![Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) [NBP3-07770] - Formalin-fixed, paraffin-embedded normal human spleen tissue stained with ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Mouse Monoclonal Antibody (ZB55).](http://images.novusbio.com/fullsize/ST6-Gal-Sialyltransferase-1-ST6GAL1-CD75-Antibody-ZB55-Immunohistochemistry-Paraffin-NBP3-07770-img0002.jpg "Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) [NBP3-07770] - Formalin-fixed, paraffin-embedded normal human spleen tissue stained with ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Mouse Monoclonal Antibody (ZB55).")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clone | ZB55 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08436) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Additional Information | Clone ZB55 was used by HLDA to establish CD designation. |
| Immunogen | ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 peptides (Uniprot: P15907) |
| Localization | Cell surface and cytoplasmic |
| Marker | B-Cell Marker |
| Specificity | Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This monoclonal antibody reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues. |
| Isotype | IgM Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ST6GAL1 |
| Purity | PEG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 46 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | PEG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (NBP3-07770)Find related products by research area.
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![Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) [NBP3-07770] - Formalin-fixed, paraffin-embedded normal human spleen tissue stained with ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Mouse Monoclonal Antibody (ZB55).](http://images.novusbio.com/fullsize/ST6-Gal-Sialyltransferase-1-ST6GAL1-CD75-Antibody-ZB55-Immunohistochemistry-Paraffin-NBP3-07770-img0001.jpg "Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) [NBP3-07770] - Formalin-fixed, paraffin-embedded normal human spleen tissue stained with ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Mouse Monoclonal Antibody (ZB55).")
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