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NBP2-24729

TLR9 Antibody (26C593.2) - BSA Free

(4 Reviews)

(152 Publications)

0.1 mg

NBP2-24729

Simple Western: TLR9 Antibody (26C593.2) [NBP2-24729] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. Image using the Azide Free format of this antibody.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 protein on epithelial cells. HNEC, Detroit-562 and FaDu were stained intracellularly with PE-Ab against TLR9 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0098239), licensed under a CC-BY license.

<b>Genetic Strategies Validation. </b>Knockdown Validated: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092748) licensed under a CC-BY license.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of colonic LP or peritoneal cavity (PerC) of normal mice were evaluated by flow cytometry. B cells were intracellularly stained with the anti-TLR9 antibody after the cell surface staining with anti-B220 and CD5 antibodies, and examined using flow cytometry. N = 3, performed twice. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0146191) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: TLR9 Antibody (26C593.2) [NBP2-24729] - Cbl-b is required for the endocytic transit of TLR9. Representative confocal microscopic images of splenocytes from mice with indicated genotypes. For experiments, cells were stimulated through the BCR (green) for 30 minutes then fixed and stained for TLR9 (blue) and Lamp-1 (red)(n = 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0089792) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) [NBP2-24729] - Monkey retina tissue. Image from verified customer review.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Gating strategy for the detection of TLR-7 and -9 in B cell subsets. PBMC were isolated from whole blood and stained for surface markers before cells were fixed, permeabilised and stained for TLR-9. FSC and SSC were first used to gate out debris and SSC-A and SSC-H was utilized to eliminate duplicates. Further gating was done on CD45 and CD19, to target B cells. To separate between the different B cell populations, we gated on CD27 and IgD (D), followed by TLR-9 expression on these subsets. Data from one representative patient is shown. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0120383) licensed under a CC-BY license.

Western Blot: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using this antibody at a dilution of 3 ug/ml.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 on turbinate epithelial cells from healthy controls compared to turbinate and polyp epithelial cells from patients with CRSwNP, n = 5 (A). Intracellular staining for TLR9 (open histogram, black line) and isotype control (filled histogram) on turbinate epithelial cells from a healthy control (B), turbinate epithelial cells (C) and polyp epithelial cells from a patient (D), analysed using flow cytometry. Results are presented as mean +/- SEM, **P<0.01. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0105618) licensed under a CC-BY license.

TLR9 activation upregulated PD-L1 expression by promoting STAT3 Tyr705 phosphorylation in HCC cells. (A) PD-L1 protein expression after treatment with ODN2216 with different concentrations or the indicated times (different concentrations: 0, 5, 10, or 20uM; times: 0, 6, 12, 24, and 36 hours in 10 uM) in Hep3B cells. PD-L1 protein levels were analyzed by Western blotting. (B) PD-L1 protein expression after treatment with ODN2216 with different concentrations or the indicated times (different concentrations: 0, 2.5, 5, or 10uM; times: 0, 6, 12, 24, and 36 hours in 10 uM) in Huh7 cells. PD-L1 protein levels were analyzed by Western blotting. (C) PD-L1 protein expression after treatment with ODN1585 with different concentrations or the indicated times (different concentrations: 0, 2.5, 5, and 10 uM; times: 0, 6, 12, 24, and 36 hours in 5uM) in Hepa1-6 cells. PD-L1 protein levels were analyzed by Western blotting. (D) PD-L1+ tumor cells were detected by flow cytometry after TLR9 agonist (Hep3B and Huh7 cells with ODN2216; Hepa1-6 cells with ODN1585) treatment with indicated concentration. (values are mean +/- SD, *p < 0.05, **p < 0.01, ***p<0.001, NS indicates no significance). (E) PD-L1 expression after TLR9 overexpression in Huh7 cells. PD-L1 expression levels were analyzed by immunofluorescence. (F) PD-L1 protein expression after TLR9 overexpression in Huh7 cells. PD-L1 protein levels were analyzed by Western blotting. (G) PD-L1 protein expression in TLR9 knockdown or TLR9 rescue Hep3B cells. PD-L1 protein levels were analyzed by Western blotting. (H and I) mRNA levels of PD-L1 in Hep3B (H) and Huh7 (I) cells measured by qRT-PCR after stimulation with different concentrations of ODN2216. (values are mean +/- SD, *p < 0.05, **p < 0.01, NS indicates no significance) (J) TLR9 overexpression-induced PD-L1 expression after MYC, JUN, IRF1, IRF3, STAT1 or STAT3 silencing. PD-L1 mRNA expression in Huh7 cells was analyzed after TLR9 overexpression alone or in the presence of MYC-, JUN-, IRF1-, IRF3-, STAT1- or STAT3-specific siRNA or siRNA-NC. (values are mean +/- SD, ***p<0.001). (K) p-STAT3 (Tyr705) levels in Hep3B cells after treatment with different concentrations of ODN2216 (a TLR9 agonist; ODN2216: 0, 2.5, 5, or 10uM) analyzed by Western blotting. (L) p-STAT3 (Tyr705) levels after TLR9 overexpression in Huh7 cells analyzed by Western blotting. (M) TLR9 overexpression-induced p-STAT3 (Tyr705) levels after TLR9 inhibition. p-STAT3 (Tyr705) levels were analyzed after TLR9 overexpression alone or in the presence of the TLR9 antagonist chloroquine diphosphate. (N) p-STAT3-induced PD-L1 levels after STAT3 inhibition. PD-L1 levels were analyzed after TLR9 overexpression alone or in the presence of the STAT3-specific small molecular inhibitor BP-1-102. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483468), licensed under a CC-BY licence.

