Home » VE-Statin/EGFL7 » VE-Statin/EGFL7 Lysates » VE-Statin/EGFL7 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

VE-Statin/EGFL7 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: EGFL7 Overexpression Lysate (Adult Normal) [NBL1-10150] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EGFL7.
Western Blot: EGFL7 Overexpression Lysate (Adult Normal) [NBL1-10150] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EGFL7.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals offers 100% Guarantee

VE-Statin/EGFL7 Overexpression Lysate Summary

Description

VE-Statin/EGFL7 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC218849

Accession#: NM_016215

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
EGFL7

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for VE-Statin/EGFL7 Overexpression Lysate

  • EGFL7
  • EGF-like protein 7
  • EGF-like-domain, multiple 7
  • epidermal growth factor-like protein 7
  • MEGF7
  • MGC111117
  • Multiple EGF-like domains protein 7
  • Multiple epidermal growth factor-like domains protein 7
  • NEU1 protein
  • NOTCH4-like protein
  • RP11-251M1.2
  • Vascular endothelial statin
  • VEStatin
  • VE-Statin
  • ZNEU1

Background

Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate in vitro

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Cathepsin
A/Lysosomal Carboxype ...
AF1049
Immunohistochemistry Cathepsin A/Lysosomal Carboxypeptidase A Antibody
(1 Review)
(6 Publications)
Species: Hu
Applications: IHC, WB
NEU3 Antibody -
Azide and BSA Free
NBP3-04868
Western Blot NEU3 Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence NEU3 Antibody - Azide and BSA Free
Species: Hu, Mu
Applications: ELISA, ICC/IF, WB

beta-Galactosidase-1/G ...
NBP2-45731
Western Blot beta-Galactosidase-1/GLB1 Antibody (OTI1C9)
Immunohistochemistry beta-Galactosidase-1/GLB1 Antibody (OTI1C9)
Immunohistochemistry beta-Galactosidase-1/GLB1 Antibody (OTI1C9)
(6 Publications)
Species: Ca, Hu, Pm
Applications: IHC, IHC-P, WB
NEU2 Antibody
(OTI4F4)
NBP2-00783
Flow Cytometry NEU2 Antibody (OTI4F4)
Western Blot NEU2 Antibody (OTI4F4)
Immunocytochemistry/ Immunofluorescence NEU2 Antibody (OTI4F4)
(1 Publication)
Species: Hu
Applications: Flow, ICC/IF, WB
NEU4 Antibody -
BSA Free
NBP2-32682
Immunohistochemistry NEU4 Antibody - BSA Free
Species: Hu
Applications: IHC, IHC-P
Elastin Antibody
(BA-4)
NB100-2076
Immunohistochemistry-Paraffin Elastin Antibody (BA-4)
Immunohistochemistry-Paraffin Elastin Antibody (BA-4)
Immunohistochemistry-Paraffin Elastin Antibody (BA-4)
(2 Reviews)
(11 Publications)
Species: Bv, Fe, Hu, Rb
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(686 Publications)
Species: Hu
Applications: ELISA
MMP-9 [HRP]
DMP900
N/A MMP-9 [HRP]
N/A MMP-9 [HRP]
(220 Publications)
Species: Hu
Applications: ELISA
TLR4 Antibody
(76B357.1) - BSA Free
NB100-56566
Dual RNAscope ISH-IHC TLR4 Antibody (76B357.1) - BSA Free
Immunohistochemistry TLR4 Antibody (76B357.1) - BSA Free
Immunohistochemistry TLR4 Antibody (76B357.1) - BSA Free
(5 Reviews)
(145 Publications)
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, Simple Western, WB
ICAM-1/CD54
Antibody [Unconjugate
AF796
Immunohistochemistry ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
(6 Reviews)
(89 Publications)
Species: Mu
Applications: AdBlk, IHC, WB
EGF [Unconjugated]
236-EG
SDS-PAGE EGF [Unconjugated]
Bioactivity EGF [Unconjugated]
Cell Culture EGF [Unconjugated]
(865 Publications)
Species: Hu
Applications: BA
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
EGFL8 Antibody -
BSA Free
NBP2-49327
Immunocytochemistry/ Immunofluorescence EGFL8 Antibody - BSA Free
Immunohistochemistry-Paraffin EGFL8 Antibody - BSA Free
Immunohistochemistry-Paraffin EGFL8 Antibody - BSA Free
Species: Hu
Applications: ICC/IF, IHC, IHC-P
LDLR Antibody
[Unconjugated]
AF2255
Immunohistochemistry LDLR Antibody [Unconjugated]
Western Blot LDLR Antibody [Unconjugated]
Western Blot LDLR Antibody [Unconjugated]
(47 Publications)
Species: Mu
Applications: Block, CyTOF-ready, Flow, IHC, WB
Glutaminyl-peptide
Cyclotransferase/QPCT ...
NBP1-81838
Western Blot Glutaminyl-peptide Cyclotransferase/QPCT Antibody - BSA Free
Immunohistochemistry Glutaminyl-peptide Cyclotransferase/QPCT Antibody - BSA Free
Immunohistochemistry-Paraffin Glutaminyl-peptide Cyclotransferase/QPCT Antibody - BSA Free
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
VE-Statin/EGFL7
Overexpression Lysate
NBL1-10150
VE-Statin/EGFL7 Overexpression Lysate
Species: Hu
Applications: WB

Publications for VE-Statin/EGFL7 Lysate (NBL1-10150) (0)

There are no publications for VE-Statin/EGFL7 Lysate (NBL1-10150).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for VE-Statin/EGFL7 Lysate (NBL1-10150) (0)

There are no reviews for VE-Statin/EGFL7 Lysate (NBL1-10150). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VE-Statin/EGFL7 Lysate (NBL1-10150) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional VE-Statin/EGFL7 Products

Research Areas for VE-Statin/EGFL7 Lysate (NBL1-10150)

Find related products by research area.

Blogs on VE-Statin/EGFL7

There are no specific blogs for VE-Statin/EGFL7, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our VE-Statin/EGFL7 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol EGFL7