1 Review)
(42 Publications)
0.1 mg
NB100-56618
Western Blot: TRAILR2/TNFRSF10B Antibody [NB100-56618] - TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Total protein from HL-60, HepG2, HCT-116 and human placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TRAIL R2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Hu, Mu
Species Glossary
Reactivity: Hu, Mu
WB, Flow, ICC/IF, IHC
Applications: WB, Flow, ICC/IF, IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
IMG-120A
Alt Catalog# IMG-120A
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
         
Validated by:          
Available Formulations
Formulation Catalog # Availability Price  
Azide Free NB100-55744
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AF631

TRAILR2/TNFRSF10B Antibody [Unconjugated]

(32 Publications)
100 μg
AF631
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human TRAIL R2/TNFRSF10B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF631) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TRAIL R2/TNFRSF10B at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Hu
Species Glossary
Reactivity: Hu
WB, Func
Applications: WB, Func
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF631
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF631-SP
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MAB6311

TRAILR2/TNFRSF10B Antibody (71908) [Unconjugated]

(24 Publications)
500 μg
MAB6311
Flow cytometry studies of scFv-Fc-scTRAIL molecules and Fc-scTRAIL on Colo205 and HCT116 cells(A) Expression levels of EGFR, HER2, HER3, EpCAM, and TRAIL-receptors were investigated after treatment of the cells with medium or 250 ng/ml BZB for 16 h. (B) Serial dilutions of the molecules were analyzed. (C) 20 nM scTRAIL units were analyzed after preincubation of the cells with PBA or a 200-fold molar excess of the respective blocking antibody. Binding of Fc-scTRAIL was measured in the presence of all blocking antibodies separately and is here represented as the mean of blocking with all different antibodies. Bound molecules were detected via anti-FLAG-PE. relative MFI, relative median fluorescence intensity. Pairwise comparisons were performed by unpaired t test (two-tailed; *P 0.05). Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.24379), licensed under a CC-BY license. Not internally tested by R&D Systems.
Hu
Species Glossary
Reactivity: Hu
Flow, CyTOF-ready
Applications: Flow, CyTOF-ready
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB6311U-100UG
Alexa Fluor 405 FAB6311V-100UG
Alexa Fluor 488 FAB6311G-025
Alexa Fluor 488 FAB6311G-100
Alexa Fluor 532 FAB6311X
Alexa Fluor 594 FAB6311T-100UG
Alexa Fluor 700 FAB6311N-025
Alexa Fluor 700 FAB6311N-100
Alexa Fluor 750 FAB6311S-100UG
Allophycocyanin FAB6311A-025
Allophycocyanin FAB6311A-100
Allophycocyanin/Cy7 FAB6311APCCY7
Biotin FAB6311B
DyLight 350 FAB6311D
DyLight 405 FAB6311E
DyLight 488 FAB6311K
DyLight 550 FAB6311L
DyLight 594 FAB6311M
DyLight 650 FAB6311W
DyLight 680 FAB6311Y
DyLight 755 FAB6311Z
FITC FAB6311F
HRP FAB6311H
Janelia Fluor 549 FAB6311I
Janelia Fluor 646 FAB6311J
PE/Atto594 FAB6311PEATT594
PE/Cy5.5 FAB6311PECY55
PE/Cy7 FAB6311PECY7
Phycoerythrin FAB6311P-100
Phycoerythrin FAB6311P-025
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB6311-SP
Carrier Free FAB6311N-100
Carrier Free FAB6311N-025
Carrier Free FAB6311A-100
Carrier Free FAB6311A-025
Carrier Free FAB6311G-100
Carrier Free FAB6311G-025
Carrier Free FAB6311P-100
Carrier Free FAB6311P-025
Carrier Free FAB6311V-100UG
Carrier Free FAB6311U-100UG
Carrier Free FAB6311T-100UG
Carrier Free FAB6311S-100UG
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MAB631

TRAILR2/TNFRSF10B Antibody (71903) [Unconjugated]

(17 Publications)
100 μg
MAB631
Human TRAIL R2/TNFRSF10B Monoclonal Antibody induces apoptosis of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner, as measured by Resazurin (Catalog # AR002) . The ED50 for this effect is typically 2‑12 ng/mL.
Hu
Species Glossary
Reactivity: Hu
Func
Applications: Func
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB631-SP
Carrier Free MAB631-500
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AF721

