![Immunocytochemistry/Immunofluorescence: TRIM23 Antibody [NB100-78620] - HCT116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRIM23 protein stained by TRIM23 antibody [N1], N-term diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/TRIM23-Antibody-Immunocytochemistry-Immunofluorescence-NB100-78620-img0014.jpg "Immunocytochemistry/Immunofluorescence: TRIM23 Antibody [NB100-78620] - HCT116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRIM23 protein stained by TRIM23 antibody [N1], N-term diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.")
| Reactivity | Hu, Mu, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TRIM23. The exact sequence is proprietary. |
| Localization | Cell membrane |
| Predicted Species | Rhesus Macaque (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TRIM23 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 64 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
| Publication using NB100-78620 | Applications | Species |
|---|---|---|
| Kahle JJ, Gulbahce N, Shaw CA et al. Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia. Hum Mol Genet 2011-02-01 [PMID: 21078624] (ICC/IF, Mouse) | ICC/IF | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for TRIM23 Antibody (NB100-78620)Find related products by research area.
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![Immunohistochemistry-Paraffin: TRIM23 Antibody [NB100-78620] - Paraffin-embedded gastric CA, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/TRIM23-Antibody-Immunohistochemistry-Paraffin-NB100-78620-img0006.jpg "Immunohistochemistry-Paraffin: TRIM23 Antibody [NB100-78620] - Paraffin-embedded gastric CA, using antibody at 1:100 dilution.")
![Immunocytochemistry/Immunofluorescence: TRIM23 Antibody [NB100-78620] - Paraformaldehyde-fixed HeLa, using TRIM23 antibody (Green) at 1:500 dilution.](http://images.novusbio.com/fullsize/TRIM23-Antibody-Immunocytochemistry-Immunofluorescence-NB100-78620-img0008.jpg "Immunocytochemistry/Immunofluorescence: TRIM23 Antibody [NB100-78620] - Paraformaldehyde-fixed HeLa, using TRIM23 antibody (Green) at 1:500 dilution.")
![Western Blot: TRIM23 Antibody [NB100-78620] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM23 antibody [N1], N-term (NB100-78620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb100-78620_rabbit-polyclonal-trim23-antibody-910202420104455.jpg "Western Blot: TRIM23 Antibody [NB100-78620] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM23 antibody [N1], N-term (NB100-78620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")