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CD177 Antibody

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Human peripheral blood lymphocytes were stained with Goat Anti-Human CD177 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3505, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Human peripheral blood lymphocytes were stained with Goat Anti-Human CD177 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3505, filled histogram) or isotype control antibody (Catalog # AB-108-C, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3505-SP
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CD177 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD177
Leu22-Gly407
Accession # AAH29167
Specificity
Detects human CD177 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD177
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF3505 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD177 Antibody

  • CD177 antigenNB1 GP
  • CD177 molecule
  • CD177
  • HNA-2a
  • HNA2Acell surface receptor
  • Human neutrophil alloantigen 2a
  • NB1 GP
  • NB1PRV-1
  • polycythemia rubra vera 1
  • Polycythemia rubra vera protein 1
  • PRV1
  • PRV1NB1 glycoprotein

Background

CD177 is a polymorphic gene in the leukocyte antigen (Ly6) uPAR superfamily. It encodes at least two alleles of the neutrophilic membrane protein PRV1 (Polycythemic Rubra Vera protein 1) and NB1 (also named HNA-2a) that are glycosyl-phosphotidylinositol (GP1) anchored proteins of 58‑64 kDa. CD177 has two highly homologous cysteine-rich uPAR/Ly6 domains. The mRNA levels of CD177 is elevated under conditions where neutrophil counts are increased and is a useful marker for polycythemic vera. Mature human and mouse CD177 share 43% amino acid sequence identity. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (1-3).
  1. Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
  2. Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
  3. Temerinac, S. et al. (2000) Blood 95:2569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD177 Antibody (AF3505)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, Western Blot.


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Bioinformatics

Gene Symbol CD177
Uniprot