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![Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (RA3-6B2) - Chimeric [NBP3-11980] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of RA3-6B2 (NBP3-11980) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11980, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-RA3-6B2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11980-img0001.jpg "Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (RA3-6B2) - Chimeric [NBP3-11980] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of RA3-6B2 (NBP3-11980) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11980, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: B220/CD45R Antibody (RA3-6B2) - Chimeric [NBP3-11980] - Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of RA3-6B2 (NBP3-11980, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-RA3-6B2-Chimeric-Flow-Cytometry-NBP3-11980-img0002.jpg)
![B220/CD45R Antibody (RA3-6B2) [mFluor Violet 610 SE] [NBP3-11980MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.](http://images.novusbio.com/fullsize/nbp3-11980mfv610_rabbit-b220-cd45r-mab-ra3-6b2-mfluor-violet-610-se-672023914745.png "B220/CD45R Antibody (RA3-6B2) [mFluor Violet 610 SE] [NBP3-11980MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.")