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SUPT16H Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: SUPT16H Antibody [NBP2-38607] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: SUPT16H Antibody [NBP2-38607] - Staining in human testis and skeletal muscle tissues using anti-SUPT16H antibody. Corresponding SUPT16H RNA-seq data are ...read more
Immunohistochemistry-Paraffin: SUPT16H Antibody [NBP2-38607] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: SUPT16H Antibody [NBP2-38607] - Staining of human testis shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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SUPT16H Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ASITSEVFNKFFKERVMEIVDADEKVRHSKLAESVEKAIEEKKYLAGADPSTVEMCYPPIIQSGGNYNLKFSVVSDKNHMHFGAITCAMGIRFKSY
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SUPT16H
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SUPT16H Protein (NBP2-38607PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SUPT16H Antibody

  • CDC68
  • Chromatin-specific transcription elongation factor 140 kDa subunit
  • facilitates chromatin remodeling 140 kDa subunit
  • Facilitates chromatin transcription complex subunit SPT16
  • FACT 140 kDa subunit
  • FACT complex subunit SPT16
  • FACT140
  • FACTp140
  • FACTP140FACT
  • FLJ10857
  • FLJ14010
  • FLJ34357
  • hSPT16
  • SPT16/CDC68
  • suppressor of Ty (S.cerevisiae) 16 homolog
  • suppressor of Ty 16 homolog (S. cerevisiae)

Background

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SUPT16H
Uniprot