Hu,
Mu,
Rt,
Ca,
Fe,
PoSpecies Glossary
Reactivity: Hu, Mu, Rt, Ca, Fe, Po
WB, ELISA, ICC/IF, IHC, Dual ISH-IHC, IHC, KO
Applications: WB, ELISA, ICC/IF, IHC, Dual ISH-IHC, IHC, KO
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
![ADT induced SPINK1 upregulation associates with NE-phenotype in mice and NEPC patients.a Box plots depicting relative expression of SPINK1, SYP, CHGA, TUBB3, and VIM transcripts (read counts) in VCaP tumors implanted orthotopically in orchiectomized mice and subjected to vehicle (n = 4) or anti-androgens [enzalutamide (n = 4) or ARN-509 (n = 4)] treatment for 4 weeks (GSE95413). b Representative images of immunohistochemical staining for the same markers shown in a using VCaP xenograft tumors as described in a. Scale bar represents 100 μm. c Box plots depicting quantification of the immunohistochemical staining in VCaP xenografts for the markers shown in b. d Representative images for immunohistochemical staining of SPINK1, SYP, CHGA, and TUBB3 in 22RV1 xenograft tumors excised from orchiectomized mice treated with enzalutamide (20 mg/kg body weight) or vehicle control (n = 5 each). Intact group represents non-castrated control mice (n = 5). Scale bar represents 50 μm. e Box plots depicting quantification of the immunohistochemical staining in 22RV1 xenografts for the markers shown in d. f Representative images showing H&E staining (×200 magnification) and immunostaining (×200 magnification) for AR, synaptophysin, and SPINK1 in tumor specimens obtained from NEPC patients’, namely WCM12, WCM155 (an organoid), and WCM677. Scale bar represents 100 µm. Data are presented as box-and-whisker plots with median, where the box extends from 25th–75th percentile, and whiskers ranges from minimum and maximum values. For panels a, c, e one-way ANOVA, Dunnett’s multiple-comparisons test was applied. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1028_goat-polyclonal-aif-1-iba1-antibody-2322024894214.jpg)
![TRF2 transcriptionally regulates p21 expression through promoter occupancy. (A) Quantitative ChIP using TRF2 antibody gives enriched binding of TRF2 on p21 promoter in HT1080 and MDA-MB-231 cells, IgG was used as isotypic antibody control; normalized with 10% input (data represented as mean ± SEM, for three replicates). (B,C) TRF2 represses p21 promoter activity. In luciferase assay, si-RNA-mediated silencing or TRF2 over expression resulted in increased (B, *p value < 0.05, Student’s t-test; data represented as mean ± SEM of three replicates) or reduction (C, *p value < 0.05, Student’s t-test; data represented as mean ± SEM of three replicates) in p21 promoter activity in HT1080 and MDA-MB-231 cells, respectively; over expression of TRF2 devoid of DNA binding [deletion of basic (delB), myb (delM) and both basic/myb (delB-delM) domains] resulted in partial or complete rescue of p21 promoter activity in HT1080 and MDA-MB-231 cells (data represented as mean ± SEM, for three replicates) (C). (D,E) HT1080 cells over expressing TRF2 show reduced p21 protein expression, bar graph shows the densitometry analysis of three different immunoblot replicates (*p value < 0.05, Student’s t-test; data represented as mean ± SEM of three replicates). (E) (Full images are shown in Supplementary Figure S5); TRF2 silencing results in increase in p21 protein, bar graph shows the densitometry analysis of three different immunoblots (*p value < 0.05, Student’s t-test; data represented as mean ± SEM of three replicates) (F); and mRNA expression in HT1080 cells (*p value < 0.05, Student’s t-test; data represented as mean ± SEM of three replicates, GAPDH used as internal control for real-time PCR). Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41598-017-11177-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1028_goat-polyclonal-aif-1-iba1-antibody-23220248194396.jpg)
![Western Blot: AIF-1/Iba1 Antibody [NBP2-19019] - Various 50 ug tissue extracts (Molecular weight: 16.7 KDa) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 Antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Western-Blot-NBP2-19019-img0014.jpg "Western Blot: AIF-1/Iba1 Antibody [NBP2-19019] - Various 50 ug tissue extracts (Molecular weight: 16.7 KDa) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 Antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: AIF-1/Iba1 stained by AIF-1/Iba1 Antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19019-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Mouse fore brain. Iba1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-19019-img0020.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-19019] - THP-1 cell. Black: Unlabelled sample was used as a control.Red: AIF-1:Iba1 Antibody dilution: 1:50.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP2-19019-img0001.jpg)
![Western Blot: AIF-1/Iba1 Antibody [NBP2-19019] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 Antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Western-Blot-NBP2-19019-img0012.jpg)
