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WIPI1 Antibody [mFluor Violet 450 SE]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
mFluor Violet 450 SE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-82048AF350
Alexa Fluor 405
NBP2-82048AF405
Alexa Fluor 488
NBP2-82048AF488
Alexa Fluor 532
NBP2-82048AF532
Alexa Fluor 594
NBP2-82048AF594
Alexa Fluor 647
NBP2-82048AF647
Alexa Fluor 700
NBP2-82048AF700
Alexa Fluor 750
NBP2-82048AF750
Biotin
NBP2-82048B
CoraFluor 1
NBP2-82048CL1
DyLight 350
NBP2-82048UV
DyLight 405
NBP2-82048V
DyLight 488
NBP2-82048G
DyLight 550
NBP2-82048R
DyLight 594
NBP2-82048DL594
DyLight 650
NBP2-82048C
DyLight 680
NBP2-82048FR
DyLight 755
NBP2-82048IR
FITC
NBP2-82048F
HRP
NBP2-82048H
Janelia Fluor 525
NBP2-82048JF525
Janelia Fluor 549
NBP2-82048JF549
Janelia Fluor 646
NBP2-82048JF646
Janelia Fluor 669
NBP2-82048JF669
PerCP
NBP2-82048PCP
Unconjugated
NBP2-82048
mFluor Violet 500 SE
NBP2-82048MFV500
mFluor Violet 610 SE
NBP2-82048MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-82048
BSA Free
NBP2-82048
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WIPI1 Antibody [mFluor Violet 450 SE] Summary

Immunogen
WIPI1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human WIPI1. The immunogen is located within amino acids 350 - 400 of WIPI1. Amino Acid Squence: LGSGTTEENKENDLRPS
Specificity
At least two isoforms of WIPI1 are known to exist; this antibody will detect both isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WIPI1
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for WIPI1 Antibody [mFluor Violet 450 SE]

  • Atg18 protein homolog
  • Atg18
  • ATG18A
  • FLJ10055
  • WD repeat domain phosphoinositide-interacting protein 1
  • WD repeat domain, phosphoinositide interacting 1
  • WD40 repeat protein interacting with phosphoinositides of 49 kDa
  • WD40 repeat protein Interacting with phosphoInositides of 49kDa
  • WIPI 49 kDa
  • WIPI-1 alpha
  • WIPI-1
  • WIPI49ATG18

Background

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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WD repeat domain phosphoinositide-interacting protein 1 (WIPI) is involved in the lysosomal degradation of cytoplasmic components during starvation-induced autophagy. WIPI1 is a seven bladed beta-propeller protein that provides a scaffold for the ...  Read full blog post.
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Bioinformatics

Gene Symbol WIPI1