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TBX3 Antibody [Unconjugated]

 (2 Publications)
 (1 Review)
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TBX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4509) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for TBX3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TBX3 Antigen Affinity-purified ...read more
Western blot shows lysates of LNCaP human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TBX3 Antigen Affinity-purified Polyclonal ...read more
TBX3 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Human TBX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4509) at 5 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF4509-SP
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TBX3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human TBX3
Ser321-Thr483
Accession # O15119
Specificity
Detects human TBX3 in direct ELISAs and Western blots.  In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TBX2, rhTBX6, and rhTBX20 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TBX3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1-2 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF4509 in the following applications:
Publications
Read Publications using
AF4509 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TBX3 Antibody [Unconjugated]

  • bladder cancer related protein XHL
  • T-box 3
  • T-box protein 3
  • T-box transcription factor TBX3
  • TBX3
  • TBX3-ISO
  • ulnar mammary syndrome
  • UMS
  • XHL

Background

TBX3 belongs to the T-box family of transcription factors, which are involved in regulation of developmental processes. TBX3 acts as a transcriptional repressor regulating anterior/posterior axis of the forelimb. Mutations in TBX3 are associated with ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. TBX3 has 3 splice isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TBX3 Antibody
[Unconjugated]
AF4509
TBX3 Antibody [Unconjugated]
Species: Hu
Applications: WB, ICC/IF

Publications for TBX3 Antibody (AF4509)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Human
(1)
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Review for TBX3 Antibody (AF4509) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF4509:
Filter by Applications
ICC
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry TBX3 AF4509
Enlarge 		5
reviewed by:
Verified Customer
 ICC Mouse 08/04/2016
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Summary

ApplicationImmunocytochemistry
Sample TestedR1 wild type Mouse embryonic Stem Cells
SpeciesMouse
LotZRG0215071

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Recent Reviews

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Immunocytochemistry TBX3 AF4509
Enlarge
 Verified Customer
08/04/2016
Application: ICC
Species: Mouse
read this review

Bioinformatics

Gene Symbol TBX3
Uniprot