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Wnt-1 Antibody (13F9) [Alexa Fluor® 750]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA
Clone
13F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750

Order Details

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Wnt-1 Antibody (13F9) [Alexa Fluor® 750] Summary

Immunogen
Synthetic peptide corresponding to an internal region of human Wnt-1 protein. (Uniprot: P04628)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
WNT1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with Wnt-1 from mouse, human, rat, bovine, dog, macaque, opossum and rat based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt-1 based on a 91% sequence homology. Cross-reactivity with Wnt-1 from other sources has not been determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Wnt-1 Antibody (13F9) [Alexa Fluor® 750]

  • Int-1
  • INT1Proto-oncogene Int-1 homolog
  • proto-oncogene Wnt-1
  • wingless-type MMTV integration site family, member 1 (oncogene INT1)
  • wingless-type MMTV integration site family, member 1
  • Wnt1
  • Wnt-1

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol WNT1