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TBX2 Antibody (393725)

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Western blot shows lysates of HEK293T human embryonic kidney cell line, JEG-3 human epithelial choriocarcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, and BG0IV human embryonic stem cells. PVDF membrane was probed with 0.1 µg/mL of Human TBX2 Monoclonal Antibody (Catalog # MAB5040) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TBX2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HEK293T human embryonic kidney cell line, JEG-3 human epithelial choriocarcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, and BG0IV human embryonic stem cells. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
393725
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5040-SP
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TBX2 Antibody (393725) Summary

Immunogen
E. coli-derived recombinant human TBX2
Thr593-Arg702
Accession # Q13207
Specificity
Detects endogenous human TBX2 in Western blots at approximately 75 kDa.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
TBX2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TBX2 Antibody (393725)

  • FLJ10169
  • T-box 2
  • T-box protein 2
  • T-box transcription factor TBX2
  • TBX2

Background

TBX2 (T-box transcription factor 2) is a member of a family of transcription factors that share the T-box DNA binding domain. TBX2, as well as other TBX proteins, is involved in remodeling of the heart myocardium through a complex spatial and temporal regulation. TBX2 is associated with tumorigenesis and anti-senescence properties by affecting several cyclin-dependent kinase inhibitors. The full-length protein contains 702 amino acids with a DNA binding domain from aa 99 to aa 277.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TBX2 Antibody
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Bioinformatics

Gene Symbol TBX2
Uniprot