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![Western Blot: Histone H2B Antibody [NBP2-42854] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Western-Blot-NBP2-42854-img0008.jpg "Western Blot: Histone H2B Antibody [NBP2-42854] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Histone H2B Antibody [NBP2-42854] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: Histone H2B protein stained by Histone H2B antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42854-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H2B Antibody [NBP2-42854] - Analysis of mouse middle brain. Histone H2B antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunohistochemistry-Paraffin-NBP2-42854-img0002.jpg)
![Western Blot: Histone H2B Antibody [NBP2-42854] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Western-Blot-NBP2-42854-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H2B Antibody [NBP2-42854] - Analysis of H1299 xenograft, using Histone H2B antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunohistochemistry-Paraffin-NBP2-42854-img0001.jpg)
![Western Blot: Histone H2B Antibody [NBP2-42854] -The equivalent of 4 larvae was loadedA: 120hpf larvae4-12% NuPAGE Bis Tris precast gel diluted at 1:1000](http://images.novusbio.com/fullsize/antibody/nbp2-42854_rabbit-polyclonal-histone-h2b-antibody-western-blot-9820238527..jpg)
![Western Blot: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-1B3F12-A9-Western-Blot-NBP2-54770-img0001.jpg "Western Blot: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]")
![Chromatin Immunoprecipitation: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-1B3F12-A9-Chromatin-Immunoprecipitation-ChIP-NBP2-54770-img0004.jpg)
![ELISA: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-1B3F12-A9-ELISA-NBP2-54770-img0003.jpg)
![Western Blot: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK12ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Western-Blot-NBP2-59271-img0001.jpg "Western Blot: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK12ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - ChIP assays were performed using human HeLa cells, the antibody against H2BK12ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Chromatin-Immunoprecipitation-NBP2-59271-img0003.jpg)
![ELISA: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H2BK12ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:38,200.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-ELISA-NBP2-59271-img0002.jpg)
![Immunofluorescence: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - HeLa cells were stained with the antibody against H2BK12ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H2BK12ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunofluorescence-NBP2-59271-img0004.jpg)
![Western Blot: Histone H2B [ac Lys15] Antibody [NBP2-59272] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK15ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Western-Blot-NBP2-59272-img0001.jpg "Western Blot: Histone H2B [ac Lys15] Antibody [NBP2-59272] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK15ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H2B [ac Lys15] Antibody [NBP2-59272] - HeLa cells were stained with the antibody against H2BK15ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/ TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H2BK15ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunofluorescence-NBP2-59272-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H2B [ac Lys15] Antibody [NBP2-59272] - ChIP assays were performed using human HeLa cells, the antibody against H2BK15ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Chromatin-Immunoprecipitation-NBP2-59272-img0003.jpg)
![ELISA: Histone H2B [ac Lys15] Antibody [NBP2-59272] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H2BK15ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:29,700.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-ELISA-NBP2-59272-img0002.jpg)
![Western Blot: Histone H2B Antibody [NBP2-59270] - Western blot was performed on whole cell extracts from HeLa cells (40 ug, lane 1) and on 1 ug of recombinant histone H2B (lane 2) using the antibody against H2B. The antibody was diluted 1:10,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Western-Blot-NBP2-59270-img0002.jpg "Western Blot: Histone H2B Antibody [NBP2-59270] - Western blot was performed on whole cell extracts from HeLa cells (40 ug, lane 1) and on 1 ug of recombinant histone H2B (lane 2) using the antibody against H2B. The antibody was diluted 1:10,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H2B Antibody [NBP2-59270] - HeLa cells were stained with the antibody against H2B and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the H2B antibody (middle) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59270-img0001.jpg)
![Chromatin Immunoprecipitation: Histone H2B Antibody [NBP2-59270] - ChIP assays were performed using human HeLa cells, the antibody against H2B and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as negative controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Chromatin-Immunoprecipitation-NBP2-59270-img0004.jpg)
![ELISA: Histone H2B Antibody [NBP2-59270] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H2B in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-ELISA-NBP2-59270-img0003.jpg)
![Western Blot: Histone H2B Antibody (3U2P5) [NBP3-15598] - Western blot analysis of extracts of various cell lines, using Histone H2B antibody (NBP3-15598) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-3U2P5-Western-Blot-NBP3-15598-img0004.jpg "Western Blot: Histone H2B Antibody (3U2P5) [NBP3-15598] - Western blot analysis of extracts of various cell lines, using Histone H2B antibody (NBP3-15598) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Histone H2B Antibody (3U2P5) [NBP3-15598] - Immunohistochemistry of paraffin-embedded mouse kidney using Histone H2B Rabbit mAb (NBP3-15598) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-3U2P5-Immunohistochemistry-Paraffin-NBP3-15598-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H2B Antibody (3U2P5) [NBP3-15598] - Immunohistochemistry of paraffin-embedded rat brain using Histone H2B Rabbit mAb (NBP3-15598) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-3U2P5-Immunohistochemistry-Paraffin-NBP3-15598-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H2B Antibody (3U2P5) [NBP3-15598] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Histone H2B Rabbit mAb (NBP3-15598) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-3U2P5-Immunohistochemistry-Paraffin-NBP3-15598-img0002.jpg)
![Western Blot: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - Analysis on whole cell extracts from WT yeast cells (lane 1), yeast cells with higher steady state levels of H2BK123ub (lane 2), none ubiquitinylated H2B (lane 3) and ubiquitinylated H2A (lane 4). The position of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-C-15200232-Western-Blot-NBP2-59149-img0002.jpg "Western Blot: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - Analysis on whole cell extracts from WT yeast cells (lane 1), yeast cells with higher steady state levels of H2BK123ub (lane 2), none ubiquitinylated H2B (lane 3) and ubiquitinylated H2A (lane 4). The position of the protein of interest is indicated on the right.")
