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TrkB Antibody (OTI2E1)

Images

 
Western Blot: TrkB Antibody (2E1) [NBP1-47898] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY NTRK2 (RC205129, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: TrkB Antibody (2E1) [NBP1-47898] Staining of COS7 cells transiently transfected by pCMV6-ENTRY TrkB.
Immunohistochemistry-Paraffin: TrkB Antibody (2E1) [NBP1-47898] - Staining of paraffin-embedded Human lung tissue using anti-TrkB mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI2E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.17 mg/ml

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TrkB Antibody (OTI2E1) Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human NTRK2 expression vector
Specificity
This antibody is specific for Homo sapiens neurotrophic tyrosine kinase, receptor, type 2 (NTRK2), transcript variant b.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NTRK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:1000
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-47898 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.17 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TrkB Antibody (OTI2E1)

  • BDNF/NT-3 growth factors receptor
  • EC 2.7.10
  • EC 2.7.10.1
  • GP145-TrkB
  • Neurotrophic tyrosine kinase receptor type 2
  • neurotrophic tyrosine kinase, receptor, type 2
  • NTRK2
  • TrkB tyrosine kinase
  • TrkB
  • trk-B
  • TRKBBDNF-tropomyosine receptor kinase B
  • tyrosine kinase receptor B

Background

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternate transcriptional splice variants encoding different isoforms have been found for this gene

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for TrkB Antibody (NBP1-47898)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TrkB Antibody (NBP1-47898) (0)

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Secondary Antibodies

 

Isotype Controls

Additional TrkB Products

Research Areas for TrkB Antibody (NBP1-47898)

Find related products by research area.

Blogs on TrkB.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its ...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

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Bioinformatics

Gene Symbol NTRK2
Entrez