Recombinant E. coli Thioredoxin Reductase 1/TRXR1 Protein Summary
Description |
An un-tagged recombinant protein corresponding to the amino acids 1-321 of E.coli Thioredoxin Reductase 1/TRXR1 Source: E.coli Amino Acid Sequence: MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K |
Details of Functionality |
Specific activity is > 10 units/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm. |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
TXNRD1 |
Purity |
>90%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
34.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
>90%, by SDS-PAGE |
Alternate Names for Recombinant E. coli Thioredoxin Reductase 1/TRXR1 Protein
Background
TRXB(Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E.coli TRXB protein was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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