THP-1 human acute monocytic leukemia cell line was stained with Mouse anti-TLR9 (26C593.2) Protein-G purified Monoclonal Antibody conjugated to Janelia Fluor® 549 (Catalog # NBP2-24729JF549, blue histogram) or matched control antibody (orange histogram).

Reactivity:

Hu, Mu, Rt, Ca, Eq, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Eq, Pm, Pm

Applications:

, , , , , , , , , , ,

Applications: , , , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-305A

Alt Catalog# IMG-305A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24729AF350
Alexa Fluor 405	NBP2-24729AF405
Alexa Fluor 488	NBP2-24729AF488
Alexa Fluor 532	NBP2-24729AF532
Alexa Fluor 594	NBP2-24729AF594
Alexa Fluor 647	NBP2-24729AF647
Alexa Fluor 700	NBP2-24729AF700
Alexa Fluor 750	NBP2-24729AF750
Allophycocyanin	NBP2-24729APC
Biotin	NBP2-24729B
Biotin	NBP2-24890
DyLight 350	NBP2-24729UV
DyLight 405	NBP2-24729V
DyLight 488	NBP2-24729G
DyLight 550	NBP2-24729R
DyLight 594	NBP2-24729DL594
DyLight 650	NBP2-24729C
DyLight 680	NBP2-24729FR
DyLight 755	NBP2-24729IR
FITC	NBP2-24729F
HRP	NBP2-24729H
Janelia Fluor 549	NBP2-24729JF549
Janelia Fluor 646	NBP2-24729JF646
PE	NBP2-24907
PE	NBP2-24729PE
PerCP	NBP2-24729PCP
mFluor Violet 450 SE	NBP2-24729MFV450
mFluor Violet 500 SE	NBP2-24729MFV500
mFluor Violet 610 SE	NBP2-24729MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide Free	NBP2-24863
BSA Free	NBP2-24729SS

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AF3658

TLR9 Antibody

(1 Publication)

100 μg

AF3658

Human PBMC lymphocytes were stained with Sheep Anti-Human TLR9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3658) followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).

TLR9 was detected in immersion fixed paraffin-embedded sections of human tonsil using Sheep Anti-Human TLR9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3658) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to membranes of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	IC7108G

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC7108G
Carrier Free	AF3658-SP

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MAB3658

TLR9 Antibody (229106)

(1 Publication)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC3658U-100UG
Alexa Fluor 405	IC3658V-100UG
Alexa Fluor 488	IC3658G-100UG
Alexa Fluor 594	IC3658T-100UG
Alexa Fluor 647	IC3658R-100UG
Alexa Fluor 700	IC3658N-100UG
Alexa Fluor 750	IC3658S-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3658-SP
Carrier Free	IC3658V-100UG
Carrier Free	IC3658U-100UG
Carrier Free	IC3658T-100UG
Carrier Free	IC3658S-100UG
Carrier Free	IC3658R-100UG
Carrier Free	IC3658N-100UG
Carrier Free	IC3658G-100UG

