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Recombinant E. coli UNG His Protein

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SDS-Page: uracil-DNA glycosylase Protein [NBP2-22637]
SDS-Page: uracil-DNA glycosylase Protein [NBP2-22637]

Product Details

Summary
Reactivity EcSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant E. coli UNG His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-229 of E.coli UNG

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA FRFTELGDVK VVILGQDPYH GPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHRE GVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN QWLEQRGETP IDWMPVLPAE SE
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
UNG
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
28.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant E. coli UNG His Protein

  • DGU
  • DGUEC 3.2.2
  • HIGM4EC 3.2.2.27
  • UDG
  • UDGDKFZp781L1143
  • UNG
  • UNG15
  • UNG1uracil-DNA glycosylase 1, uracil-DNA glycosylase 2
  • UNG2
  • uracil-DNA glycosylase

Background

Ung, also known as Uracil-DNA glycosylase, is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. After a mutation occurs, the mutagenic threat of uracil propagates through any subsequent DNA replication steps. Recombinant E.coli ung protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol UNG