![Immunocytochemistry/Immunofluorescence: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SURF5 Antibody (PCRP-MED22-1E4) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.](http://images.novusbio.com/fullsize/SURF5-Antibody-PCRP-MED22-1E4-Immunocytochemistry-Immunofluorescence-NBP3-13787-img0002.jpg "Immunocytochemistry/Immunofluorescence: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SURF5 Antibody (PCRP-MED22-1E4) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IP, MA |
| Clone | PCRP-MED22-1E4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14190) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant full-length human SURF5 protein (Uniprot: Q15528) |
| Localization | Nucleus |
| Isotype | IgG2c |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MED22 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | ELISA: For coating, order antibody without BSA Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate) |
| Theoretical MW | 22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Flow Cytometry: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787] - Flow cytometric analysis of PFA-fixed HeLa cells. SURF5 antibody (PCRP-MED22-1E4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/SURF5-Antibody-PCRP-MED22-1E4-Flow-Cytometry-NBP3-13787-img0001.jpg "Flow Cytometry: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787] - Flow cytometric analysis of PFA-fixed HeLa cells. SURF5 antibody (PCRP-MED22-1E4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).")
![Protein Array: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SURF5 Antibody (PCRP-MED22-1E4).](http://images.novusbio.com/fullsize/SURF5-Antibody-PCRP-MED22-1E4-Protein-Array-NBP3-13787-img0004.jpg "Protein Array: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SURF5 Antibody (PCRP-MED22-1E4).")
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