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TRAILR2/TNFRSF10B Antibody (71903) [Unconjugated]

 (17 Publications)
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Human TRAIL R2/TNFRSF10B Monoclonal Antibody induces apoptosis of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner, as measured by Resazurin (Catalog # AR002) . The ED50 for this effect is typically 2‑12 ng/mL.
Human TRAIL R2/TNFRSF10B Monoclonal Antibody induces apoptosis of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner, as measured by Resazurin (Catalog # AR002) . The ED50 for this effect is ...read more
Cleavage of FRET probe is indicative of caspase activation.Cells were treated with (A) 0–300 ng/ml TRAIL for 2.5 hours or (B) 0–50 µg/ml anti-DR5 antibody for 20 h. The resultant cells were analyzed by western ...read more
MB231_CFP-YFP cells retain sensitivity to TRAIL and anti-DR5 antibody.(A) Parental MB231 (filled black) and MB231_CFP-YFP (open clear) cells were treated with TRAIL (0–100 ng/ml) for 2.5 h or anti-DR5 antibody ...read more
Cell-based FRET biosensor provides a quantifiable response to treatments of death receptor targeted cancer therapies.MB231_CFP-YFP cells were treated with (A) 0–300 ng/ml TRAIL for 2.5 h and (B) 0–100 µg/ml ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func
Clone
71903
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB631-SP
Carrier Free
MAB631-500
100% Guarantee on every product

TRAILR2/TNFRSF10B Antibody (71903) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL R2/TNFRSF10B
Met1-Glu182
Accession # CAG46696
Specificity
Detects human TRAIL R2/TNFRSF10B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R3, rhTRAIL R4, or rhDcR3.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF10B
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Agonist Activity 2-12 ng/mL
Publications
Read Publications using
MAB631 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAILR2/TNFRSF10B Antibody (71903) [Unconjugated]

  • CD262 antigen
  • CD262
  • death domain containing receptor for TRAIL/Apo-2L
  • Death receptor 5
  • DR5
  • DR5TRICK2B
  • Fas-like protein
  • KILLER/DR5
  • KILLERapoptosis inducing protein TRICK2A/2B
  • TNF-related apoptosis-inducing ligand receptor 2
  • TNFRSF10B
  • TRAIL R2
  • TRAIL receptor 2
  • TRAILR2
  • TRAIL-R2apoptosis inducing receptor TRAIL-R2
  • TRAILR2cytotoxic TRAIL receptor-2
  • TRICK2
  • TRICK2A
  • TRICKB
  • tumor necrosis factor receptor superfamily member 10B
  • tumor necrosis factor receptor superfamily, member 10b
  • tumor necrosis factor receptor-like protein ZTNFR9
  • ZTNFR9

Background

Human TRAIL R2, also called DR5 and TRICK 2 is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

  1. Chaudhary, P.M. et al. (1997) Immunity 7:821.
  2. Walczak, H. et al. (1997) EMBO J. 16:5386.
  3. Golstein, P. (1997) Curr. Biol. 7:R750.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRAILR2/TNFRSF10B Antibody (MAB631)(17)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Functional Assay, IHC-P.


Filter By Application
Functional Assay
(7)
IHC-P
(1)
All Applications
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Human
(8)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using MAB631 Applications Species
K.L. Phillips, N. Wright, E. McDermott, N.A. Cross TRAIL responses are enhanced by nuclear export inhibition in osteosarcoma Biochemical and Biophysical Research Communications 9/1/2019 [PMID: 31362889]
Asuka Onoe‑Takahashi, Manami Suzuki‑Karasaki, Miki Suzuki‑Karasaki, Toyoko Ochiai, Yoshihiro Suzuki‑Karasaki Autophagy inhibitors regulate TRAIL sensitivity in human malignant cells by targeting the mitochondrial network and calcium dynamics International Journal of Oncology 3/20/2019 [PMID: 30896851]
Liqiang Pan, Tian-Min Fu, Wenbin Zhao, Linlin Zhao, Wen Chen, Chixiao Qiu et al. Higher-Order Clustering of the Transmembrane Anchor of DR5 Drives Signaling Cell 3/1/2019 [PMID: 30827683]
Michael Boettcher, Sergio Covarrubias, Anne Biton, James Blau, Haopeng Wang, Noah Zaitlen et al. Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding BMC Genomics 12/1/2019 [PMID: 30727954]
Yohei Ohshima, Natsuhiko Takata, Miki Suzuki-Karasaki, Yukihiro Yoshida, Yasuaki Tokuhashi, Yoshihiro Suzuki-Karasaki Disrupting mitochondrial Ca2+ homeostasis causes tumor-selective TRAIL sensitization through mitochondrial network abnormalities International Journal of Oncology 10/1/2017 [PMID: 28849210]
Amr M. El Zawily, Behzad M. Toosi, Tanya Freywald, Vijaya V. Indukuri, Franco J. Vizeacoumar, Scot C. Leary et al. The intrinsically kinase-inactive EPHB6 receptor predisposes cancer cells to DR5-induced apoptosis by promoting mitochondrial fragmentation Oncotarget 11/22/2016 [PMID: 27788485]
Andrew K. Lewis, Christopher C. Valley, Stephen L. Peery, Benjamin Brummel, Anthony R. Braun, Christine B. Karim et al. Death Receptor 5 Networks Require Membrane Cholesterol for Proper Structure and Function Journal of Molecular Biology 12/1/2016 [PMID: 27720987]
Kang Z, Goldstein S, Yu Y, Meltzer P, Loeb D, Cao L Caspase-8 expression is predictive of tumour response to death receptor 5 agonist antibody in Ewing&#039;s sarcoma. Br J Cancer, 2015-08-20;113(6):894-901. 2015-08-20 [PMID: 26291055] (Functional Assay, Human) Functional Assay Human
Yoshihiro Suzuki-Karasaki, Kyoko Fujiwara, Kosuke Saito, Miki Suzuki-Karasaki, Toyoko Ochiai, Masayoshi Soma Distinct effects of TRAIL on the mitochondrial network in human cancer cells and normal cells: role of plasma membrane depolarization Oncotarget 5/25/2015 [PMID: 26057632]
Bozza , William, Di , Xu, Takeda , Kazuyo, Rivera Rosado , Leslie A, Pariser , Sarah, Zhang , Baolin The use of a stably expressed FRET biosensor for determining the potency of cancer drugs. PLoS ONE, 2014-09-04;9(9):e107010. 2014-09-04 [PMID: 25188024] (Functional Assay, Human) Functional Assay Human
Show All 17 Publications.

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Bioinformatics

Gene Symbol TNFRSF10B
Uniprot