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TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free

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Western Blot: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF10B.
Western Blot: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF10B.
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at ...read more
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Carcinoma of Human kidney tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C ...read more
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C ...read more
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Carcinoma of Human thyroid tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C ...read more
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Human tonsil tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, IHC
Clone
OTI2A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74578AF350
Alexa Fluor 405
NBP2-74578AF405
Alexa Fluor 488
NBP2-74578AF488
Alexa Fluor 532
NBP2-74578AF532
Alexa Fluor 594
NBP2-74578AF594
Alexa Fluor 647
NBP2-74578AF647
Alexa Fluor 700
NBP2-74578AF700
Alexa Fluor 750
NBP2-74578AF750
Biotin
NBP2-74578B
CoraFluor 1
NBP2-74578CL1
DyLight 350
NBP2-74578UV
DyLight 405
NBP2-74578V
DyLight 488
NBP2-74578G
DyLight 550
NBP2-74578R
DyLight 594
NBP2-74578DL594
DyLight 650
NBP2-74578C
DyLight 680
NBP2-74578FR
DyLight 755
NBP2-74578IR
FITC
NBP2-74578F
HRP
NBP2-74578H
Janelia Fluor 525
NBP2-74578JF525
Janelia Fluor 549
NBP2-74578JF549
Janelia Fluor 585
NBP2-74578JF585
Janelia Fluor 635
NBP2-74578JF635
Janelia Fluor 646
NBP2-74578JF646
Janelia Fluor 669
NBP2-74578JF669
Unconjugated
NBP2-46148
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46148
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TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 203-411 of human TNFRSF10B(NP_671716) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF10B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200-500
Theoretical MW
39.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free

  • CD262 antigen
  • CD262
  • death domain containing receptor for TRAIL/Apo-2L
  • Death receptor 5
  • DR5
  • DR5TRICK2B
  • Fas-like protein
  • KILLER/DR5
  • KILLERapoptosis inducing protein TRICK2A/2B
  • TNF-related apoptosis-inducing ligand receptor 2
  • TNFRSF10B
  • TRAIL R2
  • TRAIL receptor 2
  • TRAILR2
  • TRAIL-R2apoptosis inducing receptor TRAIL-R2
  • TRAILR2cytotoxic TRAIL receptor-2
  • TRICK2
  • TRICK2A
  • TRICKB
  • tumor necrosis factor receptor superfamily member 10B
  • tumor necrosis factor receptor superfamily, member 10b
  • tumor necrosis factor receptor-like protein ZTNFR9
  • ZTNFR9

Background

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently (1-2). Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TNFRSF10B