![Western Blot: SRGAP2 Antibody [NBP1-49877] - (0.1ug/ml) staining of Rat Cortical Neuron lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Ms. Ya-Jing Mi and Dr.Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong Univer](http://images.novusbio.com/fullsize/SRGAP2-Antibody-Western-Blot-NBP1-49877-img0002.jpg "Western Blot: SRGAP2 Antibody [NBP1-49877] - (0.1ug/ml) staining of Rat Cortical Neuron lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Ms. Ya-Jing Mi and Dr.Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong Univer")
| Reactivity | Hu, Mu, Rt, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-KQEDRQTPRSPDST corresponding to internal region according to NP_056141.2. |
| Epitope | KQEDRQTPRSPDST - Internal region |
| Specificity | This antibody is expected to recognize isoform a (NP_056141.2). |
| Predicted Species | Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | SRGAP2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | IF: Transfected HEK293 transiently expressing GFP-tagged human srGAP2 with the primary antibody stained in the red channel (Confocal IF). WB: Approx. 124 kDa band observed in HeLa lysate and in rat primary culture cortical neurons at Day 4 (calculated MW of 121 kDa band according to human NP_056141.2, but the rat protein sequence is stlll provisional). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | SRGAP2 |
| Entrez | |
| Uniprot |
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![Immunocytochemistry/Immunofluorescence: SRGAP2 Antibody [NBP1-49877] - HEK293 overexpressing Human srGAP2 and probed with EB09775 at 2.5ug/ml in the right panel. Data kindly provided by Ms. Ya-Jing Mi and Dr.Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong University.](http://images.novusbio.com/fullsize/SRGAP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49877-img0001.jpg "Immunocytochemistry/Immunofluorescence: SRGAP2 Antibody [NBP1-49877] - HEK293 overexpressing Human srGAP2 and probed with EB09775 at 2.5ug/ml in the right panel. Data kindly provided by Ms. Ya-Jing Mi and Dr.Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong University.")
