Tsukushi/TSK Antibody

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

TSK, also known as Tsukushi, is an atypical member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins (1, 2). All contain leucine-rich repeats (LRR) flanked by conserved cysteines, but the C-terminus of TSK resembles the structure of nyctalopin and chondroadherin rather than class I‑III SLRPs (1). The human TSK cDNA encodes a 16 amino acid (aa) signal sequence and a 337 aa secreted (presumably glycosylated) protein that contains nine LRR (2). Proteoglycan modification of TSK has not been shown. SLRP-like activity or expression of TSK has been identified in several contexts. TSK mRNA is upregulated by estrogen treatment in breast cancer cell lines, and so is proposed to be involved in hormonally regulated extracellular matrix remodeling (2). TSK is upregulated along with bone markers in Vitamin K2-treated osteosarcoma cell lines, mediated by the pregnane X receptor, PXR (3). TSK was also shown to contribute to vitamin K2-mediated enhancement of collagen accumulation. Other SLRPs have been shown to form a protective coat around collagen fibrils, shielding them from proteolysis (4). In rats, expression of TSK in liver, intestines and islets of Langerhans resembles the pattern of Glut2 (5). TSK transcription is upregulated by both estrogen and insulin in hepatocytes. In Xenopus and chick embryos, TSK is expressed in the middle primitive streak, where it binds and antagonizes BMP-4 and promotes dorsalization and formation of the neural crest and the primitive organizer (6, 7). Xenopus TSK also binds the extracellular region of delta-1, modulating Notch activity (7). Human TSK shows 87%, 86%, 86%, 85%, 52% and 50% aa identity with canine, mouse, rat, bovine, chick, and Xenopus TSK, respectively, with the highest identity in the first four LRR.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Array AF3940

• Tsukushi/TSK Antibodies
• Tsukushi/TSK Lysate
• Tsukushi/TSK Protein
• Tsukushi/TSK Proteins and Enzyme