TRAILR2/TNFRSF10B Antibody [Unconjugated]

(5 Publications)
100 μg
AF721
In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRAIL R2/TNFRSF10B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF721). The ND50 is typically 0.4-1.2 µg/mL.
Mu
Species Glossary
Reactivity: Mu
WB, Flow, B/N, CyTOF-ready
Applications: WB, Flow, B/N, CyTOF-ready
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF721
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF721-SP
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NBP2-46148

TRAILR2/TNFRSF10B Antibody (OTI2A5)

0.1 ml
NBP2-46148
Western Blot: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF10B.
Hu, Mu, Pm
Species Glossary
Reactivity: Hu, Mu, Pm
WB, IHC
Applications: WB, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 NBP2-74578AF350
Alexa Fluor 405 NBP2-74578AF405
Alexa Fluor 488 NBP2-74578AF488
Alexa Fluor 532 NBP2-74578AF532
Alexa Fluor 594 NBP2-74578AF594
Alexa Fluor 647 NBP2-74578AF647
Alexa Fluor 700 NBP2-74578AF700
Alexa Fluor 750 NBP2-74578AF750
Biotin NBP2-74578B
DyLight 350 NBP2-74578UV
DyLight 405 NBP2-74578V
DyLight 488 NBP2-74578G
DyLight 550 NBP2-74578R
DyLight 594 NBP2-74578DL594
DyLight 650 NBP2-74578C
DyLight 680 NBP2-74578FR
DyLight 755 NBP2-74578IR
FITC NBP2-74578F
HRP NBP2-74578H
Janelia Fluor 549 NBP2-74578JF549
Janelia Fluor 646 NBP2-74578JF646
Available Formulations
Formulation Catalog # Availability Price  
Azide and BSA Free NBP2-74578
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MAB1540

TRAILR2/TNFRSF10B Antibody (118929) [Unconjugated]

(2 Publications)
500 μg
MAB1540
TRAIL R2/TNFRSF10B was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Rat Anti-Mouse TRAIL R2/TNFRSF10B Monoclonal Antibody (Catalog # MAB1540) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter­stained with hematoxylin (blue). Specific staining was localized to cytoplasm of Purkinje neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Mu
Species Glossary
Reactivity: Mu
WB, IHC
Applications: WB, IHC
Rat Monoclonal
Host: Rat Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1540-SP
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10140-T2

Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

(3 Publications)
100 μg
10140-T2
Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera (Catalog #10140-T2) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TEC) mediated cytotoxicity using L‑929 mouse fibroblast cells. TheED50 for this effect is 0.75‑6 ng/mL.
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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NBP2-35030

Recombinant Human TRAILR2/TNFRSF10B Protein

10μg
NBP2-35030
SDS-Page: Human TRAIL R2/TNFRSF10B Protein [NBP2-35030]
Hu
Species Glossary
Reactivity: Hu
PAGE, Bioactivity
Applications: PAGE, Bioactivity
Sample Size Available
Sizes: Sample Size Available
Available Formulations
Formulation Catalog # Availability Price  
Carrier-Free NBP2-35030-1mg
Carrier-Free NBP2-35030-100ug
Carrier-Free NBP2-35030-500ug
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MAB6313

TRAILR2/TNFRSF10B Antibody (603307)

100 μg
MAB6313
Western blot shows lysates of HCT‑116 human colorectal carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TRAIL R2/TNFRSF10B Monoclonal Antibody (Catalog # MAB6313) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for TRAIL R2/TNFRSF10B at approximately 44 and 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB6313-SP
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721-DR

Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

(2 Publications)
100 μg
Mu
Species Glossary
Reactivity: Mu
Bioactivity
Applications: Bioactivity
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AFR10140

Recombinant Human TRAIL R2 Fc Alexa Fluor® 647 Protein

20 μg
AFR10140
Streptavidin coated beads conjugated to biotinylated anti-human TRAILR2 Antibody were stained with the indicated concentrations of Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Alexa Fluor® 647 (Catalog # AFR10140).
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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