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VPS29 Antibody

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Western Blot: VPS29 Antibody [NB100-1387] - (0.1ug/ml) of Human Spleen lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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VPS29 Antibody Summary

Immunogen
Peptide with sequence C-DDVKVERIEYKKP corresponding to C-Terminus according to NP_057310.1, NP_476528.1.
Specificity
This antibody is expected to recognise both human isoforms (represented by NP_057310.1; NP_476528.1).
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VPS29
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 20+21 kDa band bands observed in human spleen lysates (calculated MW of 20.5 kDa band according to NP_057310.1 and 20.9 kDa band according to NP_476528.1).
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Spleen Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-1387.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VPS29 Antibody

  • DC15
  • DC7
  • DKFZp564F0223
  • EC 3.1.3.3
  • FLJ20492
  • hVPS29
  • PEP11 homolog
  • PEP11
  • retromer protein
  • vacuolar protein sorting 29 (yeast homolog)
  • vacuolar protein sorting 29 (yeast)
  • vacuolar protein sorting 29 homolog (S. cerevisiae)
  • vacuolar protein sorting-associated protein 29
  • vacuolar sorting protein VPS29/PEP11
  • Vesicle protein sorting 29
  • x 007 protein

Background

VPS29 is an essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS29 Antibody (NB100-1387)(1)

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol VPS29
Entrez
Uniprot