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MAB36582

TLR9 Antibody (980914) [Unconjugated]

100 μg

MAB36582

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and (A) Mouse Anti-Human/Mouse TLR9 Monoclonal Antibody (Catalog # MAB36582) or (B) Mouse IgG1 control antibody (Catalog # MAB002) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC36582U-100UG
Alexa Fluor 350	FAB36582U
Alexa Fluor 405	IC36582V-100UG
Alexa Fluor 405	FAB36582V
Alexa Fluor 488	IC36582G-100UG
Alexa Fluor 488	FAB36582G
Alexa Fluor 532	FAB36582X
Alexa Fluor 594	IC36582T-100UG
Alexa Fluor 594	FAB36582T
Alexa Fluor 647	FAB36582R
Alexa Fluor 647	IC36582R-100UG
Alexa Fluor 700	FAB36582N
Alexa Fluor 700	IC36582N-100UG
Alexa Fluor 750	FAB36582S
Alexa Fluor 750	IC36582S-100UG
Allophycocyanin	FAB36582A
Biotin	FAB36582B
DyLight 350	FAB36582D
DyLight 405	FAB36582E
DyLight 488	FAB36582K
DyLight 550	FAB36582L
DyLight 594	FAB36582M
DyLight 650	FAB36582W
DyLight 680	FAB36582Y
DyLight 755	FAB36582Z
FITC	FAB36582F
HRP	FAB36582H
Janelia Fluor 549	FAB36582I
Janelia Fluor 646	FAB36582J
PE	FAB36582P
PerCP	FAB36582C
mFluor Violet 450 SE	FAB36582MFV450
mFluor Violet 500 SE	FAB36582MFV500
mFluor Violet 610 SE	FAB36582MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB36582-SP
Carrier Free	IC36582S-100UG
Carrier Free	IC36582V-100UG
Carrier Free	IC36582U-100UG
Carrier Free	IC36582R-100UG
Carrier Free	IC36582T-100UG
Carrier Free	IC36582N-100UG
Carrier Free	IC36582G-100UG

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MAB36583

TLR9 Antibody (26C593R) [Unconjugated]

100 μg

MAB36583

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and (A) Mouse Anti-Human TLR9 Monoclonal Antibody (Catalog # MAB36583) or (B) Mouse IgG1 control antibody (Catalog # MAB002) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC36583U-100UG
Alexa Fluor 350	FAB36583U
Alexa Fluor 405	IC36583V-100UG
Alexa Fluor 405	FAB36583V
Alexa Fluor 488	IC36583G-100UG
Alexa Fluor 488	FAB36583G
Alexa Fluor 532	FAB36583X
Alexa Fluor 594	IC36583T-100UG
Alexa Fluor 594	FAB36583T
Alexa Fluor 647	IC36583R-100UG
Alexa Fluor 647	FAB36583R
Alexa Fluor 700	IC36583N-100UG
Alexa Fluor 700	FAB36583N
Alexa Fluor 750	FAB36583S
Alexa Fluor 750	IC36583S-100UG
Allophycocyanin	FAB36583A
Biotin	FAB36583B
DyLight 350	FAB36583D
DyLight 405	FAB36583E
DyLight 488	FAB36583K
DyLight 550	FAB36583L
DyLight 594	FAB36583M
DyLight 650	FAB36583W
DyLight 680	FAB36583Y
DyLight 755	FAB36583Z
FITC	FAB36583F
HRP	FAB36583H
Janelia Fluor 549	FAB36583I
Janelia Fluor 646	FAB36583J
PE	FAB36583P
PerCP	FAB36583C
mFluor Violet 450 SE	FAB36583MFV450
mFluor Violet 500 SE	FAB36583MFV500
mFluor Violet 610 SE	FAB36583MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB36583-SP
Carrier Free	IC36583V-100UG
Carrier Free	IC36583U-100UG
Carrier Free	IC36583T-100UG
Carrier Free	IC36583G-100UG
Carrier Free	IC36583R-100UG
Carrier Free	IC36583S-100UG
Carrier Free	IC36583N-100UG

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