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SSSCA1 Antibody (OTI2F5) - Azide and BSA Free

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Western Blot: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSSCA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSSCA1.
Immunohistochemistry: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SSSCA1 mouse monoclonal antibody.
Flow Cytometry: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - Analysis of Jurkat cells, using anti-SSSCA1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SSSCA1 mouse monoclonal antibody.
Immunohistochemistry: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SSSCA1 mouse monoclonal antibody.
Immunohistochemistry: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SSSCA1 mouse monoclonal antibody.
Immunohistochemistry: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSSCA1 mouse monoclonal antibody.
Immunohistochemistry: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SSSCA1 mouse monoclonal antibody.
Immunohistochemistry: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SSSCA1 mouse monoclonal antibody.
Immunohistochemistry: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SSSCA1 mouse monoclonal antibody.
Flow Cytometry: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - Analysis of Hela cells, using anti-SSSCA1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SSSCA1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI2F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

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SSSCA1 Antibody (OTI2F5) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human SSSCA1 (NP_006387) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ZNRD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
21.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SSSCA1 Antibody (OTI2F5) - Azide and BSA Free

  • Autoantigen p27
  • centromeric autoantigen (27kD)
  • p27
  • Sjoegren syndrome/scleroderma autoantigen 1
  • Sjogren syndrome/scleroderma autoantigen 1
  • Sjogren's syndrome/scleroderma autoantigen 1

Background

This antigen is recognized by a subset of anti-centromere antibodies from patients with scleroderma and/or Sjogren's syndrome. Subcellular localization has not yet been established. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ZNRD2