Immunocytochemistry/ Immunofluorescence: SUCNR1/GPR91 Antibody [NBP1-00861] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Orthogonal Strategies: Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - D) SUCNR1 protein expression relative to B-tubulin in vitro. Data are shown as mean (+/- SEM) of n >= 3 independent replicates per ...read more
Immunohistochemistry-Paraffin: SUCNR1/GPR91 Antibody [NBP1-00861] - Staining of GPR91 in mouse Vas Deferens where strong membrane staining is observed.
Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Analysis of human kidney tissue (A), mouse kidney tissue (B), rat kidney tissue (C), and hek293 cells (D) using GPR91 antibody at 2ug/ml.
Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Analysis of GPR91 (A135) antibody in extracts from HUVEC/MCF-7 cells.
Immunocytochemistry/ Immunofluorescence: SUCNR1/GPR91 Antibody [NBP1-00861] - GPR91 antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
The absolute number of LC is increased in K14E7 mouse epidermis.Sections of C57Bl/6 (A) and K14E7 (B) mouse ear were stained with CD207 and imaged by epifluorescence microscopy. The epidermis (top) is indicated by a ...read more
In vitro assay reported in scientific literature (PMID 30478422)
Western Blot 1-2 ug/ml
Theoretical MW
38 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Alternate Names for SUCNR1/GPR91 Antibody - BSA Free
GPR91
GPR91G protein-coupled receptor 91
G-protein coupled receptor 91
P2Y purinoceptor 1
P2Y purinoceptor 1-like
succinate receptor 1
SUCNR1
Background
GPR91 (formerly known as P2U2) is a G protein-coupled, dicarboxylic acid succinate receptor with the highest level of expression in the kidney, predominantly in the proximal tubules and the lowest in the liver and the spleen. GPR91 functions as a citric acid cycle intermediate succinate receptor. Two signaling pathways result from GPR91 activation, the pertussis-toxin-sensitive Gi/Go pathway and the pertussis-toxin-insensitive Gq pathway. Four amino acid residues are necessary for GPR91 activation by succinate: Arg 99, His 103, Arg 252 and Arg 281. GPR91 plays an important role in the succinate-induced hypertensive effect and may be involved in renovascular hypertension, a disease linked to diabetes, renal failure and atherosclerosis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Pu M, Zhang J, Hong F et al. The pathogenic role of Succinate-SUCNR1: A critical function that induces renal fibrosis via infiltration of M2 macrophage Research Square 2023-08-21 (WB, Mouse)
FAQs for SUCNR1/GPR91 Antibody (NBP1-00861). (Showing 1 - 1 of 1 FAQs).
I am looking for a reliable GPR91 antibody suitable for immunohistochemistry and FACS. I know the Saphieia group from Montreal who used your GPR91 antibody in the past. Sadly they told me that that particular antibody is no longer available. Please can you advise me whether the new antibody NBP1-00861 truly does work for IHC/IF.
Yes, a couple of our other GPR91 antibodies were discontinued last year and at the moment we have only one GPR91 antibody (#NBP1-00861) which is one of our best selling products without even a single complaint. In our lab, we have tested GPR91-Antibody NBP1-00861 for WB and ICC/IF applications in Human, Mouse & Rat species. We have not tested this antibody in IHC and FLOW applications yet. However, some of our customers have used this antibody for IHC application and you may see following publications for that: Vargas et al. J Am Soc Nephrol. 2009 May;20(5):1002-11; Robben et al. Kidney Int. 2009 Dec;76(12):1258-67. Moreover, Hu et al (Exp Eye Res. 2013 Apr;109:31-9) and Aguiar et al. (Cell Calcium. 2010 Jan;47(1):37-46) have cited the ICC-IF use of this product. Because this antibody worked well in ICC-IF application and it is compatible with IF based immunodetection methods, I strongly believe that this antibody should work in FLOW application also. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.
SUCNR1/GPR91 - a potential role in renovascular hypertension SUCNR1 is the cognate receptor for the Kreb's citric acid cycle intermediate succinate. It is of interest to scientists because it is involved in not only energy metabolism but possibly also in renovascular hypertension, a condition linked to diabe... Read full blog post.
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![Immunocytochemistry/Immunofluorescence: SUCNR1/GPR91 Antibody [NBP1-00861] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SUCNR1/GPR91 Antibody NBP1-00861 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00861-img0019.jpg "Immunocytochemistry/Immunofluorescence: SUCNR1/GPR91 Antibody [NBP1-00861] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SUCNR1/GPR91 Antibody NBP1-00861 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
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![Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - D) SUCNR1 protein expression relative to B-tubulin in vitro. Data are shown as mean (+/- SEM) of n >= 3 independent replicates per condition. (H) qRT-PCR of SUCNR1 basal expression in human cells. Data are normalized on 18S and expressed as mean fold change (+/- SEM) versus NSCs from n >= 3 independent replicates per condition. (I) Representative blot of SUNCR1 basal protein expression in human cells. hBJFs, human BJ fibroblasts. *p <= 0.05 versus 0'. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29478844) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Western-Blot-NBP1-00861-img0020.jpg "Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - D) SUCNR1 protein expression relative to B-tubulin in vitro. Data are shown as mean (+/- SEM) of n >= 3 independent replicates per condition. (H) qRT-PCR of SUCNR1 basal expression in human cells. Data are normalized on 18S and expressed as mean fold change (+/- SEM) versus NSCs from n >= 3 independent replicates per condition. (I) Representative blot of SUNCR1 basal protein expression in human cells. hBJFs, human BJ fibroblasts. *p <= 0.05 versus 0'. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29478844) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: SUCNR1/GPR91 Antibody [NBP1-00861] - Staining of GPR91 in mouse Vas Deferens where strong membrane staining is observed.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Immunohistochemistry-Paraffin-NBP1-00861-img0015.jpg "Immunohistochemistry-Paraffin: SUCNR1/GPR91 Antibody [NBP1-00861] - Staining of GPR91 in mouse Vas Deferens where strong membrane staining is observed.")
![Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Analysis of human kidney tissue (A), mouse kidney tissue (B), rat kidney tissue (C), and hek293 cells (D) using GPR91 antibody at 2ug/ml.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Western-Blot-NBP1-00861-img0016.jpg "Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Analysis of human kidney tissue (A), mouse kidney tissue (B), rat kidney tissue (C), and hek293 cells (D) using GPR91 antibody at 2ug/ml.")
![Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Lane1:Hela whole cell lysate. Lane2:Mouse kidney tissue lysate. Lane3:Rat kidney tissue lysate.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Western-Blot-NBP1-00861-img0017.jpg "Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Lane1:Hela whole cell lysate. Lane2:Mouse kidney tissue lysate. Lane3:Rat kidney tissue lysate.")
![Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Analysis of GPR91 (A135) antibody in extracts from HUVEC/MCF-7 cells.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Western-Blot-NBP1-00861-img0018.jpg "Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Analysis of GPR91 (A135) antibody in extracts from HUVEC/MCF-7 cells.")
![Immunocytochemistry/Immunofluorescence: SUCNR1/GPR91 Antibody [NBP1-00861] - GPR91 antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00861-img0014.jpg "Immunocytochemistry/Immunofluorescence: SUCNR1/GPR91 Antibody [NBP1-00861] - GPR91 antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")