![Western Blot: Wilms Tumor 1 Overexpression Lysate (Adult Normal) [NBP2-11141] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Wilms Tumor 1.](http://images.novusbio.com/fullsize/Wilms-Tumor-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11141-img0001.jpg "Western Blot: Wilms Tumor 1 Overexpression Lysate (Adult Normal) [NBP2-11141] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Wilms Tumor 1.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | WT1 Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC220079 Accession#: NM_000378 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | WT1 |
| Dilutions |
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| Application Notes | This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
| Theoretical MW | 54.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
Research Areas for WT1 Lysate (NBP2-11141)Find related products by research area.
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Routine WT1 Antibody Screen Uncovers an Exciting New Cancer-Cleaning Enzyme Enzyme antibodies are widely used in both apoptosis and cancer studies, as disruption of the proteins regulating apoptosis is known to lead to formation of tumor cells in certain cancers. We at Novus Biologicals are continually growing our enzyme anti... Read full blog post. |
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| Gene Symbol | WT1 |
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![Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Whole cell protein from human K562, HEK293 and kidney tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WT1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](https://images.novusbio.com/images/WT1-Antibody-6F-H2-Western-Blot-NB110-60011-img0005.jpg "Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Whole cell protein from human K562, HEK293 and kidney tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WT1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")