![Western Blot: AIF-1/Iba1 Antibody [NBP2-19019] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 Antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Western-Blot-NBP2-19019-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] - AIF-1/Iba1 Antibody (1 1:1000, Red) expression in dorsal root ganglia. Green fluorescent Nissl was used as counter-staining. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19019-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AIF-1/Iba1 protein stained by AIF-1/Iba1 Antibody diluted at 1:400. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19019-img0003.jpg)
![Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NBP2-19019] - Frozen sectioned E13.5 Rat brain. Green: AIF-1/Iba1 protein stained by AIF-1/Iba1 Antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Frozen-NBP2-19019-img0005.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Mouse cerebellum stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-19019-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Rat brain stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-19019-img0010.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Rat cerebellum stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-19019-img0011.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Rat hind brain. Iba1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-19019-img0016.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Analysis of paraffin-embedded human hepatoma, using at 1:500 dilution.Iba1 antibody detects AIF-1/Iba1 in the cytosol of Kupffer cell in human hepatoma by immunohistochemical analysis.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-19019-img0018.jpg)
![Immunohistochemistry: AIF-1/Iba1 Antibody [NBP2-19019] - LCN2 mainly expressed on neurons. a Representative immunofluorescence images of LCN2 (green) and AIF-1/Iba1 (red) staining in sections of the hippocampus after surgery. Long scale bar, 600 um; short scale bar, 25 um. Image collected and cropped by CiteAb from the following publication (pubmed.ncbi.nlm.nih.gov/35413913) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-19019_rabbit-polyclonal-aif-1-iba1-antibody-2552023153956.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2833-img0015.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-2833] - Staining (6ug/ml) of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NB100-2833-img0016.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NB100-2833] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NB100-2833-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Pg/gingipain is present intracellularly in astrocytes, microglia and neurons in the hippocampus of experimental mice.Green: astrocytes (top panels), microglia (middle panels), and neurons (bottom panels). Red: Pg/gingipain. Blue: DAPI. Representative of N = 4 (GFAP), 3 (Iba1) and 4 (NeuN) experimental mice. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0204941), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2833-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2833-img0014.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-2833] - Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NB100-2833-img0017.jpg)
![Immunoprecipitation: AIF-1/Iba1 Antibody [NBP2-16908] - Immunoprecipitation of AIF-1/Iba1 protein from K562 whole cell extracts using 5ug of AIF-1/Iba1 Antibody Western blot analysis was performed using Iba1 antibody EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunoprecipitation-NBP2-16908-img0018.jpg "Immunoprecipitation: AIF-1/Iba1 Antibody [NBP2-16908] - Immunoprecipitation of AIF-1/Iba1 protein from K562 whole cell extracts using 5ug of AIF-1/Iba1 Antibody Western blot analysis was performed using Iba1 antibody EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Western-Blot-NBP2-16908-img0045.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16908-img0041.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Rat brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0039.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: AIF-1:Iba1 Antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP2-16908-img0032.jpg)
![Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Western-Blot-NBP2-16908-img0043.jpg)
![Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NBP2-16908] - Frozen sectioned E13.5 Rat brain. Green: AIF-1/Iba1 protein stained by AIF-1/Iba1 Antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Frozen-NBP2-16908-img0027.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Rat brain. Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0029.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse cerebellum. Iba1 stained by Iba1 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0033.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Rat cerebellum. Iba1 stained by Iba1 antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0034.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0035.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0036.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Rat brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0040.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0038.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - Primary murine microglia cells, staining with AIF-1:Iba1 Antibody using 1. 0 ug per 4 x 10^5 cells. NBP2-16908 (blue), Rabbit IgG (green) Unstained (red).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP2-16908-img0014.jpg)
![Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Western-Blot-NBP3-13648-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat brain. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0008.jpg)
![Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Western-Blot-NBP3-13648-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse spinal cord. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:200. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Mouse hippocampus. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat cerebellum. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:150. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0004.jpg)
![Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Red: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22-RT] diluted at 1:100. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0005.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Mouse brain. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody [HL22] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunocytochemistry-immunofluorescence-244202411033..jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat brain.Iba1 stained by Iba1 antibody [HL22] conjugated with DyLight488 diluted at 1:200.Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunohistochemistry-paraffin-244202411220..jpg)
![Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Frozen-sectioned mouse spinal cord.Green: Iba1 stained by Iba1 antibody [HL22] diluted at 1:100.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunohistochemistry-frozen-244202411414..jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Analysis in human spleen and skeletal muscle tissues using . Corresponding AIF-1/Iba1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0001.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Analysis in human spleen and skeletal muscle tissues using . Corresponding AIF-1/Iba1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human spleen using AIF-1/Iba1 Antibody shows very strong membranous positivity in cells in red pulp.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human cerebral cortex using AIF-1/Iba1 Antibody shows strong membranous positivity in glial cells.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human kidney using AIF-1/Iba1 Antibody shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human lymphoid tissues using AIF-1/Iba1 Antibody shows very strong membranous positivity in non-germinal, and germinal center cells.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human placenta using AIF-1/Iba1 Antibody shows very strong cytoplasmic positivity in Hofbauer cells.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human skeletal muscle using AIF-1/Iba1 Antibody shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP1-06014] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using AIF-1/Iba1 Antibody (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06014-img0009.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP1-06014] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using AIF-1/Iba1 Antibody (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP1-06014] - Staining of paraffin embedded Human Cerebellum AIF-1/Iba1 Antibody at 4 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP1-06014-img0007.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP1-06014] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) with AIF-1:Iba1 Antibody, followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP1-06014-img0008.jpg)
![Western Blot: AIF-1/Iba1 Antibody (SR1261) [NBP3-21860] - Western blot analysis using NBP3-21860 in U937 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21860_rabbit-aif-1-iba1-mab-sr1261-5720231512345.jpg "Western Blot: AIF-1/Iba1 Antibody (SR1261) [NBP3-21860] - Western blot analysis using NBP3-21860 in U937 cell lysate.")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (rAIF1/1909) [NBP2-75760] - Formalin-fixed, paraffin-embedded human Tonsil stained with Mouse Recombinant AIF-1/Iba1 Antibody (rAIF1/1909) - Azide and BSA Free.](http://images.novusbio.com/fullsize2/AIF-1-Iba1-Antibody-(rAIF1-1909)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-75760-img0001.jpg "Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (rAIF1/1909) [NBP2-75760] - Formalin-fixed, paraffin-embedded human Tonsil stained with Mouse Recombinant AIF-1/Iba1 Antibody (rAIF1/1909) - Azide and BSA Free.")
![Protein Array: AIF-1/Iba1 Antibody (rAIF1/1909) [NBP2-75760] - Analysis of Protein Array containing >19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize2/AIF-1-Iba1-Antibody-(rAIF1-1909)---Azide-and-BSA-Free-Protein-Array-NBP2-75760-img0002.jpg)