![Immunocytochemistry/Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin protein stained by Transgelin antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Transgelin-TAGLN-SM22-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33003-img0015.jpg "Immunocytochemistry/Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin protein stained by Transgelin antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.")
| Reactivity | Hu, Mu, Rt, Po, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Transgelin/TAGLN/SM22 alpha. The exact sequence is proprietary. |
| Localization | Cytoplasm |
| Predicted Species | Porcine (98%), Bovine (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TAGLN |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Western_Blot_11082.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11144.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11480.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Mouse smooth muscle. Transgelin stained by Transgelin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Transgelin-TAGLN-SM22-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33003-img0020.jpg "Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Mouse smooth muscle. Transgelin stained by Transgelin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - 30 ug C8D30 whole cell lysate/extract 12% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Transgelin-TAGLN-SM22-alpha-Antibody-Western-Blot-NBP1-33003-img0006.jpg "Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - 30 ug C8D30 whole cell lysate/extract 12% SDS-PAGE gel, antibody dilution 1:1000.")
![Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - A. 30 ug Rat-2 whole cell lysate/extract 12 % SDS-PAGE Transgelin antibody dilution: 1:5000](http://images.novusbio.com/fullsize/Transgelin-TAGLN-SM22-alpha-Antibody-Western-Blot-NBP1-33003-img0010.jpg "Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - A. 30 ug Rat-2 whole cell lysate/extract 12 % SDS-PAGE Transgelin antibody dilution: 1:5000")
![Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (15 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody.](http://images.novusbio.com/fullsize/Transgelin-TAGLN-SM22-alpha-Antibody-Western-Blot-NBP1-33003-img0017.jpg "Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (15 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody.")
![Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Rat stomach. Transgelin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Transgelin-TAGLN-SM22-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33003-img0014.jpg "Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Rat stomach. Transgelin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33003_rabbit-polyclonal-transgelin-tagln-sm22-alpha-antibody-western-blot-304202410615..jpg "Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.")
![Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin/TAGLN/SM22 alpha antibody (NBP1-33003) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33003_rabbit-polyclonal-transgelin-tagln-sm22-alpha-antibody-910202420122029.jpg "Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin/TAGLN/SM22 alpha antibody (NBP1-33003) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot Myocardin Antibody (355521) [Unconjugated]](https://images.novusbio.com/images2/Myocardin_MAB4028_Western_Blot_6147.jpg)
![Western Blot Myocardin Antibody (355521) [Unconjugated]](https://images.novusbio.com/images/mab4028_human-mouse-myocardin-mab-clone-355521-412024125217.jpg)
![Western Blot Myocardin Antibody (355521) [Unconjugated]](https://images.novusbio.com/images/mab4028_human-mouse-myocardin-mab-clone-355521-41202412505317.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")