| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-122SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76657-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72598 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76610-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71366 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72925 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77196-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74612 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70254 | |||
![<b>Genetic Strategies Validation. </b>Reduction of HIF-2alpha levels leads to protection in UV-triggered apoptosis, but not for apoptosis caused by glucose and serum starvation in 786-O cells. Parental 786-O or those either stably expressing wild-type VHLp19 or stably infected with a control vector (pSuperRetro) or a pool of two HIF-2alpha shRNAs vectors [21] were grown to confluence and lysed. Cell alpha-tubulin.](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Knockdown-Validated-NB100-122-img0033.jpg)
![Effect of the four most common herbal formulas and single herbs on the phosphorylation of myosin light chain (MLC) protein.Briefly, A10 cells were treated with herbal formulas (A) or single herbs (B). Y27632 (Y10; 10 μM) and calyculin A (A50; 50 μg/ml) were used as negative and positive controls. Western blot analysis and staining with anti-phospho-MLC, anti-total-MLC, and anti-beta actin antibodies was then performed. Phospho-MLC, total-MLC, and beta actin were all obtained with their appropriate protein size bands. The relative Phospho-MLC intensity (%) was expressed as [(Phospho-MLC/total-MLC)drug treated/ (Phospho-MLC/total-MLC)cell only x 100%]. The Mean±SEM values for at least three independent experiments along with the representative western blot were performed. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0145109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-122_rabbit-polyclonal-hif-2-alpha-epas1-antibody-2322024820301.jpg)
![Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-19667-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-19667] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using p53 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19667-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NBP2-19667-img0007.jpg)
![Whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-19667_rabbit-polyclonal-p53-antibody-western-blot-22320239226.jpg)
![Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human Endo G recombinant protein per lane. Antibodies: Endo G NBP1-76657, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL Lane 2: 1 ug/mL Lane 3: 2 ug/mL](http://images.novusbio.com/fullsize/Endo-G-Antibody-Western-Blot-NBP1-76657-img0010.jpg "Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human Endo G recombinant protein per lane. Antibodies: Endo G NBP1-76657, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL Lane 2: 1 ug/mL Lane 3: 2 ug/mL")
![Immunohistochemistry: Endo G Antibody [NBP1-76657] - Analysis of human pancreas tissue with EndoG antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Endo-G-Antibody-Immunohistochemistry-NBP1-76657-img0005.jpg)
![Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation in Mouse 3T3 (M) and Human HepG2 (H) cell lysates. Loading: 15 ug of lysates per lane. Antibodies: Endo G NBP1-76657 (2 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Endo-G-Antibody-Western-Blot-NBP1-76657-img0008.jpg)
![Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation in Human A431 Cell Lysate with the presence (A) or absence (B and C) of blocking peptide. Loading: 15 ug of lysates per lane. Antibodies: Endo G NBP1-76657 (A: 0.5 ug/mL, B: 0.5 ug/mL, C: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Endo-G-Antibody-Western-Blot-NBP1-76657-img0009.jpg)
![Immunohistochemistry-Paraffin: Endo G Antibody [NBP1-76657] - Human pancreas with anti-Endo G at 15 ug/ml.](http://images.novusbio.com/fullsize/Endo-G-Antibody-Immunohistochemistry-Paraffin-NBP1-76657-img0001.jpg)
![Immunofluorescence: Endo G Antibody [NBP1-76657] - Human pancreas tissue with EndoG antibody at 20 ug/mL. Green: EndoG Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/Endo-G-Antibody-Immunofluorescence-NBP1-76657-img0007.jpg)
![Western Blot: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL-1.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Western-Blot-NBP2-45773-img0009.jpg "Western Blot: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL-1.")
![Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of Carcinoma of prostate tissue.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Immunohistochemistry-NBP2-45773-img0008.jpg)
![Flow Cytometry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of HEK293T cells transfected with either pCMV6-ENTRY MCL1.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Flow-Cytometry-NBP2-45773-img0001.jpg)
![Western Blot: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of extracts (10ug).](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Western-Blot-NBP2-45773-img0010.jpg)
![Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of breast tissue.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Immunohistochemistry-NBP2-45773-img0003.jpg)
![Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of Kidney tissue within the normal limits.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Immunohistochemistry-NBP2-45773-img0004.jpg)
![Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of Carcinoma of liver tissue.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Immunohistochemistry-NBP2-45773-img0005.jpg)
![Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of Carcinoma of pancreas tissue.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Immunohistochemistry-NBP2-45773-img0006.jpg)
![Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of prostate tissue.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Immunohistochemistry-NBP2-45773-img0007.jpg)
![Immunofluorescence: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MCL-1.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-OTI10F6-Immunofluorescence-NBP2-45773-img0002.jpg)
![Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP1-76610-img0006.jpg "Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76610-img0003.jpg)
![Immunohistochemistry: Caspase-8 Antibody [NBP1-76610] - Staining of human spleen tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunohistochemistry-NBP1-76610-img0004.jpg)
![Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis in Jurkat cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP1-76610-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - Jurkat cells with caspase-8 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76610-img0002.jpg)
![Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - IF of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 ug/ml. Red: Caspase-8 Antibody (NBP1-76610). Blue: DAPI staining](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunofluorescence-NBP1-76610-img0007.jpg)
![Western Blot: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAF1B.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-OTI4F7-Western-Blot-NBP2-46274-img0005.jpg "Western Blot: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAF1B.")
