![Western Blot: TUFM Antibody (1A2) [H00007284-M03] - Analysis of TUFM expression in transfected 293T cell line by TUFM monoclonal antibody (M03), clone 1A2. Lane 1: TUFM transfected lysatE (49.9 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TUFM-Antibody-1A2-Western-Blot-H00007284-M03-img0004.jpg "Western Blot: TUFM Antibody (1A2) [H00007284-M03] - Analysis of TUFM expression in transfected 293T cell line by TUFM monoclonal antibody (M03), clone 1A2. Lane 1: TUFM transfected lysatE (49.9 KDa). Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IP |
| Clone | 1A2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | TUFM (AAH01633, 40 a.a. ~ 455 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAATTKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG |
| Specificity | TUFM - Tu translation elongation factor, mitochondrial (1A2) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TUFM |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Immunoprecipitation: TUFM Antibody (1A2) [H00007284-M03] - Analysis of TUFM transfected lysate using anti-TUFM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TUFM rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/TUFM-Antibody-1A2-Immunoprecipitation-H00007284-M03-img0002.jpg "Immunoprecipitation: TUFM Antibody (1A2) [H00007284-M03] - Analysis of TUFM transfected lysate using anti-TUFM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TUFM rabbit polyclonal antibody.")
![Sandwich ELISA: TUFM Antibody (1A2) [H00007284-M03] - Detection limit for recombinant GST tagged TUFM is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TUFM-Antibody-1A2-Sandwich-ELISA-H00007284-M03-img0003.jpg "Sandwich ELISA: TUFM Antibody (1A2) [H00007284-M03] - Detection limit for recombinant GST tagged TUFM is 0.3 ng/ml as a capture antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")