![Western Blot: VBP1 Antibody (2A3) [NBP2-03754] Analysis of extracts (35ug) from 9 different cell lines by using anti-VBP1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/VBP1-Antibody-2A3-Western-Blot-NBP2-03754-img0008.jpg "Western Blot: VBP1 Antibody (2A3) [NBP2-03754] Analysis of extracts (35ug) from 9 different cell lines by using anti-VBP1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | OTI2A3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human VBP1(NP_003363) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | VBP1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 22.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: VBP1 Antibody (2A3) [NBP2-03754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VBP1.](http://images.novusbio.com/fullsize/VBP1-Antibody-2A3-Immunocytochemistry-Immunofluorescence-NBP2-03754-img0007.jpg "Immunocytochemistry/Immunofluorescence: VBP1 Antibody (2A3) [NBP2-03754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VBP1.")
![Immunohistochemistry-Paraffin: VBP1 Antibody (2A3) [NBP2-03754] - Staining of paraffin-embedded Human tonsil using anti-VBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VBP1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP2-03754-img0006.jpg "Immunohistochemistry-Paraffin: VBP1 Antibody (2A3) [NBP2-03754] - Staining of paraffin-embedded Human tonsil using anti-VBP1 mouse monoclonal antibody.")
![Western Blot: VBP1 Antibody (2A3) [NBP2-03754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VBP1.](http://images.novusbio.com/fullsize/VBP1-Antibody-2A3-Western-Blot-NBP2-03754-img0001.jpg "Western Blot: VBP1 Antibody (2A3) [NBP2-03754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VBP1.")
![Immunohistochemistry-Paraffin: VBP1 Antibody (2A3) [NBP2-03754] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VBP1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP2-03754-img0002.jpg "Immunohistochemistry-Paraffin: VBP1 Antibody (2A3) [NBP2-03754] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VBP1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: VBP1 Antibody (2A3) [NBP2-03754] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-VBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VBP1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP2-03754-img0003.jpg "Immunohistochemistry-Paraffin: VBP1 Antibody (2A3) [NBP2-03754] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-VBP1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: VBP1 Antibody (2A3) [NBP2-03754] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-VBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VBP1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP2-03754-img0004.jpg "Immunohistochemistry-Paraffin: VBP1 Antibody (2A3) [NBP2-03754] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-VBP1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: VBP1 Antibody (2A3) [NBP2-03754] - Staining of paraffin-embedded Human Kidney tissue using anti-VBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VBP1-Antibody-2A3-Immunohistochemistry-Paraffin-NBP2-03754-img0005.jpg "Immunohistochemistry-Paraffin: VBP1 Antibody (2A3) [NBP2-03754] - Staining of paraffin-embedded Human Kidney tissue using anti-VBP1 mouse monoclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")