Western Blot: WDR79 Antibody [NBP1-92592] - Analysis in control (vector only transfected HEK293T lysate) and WRAP53 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: WDR79 Antibody [NBP1-92592] - Staining of human cell line A-431 shows localization to nuclear bodies.
Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592] - Staining of human skin shows weak nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.
Independent Antibodies: Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592] - Staining of human fallopian tube, kidney, liver and skin using Anti-WRAP53 antibody NBP1-92592 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592] - Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592] - Staining of human liver shows no positivity in hepatocytes as expected.
This antibody was developed against Recombinant Protein corresponding to amino acids: GSLSEEEANGPELGSGKAMEDTSGEPAAEDEGDTAWNYSFSQLPRFLSGSWSEFSTQPENFLKGCKWAPDGSCILTNSADNILRIYNLPPELYHEGEQVEYAEMVPVLRMVEGDTIYDYC
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WRAP53
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.Use in Western blot reported in scientific literature (PMID 28347242).
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (81%). Human reactivity reported in scientific literature (PMID: 26426684).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for WDR79 Antibody
FLJ10385
TCAB1WDR79telomerase Cajal body protein 1
WD repeat containing, antisense to TP53
WD repeat domain 79
WD repeat-containing protein 79
WD40 protein Wrap53
WD40 repeat-containing protein encoding RNA antisense to p53
WD-encoding RNA antisense to p53
Background
WD-repeat domain 79 (WDR79) contains six WD (tryptophan-aspartate) repeat domains found in a number of proteins that function as adaptor molecules in signal transduction and cytoskeletal organization. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. The function of the WDR79 protein has not been characterized, however significant and consistent single nucleotide polymorphisms in the WDR79 gene have been found to be associated with ER negative breast cancer.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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