![Immunohistochemistry: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CHAF1B-Antibody-OTI4F7-Immunohistochemistry-NBP2-46274-img0004.jpg)
![Western Blot: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/CHAF1B-Antibody-OTI4F7-Western-Blot-NBP2-46274-img0006.jpg)
![Immunohistochemistry: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CHAF1B-Antibody-OTI4F7-Immunohistochemistry-NBP2-46274-img0002.jpg)
![Immunohistochemistry: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CHAF1B-Antibody-OTI4F7-Immunohistochemistry-NBP2-46274-img0003.jpg)
![Immunofluorescence: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CHAF1B.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-OTI4F7-Immunofluorescence-NBP2-46274-img0001.jpg)
![Western Blot: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Necdin.](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Western-Blot-NBP2-46117-img0018.jpg "Western Blot: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Necdin.")
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0017.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0002.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0003.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0004.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0005.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0006.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0007.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0008.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0009.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0010.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0011.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0012.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0013.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0014.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0015.jpg)
![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0016.jpg)
![Immunofluorescence: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/Necdin-Antibody-OTI5D1-Immunofluorescence-NBP2-46117-img0001.jpg)
![Western Blot: Max Antibody [NB100-793] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Max-Antibody-Western-Blot-NB100-793-img0002.jpg "Western Blot: Max Antibody [NB100-793] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Max Antibody [NB100-793] - Staining of Prostate. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Max-Antibody-Immunohistochemistry-Paraffin-NB100-793-img0003.jpg)
![Immunofluorescence: Max Antibody [NB100-793] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Max-Antibody-Immunofluorescence-NB100-793-img0004.jpg)
![Immunofluorescence: Max Antibody [NB100-793] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Max-Antibody-Immunofluorescence-NB100-793-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NAIP Antibody [NBP1-77196] - Confocal microscopy analysis of NAIP expression in THP-1m with or without CFT073 stimulation. NLRC4 and NAIP expression in THP-1m, 6 h post stimulation with or without (mock) CFT073 was examined with confocal microscopy analysis using anti-NAIP antibody. Alexa fluor 488 (green) was used to detect anti-NAIP antibody. Nucleus of cells were stained with DAPI (blue). Results are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fmicb.2019.02020/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NAIP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77196-img0009.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NAIP Antibody [NBP1-77196] - Confocal microscopy analysis of NAIP expression in THP-1m with or without CFT073 stimulation. NLRC4 and NAIP expression in THP-1m, 6 h post stimulation with or without (mock) CFT073 was examined with confocal microscopy analysis using anti-NAIP antibody. Alexa fluor 488 (green) was used to detect anti-NAIP antibody. Nucleus of cells were stained with DAPI (blue). Results are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fmicb.2019.02020/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: NAIP Antibody [NBP1-77196] - Analysis of NLRP3, NLRC4, NAIP, AIM2, ASC, Caspase-4, Caspase-1, B-actin and GAPDH protein expression by immunoblotting. (A) Represents the immunoblotting results of NLRP3, NLRC4, NAIP, Caspase-1, GAPDH of UPEC infected UTI patients and controls group. Scattered plots showing individual densitometric values (IDV) of NAIP (D). Results were expressed as average densitometric ratio in patients and controls group +/- SD. P-value is ***p = 0.0001 and n.s, non-significant. anti-NLRP3 antibody (NBP1-77080), anti-NLRC4 antibody (NBP2-41124), anti-Caspase-1 antibody (NBP1-45433). Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fmicb.2019.02020/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NAIP-Antibody-Western-Blot-NBP1-77196-img0008.jpg)
