![Immunocytochemistry/Immunofluorescence: U1A Antibody [NBP2-53095] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SNRPA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.](http://images.novusbio.com/fullsize/U1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53095-img0001.jpg "Immunocytochemistry/Immunofluorescence: U1A Antibody [NBP2-53095] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SNRPA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Rb, RM, Sh, Xp, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human SNRPA. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Porcine (100%), Bovine (100%), Rabbit (98%), Rhesus Macaque (100%), Zebrafish (90%), Xenopus (91%), Sheep (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SNRPA |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 31 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.0), 1.0% BSA, and 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Immunogen affinity purified |
| Publication using NBP2-53095 | Applications | Species |
|---|---|---|
| Kour S, Rajan DS, Fortuna TR et al. Loss of function mutations in GEMIN5 cause a neurodevelopmental disorder Nature communications 2021-05-07 [PMID: 33963192] (WB, ICC/IF, Human) | WB, ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for U1A Antibody (NBP2-53095)Find related products by research area.
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| Gene Symbol | SNRPA |
![Immunohistochemistry-Paraffin: U1A Antibody [NBP2-53095] - SNRPA antibody detects SNRPA protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SNRPA antibody dilution: 1:500.](http://images.novusbio.com/fullsize/U1A-Antibody-Immunohistochemistry-Paraffin-NBP2-53095-img0008.jpg "Immunohistochemistry-Paraffin: U1A Antibody [NBP2-53095] - SNRPA antibody detects SNRPA protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SNRPA antibody dilution: 1:500.")
![Immunohistochemistry-Paraffin: U1A Antibody [NBP2-53095] - Analysis of paraffin-embedded human breast cancer, using SNRPA antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/U1A-Antibody-Immunohistochemistry-Paraffin-NBP2-53095-img0002.jpg "Immunohistochemistry-Paraffin: U1A Antibody [NBP2-53095] - Analysis of paraffin-embedded human breast cancer, using SNRPA antibody at 1:250 dilution.")
![Immunohistochemistry-Paraffin: U1A Antibody [NBP2-53095] - SNRPA antibody detects SNRPA protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. SNRPA antibody dilution: 1:500.](http://images.novusbio.com/fullsize/U1A-Antibody-Immunohistochemistry-Paraffin-NBP2-53095-img0003.jpg "Immunohistochemistry-Paraffin: U1A Antibody [NBP2-53095] - SNRPA antibody detects SNRPA protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. SNRPA antibody dilution: 1:500.")
![Immunohistochemistry-Paraffin: U1A Antibody [NBP2-53095] - SNRPA antibody detects SNRPA protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SNRPA antibody dilution: 1:500.](http://images.novusbio.com/fullsize/U1A-Antibody-Immunohistochemistry-Paraffin-NBP2-53095-img0006.jpg "Immunohistochemistry-Paraffin: U1A Antibody [NBP2-53095] - SNRPA antibody detects SNRPA protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SNRPA antibody dilution: 1:500.")
![Immunohistochemistry-Paraffin: U1A Antibody [NBP2-53095] - SNRPA antibody detects SNRPA protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. SNRPA antibody dilution: 1:500.](http://images.novusbio.com/fullsize/U1A-Antibody-Immunohistochemistry-Paraffin-NBP2-53095-img0007.jpg "Immunohistochemistry-Paraffin: U1A Antibody [NBP2-53095] - SNRPA antibody detects SNRPA protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. SNRPA antibody dilution: 1:500.")
![Western Blot: U1A Antibody [NBP2-53095] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNRPA antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-53095_rabbit-polyclonal-u1a-antibody-western-blot-105202485919..jpg "Western Blot: U1A Antibody [NBP2-53095] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNRPA antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
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![Immunohistochemistry Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_Immunohistochemistry_10732.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")