![Western Blot: TUFM Antibody (CL2243) [NBP2-36752] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/TUFM-Antibody-CL2243-Western-Blot-NBP2-36752-img0005.jpg "Western Blot: TUFM Antibody (CL2243) [NBP2-36752] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | CL2243 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids:AVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSI |
| Epitope | RDLEKPFLLPVEAVY |
| Predicted Species | Mouse (95%), Rat (96%). Backed by our 100% Guarantee. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TUFM |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining in U251 cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TUFM-Antibody-CL2243-Immunocytochemistry-Immunofluorescence-NBP2-36752-img0011.jpg "Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining in U251 cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts, as well as in Leydig cells.](http://images.novusbio.com/fullsize/TUFM-Antibody-CL2243-Immunohistochemistry-Paraffin-NBP2-36752-img0015.jpg "Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts, as well as in Leydig cells.")
![Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining in HeLa cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TUFM-Antibody-CL2243-Immunocytochemistry-Immunofluorescence-NBP2-36752-img0007.jpg "Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining in HeLa cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.")
![Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human cell line A431 showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TUFM-Antibody-CL2243-Immunocytochemistry-Immunofluorescence-NBP2-36752-img0008.jpg "Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human cell line A431 showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.")
![Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining in MCF7 cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TUFM-Antibody-CL2243-Immunocytochemistry-Immunofluorescence-NBP2-36752-img0009.jpg "Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining in MCF7 cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.")
![Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining in U2OS cell line with Anti-TUFM monoclonal antibody, showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TUFM-Antibody-CL2243-Immunocytochemistry-Immunofluorescence-NBP2-36752-img0010.jpg "Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining in U2OS cell line with Anti-TUFM monoclonal antibody, showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human fallopian tube shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TUFM-Antibody-CL2243-Immunohistochemistry-Paraffin-NBP2-36752-img0012.jpg "Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human fallopian tube shows strong granular cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TUFM-Antibody-CL2243-Immunohistochemistry-Paraffin-NBP2-36752-img0013.jpg "Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules and moderate positivity in glomeruli.](http://images.novusbio.com/fullsize/TUFM-Antibody-CL2243-Immunohistochemistry-Paraffin-NBP2-36752-img0014.jpg "Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules and moderate positivity in glomeruli.")
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