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TCP1-eta Antibody

Images

 
Western Blot: TCP1-eta Antibody [NBP1-88144] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CCT7 antibody. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: TCP1-eta Antibody [NBP1-88144] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TCP1-eta Antibody [NBP1-88144] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Western Blot: TCP1-eta Antibody [NBP1-88144] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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TCP1-eta Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ELKAEKDNAEIRVHTVEDYQAIVDAEWNILYDKLEKIHHSGAKVVLSKLPIGDVATQYFADRDMFCAGRVPEEDLKRTMMACGGSIQTSVNALSADVLGRCQVFEETQIGGERYNFFTGCPKAKTCTFILRGGAEQFMEETE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCT7
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TCP1-eta Protein (NBP1-88144PEP)
Publications
Read Publications using NBP1-88144.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24213632)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TCP1-eta Antibody

  • CCTETA
  • CCT-eta
  • CCTH
  • chaperonin containing TCP1, subunit 7 (eta)
  • eta subunit
  • HIV-1 Nef interacting protein
  • HIV-1 Nef-interacting protein
  • MGC110985
  • Nip7-1
  • T-complex protein 1 subunit eta

Background

TCP1-eta encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants encoding different isoforms have been found for this gene, but only two of them have been characterized to date.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TCP1-eta Antibody (NBP1-88144)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TCP1-eta Antibody (NBP1-88144) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for TCP1-eta Antibody (NBP1-88144)

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Blogs on TCP1-eta

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Bioinformatics

Gene Symbol CCT7