| Reactivity | MuSpecies Glossary |
| Applications | WB, Flow, CyTOF-ready, ICC/IF |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | FAB7005G-100UG | |||
| Allophycocyanin | FAB7005A | |||
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse VISTA/B7-H5/PD-1H Phe33-Ala191 Accession # Q9D659 |
| Specificity | Detects mouse VISTA/B7-H5/PD-1H in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human VISTA/B7-H5/PD-1H is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Sheep |
| Gene | Vsir |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.2 mg/mL. |
Platelet Receptor Gi24 (also known as VISTA, B7-H5, SISP1, C10orf54 and , Dies1 [Differentiation of ESC-1]) is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, Gi24 appears to positively interact with BMP4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity, and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature mouse Gi24 is a 277 amino acid (aa) type I transmembrane glycoprotein (aa 33-309). It contains a 149 aa extracellular region (aa 33-191) with one V-type Ig-like domain (aa 33-161) and a 97 aa cytoplasmic domain. Based on human Gi24, mouse Gi24 will likely undergo proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment, plus a 25-30 kDa membrane-bound fragment. There are two potential isoform variants. One contains a deletion of aa 127-187, while another shows an alternative start site at Met82. Over aa 33-191, mouse Gi24/Dies1 shares 78% and 70% aa identity with rat and human Gi24, respectively.
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| Publication using AF7005 | Applications | Species |
|---|---|---|
| Fu, J;Li, S;Ma, H;Yang, J;Pagnotti, GM;Brown, LM;Weiss, SJ;Mapara, MY;Lentzsch, S; The checkpoint inhibitor PD-1H/VISTA controls osteoclast-mediated multiple myeloma bone disease Nature communications 2023-07-17 [PMID: 37460553] (Immunoprecipitation, Human) | Immunoprecipitation | Human |
Secondary Antibodies |
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