Transglutaminase 2/TGM2 Assay Kit (Colorimetric)

The kit is shipped on ice. Upon arrival, the DTT (R1), the Reaction Buffer (R3) and Enzyme Tracer (R4) should be stored at -20C. All the other components of the kit should be kept at 4C. When stored properly, these stock solutions are stable for at least 24 months. OTHER SUPPLIES REQUIRED: - Recombinant human TG2 - TG2 inhibitor. This is recommended as negative control for screening of TG2 inhibitors.

Transglutaminases (EC. 2.3.213, R- glutamyl-peptide amine g-glutamyl-transferase) are a family of calcium dependent enzymes which catalyse an acyl transferase reaction between the gamma-carboxamide group of peptide bound glutamine and various primary amines. In mammals, at least eight active transglutaminase (TGs) isoenzymes have been described so far. They are widely distributed in various organs, tissues and body fluids. Among them, transglutaminase type 2 (TG2) is distinguished from others TGs by its functional versatility and ubiquitous expression pattern in mammalian tissues. This isoenzyme is involved in a variety of roles including stabilization of intra and extracellular matrices and crosslinking of cell envelopes in apoptosis. It has also been associated with a large number of pathological conditions such as fibrosis, celiac disease, neurodegenerative disorders, inflammatory processes in sepsis, and in carcinogenesis of hepatocellular and ovarian carcinoma. This consideration has led to the development of several tests for either research or clinical purpose. Among the various strategies developed to measure TG activity, the most sensitive and accurate quantification methods, although non-discriminatory, have been the radioactive and fluorimetric assays. Those methods detect the incorporation of labeled molecules, such as [3H/14C]-putrescine or monodansyl-cadaverine respectively, into glutamyl substrates such as casein or synthetic peptides. Solid phase assays developed so far in similar principles have been compromise by a high background signal, low sensitivity compare to radiolabeling methods and the lack of the specificity. This test kit overcomes these problems and constitutes a highly sensitive and specific TG2 solid-phase microassay. The TG2-Covtest uses a biotinylated preferred first substrate of TG2 (biotin-pepT26) as amine-acceptor, and spermine as second substrate (amine-donor) of the enzyme. Samples suspected of containing TG2 are incubated with calcium, dithiothreitol (DTT) and biotin-pepT26 in the wells of microtiter plates to which spermine has been covalently coupled. In the presence of TG2, spermine is incorporated into the g carboxamide of the glutaminyl residue of biotin-pepT26 to form a biotin-pepT26- g-glutamyl spermine. Enzymatic reaction is determined by its interaction with Streptavidin labelled peroxidase (SAv-HRP). Following a wash step to remove any unbound enzyme reagent, a substrate solution for SAv-HRP containing H2O2 and tetramethyl benzidine as electron acceptor (chromogen) is added and color developed. The color intensity is directly proportional to the TG2 activity in the sample.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

We have publications tested in 2 confirmed species: All, Porcine.

We have publications tested in 1 application: Func.

Showing Publications 1 - 9 of 9.

Publications using NBP1-37008	Applications	Species
Doki Y, Nakazawa Y, Sukegawa M et al. Piezo1 channel causes lens sclerosis via transglutaminase 2 activation Experimental eye research 2023-12-01 [PMID: 37951336]
Panes-Fernandez J, Godoy PA, Gavilan J et al. TG2 promotes amyloid beta aggregates: Impact on ER-mitochondria crosstalk, calcium homeostasis and synaptic function in Alzheimer's disease Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-03-27 [PMID: 36989728] (Func, All)	Func	All
Thomas K, Zondler L, Ludwig N et al. Glutamine prevents acute kidney injury by modulating oxidative stress and apoptosis in tubular epithelial cells JCI insight 2022-11-08 [PMID: 36107633] (Func, All)	Func	All
Han MS, Jung YK, Kim GW, Han S Transglutaminase-2 regulates Wnt and FoxO3a signaling to determine the severity of osteoarthritis Sci Rep 2020-08-06 [PMID: 32764573]
Chen C, Smith AD, Cheung L et al. Potentiation of IL-4 Signaling by Retinoic Acid in Intestinal Epithelial Cells and Macrophages-Mechanisms and Targets Front Immunol 2020-05-05 [PMID: 32431691] (Porcine)		Porcine
Jung HJ, Han EH, Jang IK et al. Effect of Tissue Transglutaminase on Steroid Resistance in T-Cell Acute Lymphoblastic Leukemia Anticancer Res. 2019-11-01 [PMID: 31704844]
Jung HJ, Chen Z, Wang M et al. Calcium blockers decrease the bortezomib resistance in mantle cell lymphoma via manipulation of tissue transglutaminase activities Blood 2012-03-15 [PMID: 22294726]
Perez Alea M, Kitamura M, Martin G, Thomas V, Hitomi K, El Alaoui S. Development of an isoenzyme-specific colorimetric assay for tissue transglutaminase 2 cross-linking activity. Anal Biochem. 389(2):150-6. 2009-06-15 [PMID: 19318081]
Thomas V, El Alaoui S, Massignon D, Clement S, Simonet F, Quash G. Development and evaluation of a modified colorimetric solid-phase microassay for measuring the activity of cellular and plasma (Factor XIII) transglutaminases. Biotechnol Appl Biochem. 43(Pt 3):171-9. 2006-03-01 [PMID: 16313237]

Array NBP1-37008

• Transglutaminase 2/TGM2 Antibodies
• Transglutaminase 2/TGM2 Kit
• Transglutaminase 2/TGM2 Lysates
• Transglutaminase 2/TGM2 Proteins
• Transglutaminase 2/TGM2 Proteins and Enzymes