![Western Blot: NAIP Antibody [NBP1-77196] - Analysis of NAIP in human tonsil tissue lysate with NAIP antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/NAIP-Antibody-Western-Blot-NBP1-77196-img0007.jpg)
![Immunocytochemistry: NAIP Antibody [NBP1-77196] - A549 cells with NAIP antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/NAIP-Antibody-Immunocytochemistry-NBP1-77196-img0002.jpg)
![Immunofluorescence: NAIP Antibody [NBP1-77196] - Immunofluorescence of NAIP in A549 cells with NAIP antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/NAIP-Antibody-Immunofluorescence-NBP1-77196-img0005.jpg)
![Immunohistochemistry: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TRIM45-Antibody-OTI3G3-Immunohistochemistry-NBP2-46223-img0006.jpg "Immunohistochemistry: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TRIM45-Antibody-OTI3G3-Immunohistochemistry-NBP2-46223-img0002.jpg)
![Immunohistochemistry: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TRIM45-Antibody-OTI3G3-Immunohistochemistry-NBP2-46223-img0003.jpg)
![Immunohistochemistry: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TRIM45-Antibody-OTI3G3-Immunohistochemistry-NBP2-46223-img0004.jpg)
![Immunohistochemistry: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TRIM45-Antibody-OTI3G3-Immunohistochemistry-NBP2-46223-img0005.jpg)
![Immunohistochemistry: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TRIM45-Antibody-OTI3G3-Immunohistochemistry-NBP2-46223-img0007.jpg)
![Immunofluorescence: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY TRIM45.](http://images.novusbio.com/fullsize/TRIM45-Antibody-OTI3G3-Immunofluorescence-NBP2-46223-img0001.jpg)
![Western Blot: BID Antibody (OTI1G10) [NBP2-46575] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BID.](http://images.novusbio.com/fullsize/BID-Antibody-OTI1G10-Western-Blot-NBP2-46575-img0006.jpg "Western Blot: BID Antibody (OTI1G10) [NBP2-46575] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BID.")
![Immunohistochemistry: BID Antibody (OTI1G10) [NBP2-46575] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BID-Antibody-OTI1G10-Immunohistochemistry-NBP2-46575-img0005.jpg)
![Western Blot: BID Antibody (OTI1G10) [NBP2-46575] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/BID-Antibody-OTI1G10-Western-Blot-NBP2-46575-img0007.jpg)
![Immunohistochemistry: BID Antibody (OTI1G10) [NBP2-46575] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BID-Antibody-OTI1G10-Immunohistochemistry-NBP2-46575-img0002.jpg)
![Immunohistochemistry: BID Antibody (OTI1G10) [NBP2-46575] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BID-Antibody-OTI1G10-Immunohistochemistry-NBP2-46575-img0003.jpg)
![Immunohistochemistry: BID Antibody (OTI1G10) [NBP2-46575] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BID-Antibody-OTI1G10-Immunohistochemistry-NBP2-46575-img0004.jpg)
![Immunofluorescence: BID Antibody (OTI1G10) [NBP2-46575] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/BID-Antibody-OTI1G10-Immunofluorescence-NBP2-46575-img0001.jpg)
![Western Blot: beta Tubulin Antibody (1B12) [NBP2-50061] - Western blot analysis of whole tissue or cell lysates using mouse mAb to Beta-tubulin, NBP2-50061, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat brain, [3] adult mouse brain, [4] NIH-3T3 cells, [5] HEK293 cells, [6] HeLa cells, [7] SH-SY5Y cells. The strong clean band with apparent SDS-PAGE molecular weight of ~50 kDa corresponds to the Beta-tubulin proteins.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-1B12-Western-Blot-NBP2-50061-img0004.jpg "Western Blot: beta Tubulin Antibody (1B12) [NBP2-50061] - Western blot analysis of whole tissue or cell lysates using mouse mAb to Beta-tubulin, NBP2-50061, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat brain, [3] adult mouse brain, [4] NIH-3T3 cells, [5] HEK293 cells, [6] HeLa cells, [7] SH-SY5Y cells. The strong clean band with apparent SDS-PAGE molecular weight of ~50 kDa corresponds to the Beta-tubulin proteins.")
![Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (1B12) [NBP2-50061] - HeLa cells stained with NBP2-50061 in green, and chicken antibody to Lamin A+C in red (NBP2-25152), and DNA in blue. NBP2-50061 reveals strong microtubular staining in the cytoplasm of HeLa cells, while the Lamin A+C localizes in the nuclear membrane.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-1B12-Immunocytochemistry-Immunofluorescence-NBP2-50061-img0003.jpg)
![Immunofluorescence: beta Tubulin Antibody (1B12) [NBP2-50061] - Immunofluorescent analysis of HeLa cells stained with NBP2-50061, dilution 1:5,000 in green, and costained with chicken pAb to the nucleic acid binding protein FOX2, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. NBP2-50061 produces strong staining of microtubules in the cytoplasm, while the FOX2 antibody labels the nuclei of HeLa cells.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-1B12-Immunofluorescence-NBP2-50061-img0005.jpg)