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![Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Western-Blot-NB100-615-img0010.jpg "Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.")
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Subcellular localization of mPARM-1 and hPARM-1 (full-length and mutant proteins). NIH/3T3 cells transiently expressing hPARM-1-GFP or deltaCT-GFP were fixed, immunostained for caveolin-1 (1:100, Novus Biologicals), and examined by confocal fluorescence microscopy. For hPARM-1-GFP-caveolin-1 co-localization, cells that demonstrated cell membrane PARM-1 localization were chosen. All co-localizations were observed following merging images of GFP-tagged proteins with those of Golgi, endosomes, plasma membrane, alpha-tubulin or caveolin-1 labeling. Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-84), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Immunocytochemistry-Immunofluorescence-NB100-615-img0014.jpg)
![Immunohistochemistry-Frozen: Caveolin-1 Antibody (7C8) [NB100-615] - Immunofluorescence of human adipose tissue. Primary antibody at 1:250. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Immunohistochemistry-Frozen-NB100-615-img0015.jpg)
![Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Flow-Cytometry-NB100-615-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Antibody at 1:200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane. Photo courtesy of Alberto Davalos, Yale School of Medicine.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Immunocytochemistry-Immunofluorescence-NB100-615-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Caveolin-1 [7C8] conjugated to Alexa Fluor 488 (NB100-615AF488) at 20 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Immunocytochemistry-Immunofluorescence-NB100-615-img0013.jpg)
![Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Caveolin 1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Flow-Cytometry-NB100-615-img0008.jpg)
![Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-7C8-Flow-Cytometry-NB100-615-img0011.jpg)
![Western Blot: Caveolin-1 Antibody [NBP2-54737] - Analysis in human cell line A-431 and human cell line CACO-2.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-Western-Blot-NBP2-54737-img0013.jpg "Western Blot: Caveolin-1 Antibody [NBP2-54737] - Analysis in human cell line A-431 and human cell line CACO-2.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Caveolin-1 Antibody [NBP2-54737] - Analysis in human lung and pancreas tissues. Corresponding CAV1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-54737-img0007.jpg)
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody [NBP2-54737] - Staining of human liver shows moderate membranous positivity in hepatic sinusoid cells.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-54737-img0014.jpg)
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody [NBP2-54737] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-54737-img0008.jpg)
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody [NBP2-54737] - Staining of human placenta shows strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-54737-img0010.jpg)
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody [NBP2-54737] - Staining of human lung shows strong membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-54737-img0011.jpg)
![Western Blot: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Western blot analysis of extracts of HeLa cells, using Caveolin-1antibody (NBP3-15615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-9I9J8-Western-Blot-NBP3-15615-img0017.jpg "Western Blot: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Western blot analysis of extracts of HeLa cells, using Caveolin-1antibody (NBP3-15615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Caveolin-1 Antibody (9I9J8) [NBP3-15615] -analysis of various lysates, using Caveolin-1 Rabbit mAb at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-western-blot-184202410142..jpg)
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Human colon using Caveolin-1 Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-immunohistochemistry-paraffin-184202410253..jpg)
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody (9I9J8) [NBP3-15615] -Human lung using Caveolin-1 Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-immunohistochemistry-paraffin-184202410355..jpg)
![Western Blot: Caveolin-1 Antibody (M281) [NBP3-23193] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23193_mouse-caveolin-1-mab-m281-119202316331613.jpg "Western Blot: Caveolin-1 Antibody (M281) [NBP3-23193] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (M281) [NBP3-23193] - Immunocytochemical labeling of caveolin-1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 antibodies, and detected using appropriate secondary antibodies conjugated to Cy3. Phase contrast images (left) and immunofluorescent images (right).](http://images.novusbio.com/fullsize/nbp3-23193_mouse-caveolin-1-mab-m281-19920231856940.jpg)
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody [NBP3-23245] - Immunocytochemical labeling of caveolin-1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 antibodies, and detected using appropriate secondary antibodies conjugated to Cy3. Phase contrast images (left) and immunofluorescent images (right).](http://images.novusbio.com/fullsize/nbp3-23245_rabbit-caveolin-1-pab-199202317594410.jpg "Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody [NBP3-23245] - Immunocytochemical labeling of caveolin-1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 antibodies, and detected using appropriate secondary antibodies conjugated to Cy3. Phase contrast images (left) and immunofluorescent images (right).")