![Chromatin Immunoprecipitation: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - ChIP assays were performed using human HeLa cells, the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive TSH2B gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-C-15200232-Chromatin-Immunoprecipitation-NBP2-59149-img0003.jpg)
![Western Blot: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - Analysis on histone extracts from HeLa cells (15 ug) using the antibody against H2BK123ub. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-C-15200232-Western-Blot-NBP2-59149-img0001.jpg)
![Chromatin Immunoprecipitation: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - ChIP results obtained with the antibody against H2BK123ub on sheared chromatin from WT yeast cells, yeast cells with higher steady state levels of H2BK123ub and yeast cells with no ubiquitinylated H2B. Quantitative PCR was performed with primers for the coding region of an active gene. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-C-15200232-Chromatin-Immunoprecipitation-NBP2-59149-img0004.jpg)
![Western Blot: Recombinant Human Histone H2B Protein [NBP3-07913] - Lane 1: MWM; Lane 2: 1.0 ug Human Histone H2B Protein.](http://images.novusbio.com/fullsize/Recombinant-Human-Histone-H2B-Protein-Western-Blot-NBP3-07913-img0002.jpg "Western Blot: Recombinant Human Histone H2B Protein [NBP3-07913] - Lane 1: MWM; Lane 2: 1.0 ug Human Histone H2B Protein.")
![Western Blot: Recombinant Human Histone H2B Protein [NBP3-07913] - Lane 1: MWM; Lane 2: 100 ng Human Histone H2B Protein probed with Histone H2B pAb.](http://images.novusbio.com/fullsize/Recombinant-Human-Histone-H2B-Protein-Western-Blot-NBP3-07913-img0001.jpg)
![Western Blot: Histone H2B Antibody (RM230) [NBP2-61495] - Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using NBP2-61495 at 0.2 ug/ml](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-RM230-Western-Blot-NBP2-61495-img0003.jpg "Western Blot: Histone H2B Antibody (RM230) [NBP2-61495] - Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using NBP2-61495 at 0.2 ug/ml")
![Immunocytochemistry/Immunofluorescence: Histone H2B Antibody (RM230) [NBP2-61495] - Immunocytochemical staining of HeLa cells, using NBP2-61495 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-RM230-Immunocytochemistry-Immunofluorescence-NBP2-61495-img0002.jpg)
![Western Blot: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B (acetyl Lys20) antibody [GT578] (NBP3-13538) diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H2B-ac-Lys20-Antibody-GT578-Western-Blot-NBP3-13538-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B (acetyl Lys20) antibody [GT578] (NBP3-13538) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] - Histone H2B (acetyl Lys20) antibody [GT578] detects Histone H2B (acetyl Lys20) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2B (acetyl Lys20) stained by Histone H2B (acetyl Lys20) antibody [GT578] (NBP3-13538) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Histone-H2B-ac-Lys20-Antibody-GT578-Immunocytochemistry-Immunofluorescence-NBP3-13538-img0002.jpg)
![Western Blot: Histone H2B Overexpression Lysate (Adult Normal) [NBP2-11038] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H2B.](http://images.novusbio.com/fullsize/Histone-H2B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11038-img0001.jpg "Western Blot: Histone H2B Overexpression Lysate (Adult Normal) [NBP2-11038] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H2B.")
![Western Blot: H2B Overexpression Lysate (Adult Normal) [NBL1-11571] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for H2B.](http://images.novusbio.com/fullsize/H2B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11571-img0002.jpg "Western Blot: H2B Overexpression Lysate (Adult Normal) [NBL1-11571] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for H2B.")