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Human Troponin T type 1 (slow skeletal) ELISA Kit (Colorimetric)

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ELISA: Human Troponin T type 1 (slow skeletal) ELISA Kit (Colorimetric) [NBP2-76601] - Samples were spiked with high concentrations of Human Troponin T type 1 (slow skeletal) and diluted with Reference Standard & Sample ...read more
ELISA: Human Troponin T type 1 (slow skeletal) ELISA Kit (Colorimetric) [NBP2-76601] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.625 - 1000pg/mL
Sensitivity
9.375pg/mL

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Human Troponin T type 1 (slow skeletal) ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human TNNT1 in samples. No significant cross-reactivity or interference between Human TNNT1 and analogues was observed.
Standard Curve Range
15.625 - 1000pg/mL
Sensitivity
9.375pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.88
Intra-Assay
CV% < 4.97
Spike Recovery
89-109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNNT1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Troponin T type 1 (slow skeletal) ELISA Kit (Colorimetric)

  • ANMtroponin T, slow skeletal muscle
  • FLJ98147
  • MGC104241
  • Slow skeletal muscle troponin T
  • STNT
  • TnTs
  • TNTtroponin T1, skeletal, slow
  • troponin T type 1 (skeletal, slow)
  • troponin-T1, skeletal, slow

Background

Troponin T type 1 encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol TNNT1