![Western Blot: Caveolin-1 Antibody [NBP3-23245] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23245_rabbit-caveolin-1-pab-19920231410121.jpg)
![Western Blot: Caveolin-1 [p Tyr14] Antibody (M283) [NBP3-23246] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23246_mouse-caveolin-1-p-tyr14-mab-m283-19920231856942.jpg "Western Blot: Caveolin-1 [p Tyr14] Antibody (M283) [NBP3-23246] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: Caveolin-1 [p Tyr14] Antibody (M283) [NBP3-23246] - Immunocytochemical labeling of caveolin-1 phosphorylation in rabbit spleen fibroblasts. The cells were treated with pervanadate (1 mM) for 30 min, then fixed with paraformaldehyde and labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 (Tyr-14) antibodies. The antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23246_mouse-caveolin-1-p-tyr14-mab-m283-199202312523624.jpg)
![Western Blot: Caveolin-1 Antibody (RM325) [NBP2-77410] - HeLa cell lysate using anti-Caveolin-1 rabbit monoclonal antibody (Clone RM325) at a 1:40,000 dilution.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-RM325-Western-Blot-NBP2-77410-img0002.jpg "Western Blot: Caveolin-1 Antibody (RM325) [NBP2-77410] - HeLa cell lysate using anti-Caveolin-1 rabbit monoclonal antibody (Clone RM325) at a 1:40,000 dilution.")
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody (RM325) [NBP2-77410] - Staining of human liver tissue section at a 1:1000 dilution.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-RM325-Immunohistochemistry-NBP2-77410-img0001.jpg)
![Western Blot: Caveolin-1 Antibody (S01-9C9) [NBP3-20077] - Western blot detection of Caveolin-1 in Hela cell lysates using NBP3-20077 (1:1000 diluted). Predicted band size: 20kDa. Observed band size: 20kDa.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-S01-9C9-Western-Blot-NBP3-20077-img0002.jpg "Western Blot: Caveolin-1 Antibody (S01-9C9) [NBP3-20077] - Western blot detection of Caveolin-1 in Hela cell lysates using NBP3-20077 (1:1000 diluted). Predicted band size: 20kDa. Observed band size: 20kDa.")
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody (S01-9C9) [NBP3-20077] - Immunohistochemistry of Caveolin-1 in paraffin-embedded Human breast cancer tissue using NBP3-20077 at dilution 1/20](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-S01-9C9-Immunohistochemistry-Paraffin-NBP3-20077-img0001.jpg)
![Western Blot: Caveolin-1 Antibody (2A7) [NBP3-26695] - Positive Western Blot detected in: THP-1 whole cell lysate. All lanes: Caveolin-1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 20 kDa Observed band size: 20 kDa](http://images.novusbio.com/fullsize/nbp3-26695_rabbit-caveolin-1-mab-2a7-28220241140941.jpg "Western Blot: Caveolin-1 Antibody (2A7) [NBP3-26695] - Positive Western Blot detected in: THP-1 whole cell lysate. All lanes: Caveolin-1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 20 kDa Observed band size: 20 kDa")
![Flow Cytometry: Caveolin-1 Antibody (2A7) [NBP3-26695] - Overlay Peak curve showing Hela cells stained with Caveolin-1 Antibody (2A7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26695_rabbit-caveolin-1-mab-2a7-282202414312730.jpg)
![ELISA: Mouse Caveolin-1 ELISA Kit (Colorimetric) [NBP2-75049] - Samples were spiked with high concentrations of Mouse Caveolin-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75049_mouse-caveolin-1-elisa-kit-colorimetric-13220241681491.jpg "ELISA: Mouse Caveolin-1 ELISA Kit (Colorimetric) [NBP2-75049] - Samples were spiked with high concentrations of Mouse Caveolin-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Caveolin-1 ELISA Kit (Colorimetric) [NBP2-75049] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75049_mouse-caveolin-1-elisa-kit-colorimetric-432024856739.jpg)
![ELISA: Rat Caveolin-1 ELISA Kit (Colorimetric) [NBP2-75052] - Samples were spiked with high concentrations of Rat Caveolin-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75052_rat-caveolin-1-elisa-kit-colorimetric-132202416173457.jpg "ELISA: Rat Caveolin-1 ELISA Kit (Colorimetric) [NBP2-75052] - Samples were spiked with high concentrations of Rat Caveolin-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Caveolin-1 ELISA Kit (Colorimetric) [NBP2-75052] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75052_rat-caveolin-1-elisa-kit-colorimetric-4320249141660.jpg)
![ELISA: Monkey Caveolin-1 ELISA Kit (Colorimetric) [NBP2-75051] - Samples were spiked with high concentrations of Monkey Caveolin-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75051_monkey-caveolin-1-elisa-kit-colorimetric-1322024168537.jpg "ELISA: Monkey Caveolin-1 ELISA Kit (Colorimetric) [NBP2-75051] - Samples were spiked with high concentrations of Monkey Caveolin-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Monkey Caveolin-1 ELISA Kit (Colorimetric) [NBP2-75051] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75051_monkey-caveolin-1-elisa-kit-colorimetric-432024955063.jpg)