Home » SPNS1 » SPNS1 Lysates » SPNS1 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

SPNS1 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: SPNS1 Overexpression Lysate (Adult Normal) [NBP2-09645] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPNS1.
Western Blot: SPNS1 Overexpression Lysate (Adult Normal) [NBP2-09645] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPNS1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals offers 100% Guarantee

SPNS1 Overexpression Lysate Summary

Description

SPNS1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC227635

Accession#: NM_001142448

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
SPNS1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
56.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for SPNS1 Overexpression Lysate

  • FLJ38358
  • HSpin1Spinster-like protein 1
  • LAT
  • PP2030
  • protein spinster homolog 1
  • SPIN1nrs
  • SPINL
  • spinster homolog 1 (Drosophila)

Background

SPNS1 is a human ortholog of the Drosophila melanogaster spin gene. Many orthologs have been identified in a number of species from nematodes to humans. In drosophila the Spin protein may function in mediating apoptotic signals conveyed from follicle cells to nurse cells or from glia to neurons. The accumulation of lipofuscin-like materials in spin mutant neurons further implies the possible function of the spin gene in regulating lysosomal turnover in nerve cells. SPNS1 may be involved in necrotic or autophagic cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

ZAP70 Antibody -
BSA Free
NBP1-87000
Western Blot ZAP70 Antibody - BSA Free
Immunohistochemistry-Paraffin ZAP70 Antibody - BSA Free
Immunohistochemistry-Paraffin ZAP70 Antibody - BSA Free
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
SLP-76/LCP2 [p
Tyr145] Antibody (776 ...
MAB7474
Simple Western SLP-76/LCP2 [p Tyr145] Antibody (776503) [Unconjugated]
Western Blot SLP-76/LCP2 [p Tyr145] Antibody (776503) [Unconjugated]
Immunocytochemistry SLP-76/LCP2 [p Tyr145] Antibody (776503) [Unconjugated]
Species: Hu
Applications: ICC, Simple Western, WB
GRAP2 Antibody
(OTI1G2)
NBP2-00764
Flow Cytometry GRAP2 Antibody (OTI1G2)
Flow Cytometry GRAP2 Antibody (OTI1G2)
Western Blot GRAP2 Antibody (OTI1G2)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
SYK Antibody - BSA
Free
NBP1-32945
Western Blot SYK Antibody - BSA Free
Western Blot SYK Antibody - BSA Free
Immunoprecipitation SYK Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
Lck [p Tyr394]
Antibody (755103)
MAB7500
Immunocytochemistry Lck [p Tyr394] Antibody (755103)
Western Blot Lck [p Tyr394] Antibody (755103)
(11 Publications)
Species: Hu
Applications: ICC, WB
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(379 Publications)
Species: Hu
Applications: BA
NTAL Antibody
[Unconjugated]
AF4066
Western Blot NTAL Antibody [Unconjugated]
Intracellular Staining by Flow Cytometry NTAL Antibody [Unconjugated]
Species: Hu
Applications: CyTOF-ready, ICFlow, WB
CD3 Antibody (SP7)
NB600-1441
Immunohistochemistry-Frozen CD3 Antibody (SP7)
Immunohistochemistry-Paraffin CD3 Antibody (SP7)
Immunohistochemistry-Paraffin CD3 Antibody (SP7)
(3 Reviews)
(49 Publications)
Species: Ca, Hu, Mu, Po
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KD
Src [p Tyr419]
Antibody [Unconjugate
AF2685
Western Blot Src [p Tyr419] Antibody [Unconjugated]
Simple Western Src [p Tyr419] Antibody [Unconjugated]
Immunohistochemistry Src [p Tyr419] Antibody [Unconjugated]
(1 Review)
(10 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
GRB2 Antibody
[Unconjugated]
AF3846
Western Blot GRB2 Antibody [Unconjugated]
Immunohistochemistry GRB2 Antibody [Unconjugated]
(2 Publications)
Species: Hu, Mu, Rt
Applications: IHC, WB
Vav-1 [p Tyr160]
Antibody (751411) [Un ...
MAB37861
Simple Western Vav-1 [p Tyr160] Antibody (751411) [Unconjugated]
Western Blot Vav-1 [p Tyr160] Antibody (751411) [Unconjugated]
Immunocytochemistry Vav-1 [p Tyr160] Antibody (751411) [Unconjugated]
(1 Publication)
Species: Hu
Applications: ICC, Simple Western, WB
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(52 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
EphB2 Antibody
[Unconjugated]
AF467
Simple Western EphB2 Antibody [Unconjugated]
Western Blot EphB2 Antibody [Unconjugated]
Immunocytochemistry EphB2 Antibody [Unconjugated]
(5 Reviews)
(63 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
CD8 Antibody
(53-6.7) - BSA Free
NBP1-49045
Immunohistochemistry-Paraffin CD8 Antibody (53-6.7) - BSA Free
Immunocytochemistry/ Immunofluorescence CD8 Antibody (53-6.7) - BSA Free
Immunohistochemistry-Frozen CD8 Antibody (53-6.7) - BSA Free
(3 Reviews)
(28 Publications)
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
Thyroglobulin
Antibody (2H11 + 6E1)
NBP2-34294
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
(3 Reviews)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P

Publications for SPNS1 Lysate (NBP2-09645) (0)

There are no publications for SPNS1 Lysate (NBP2-09645).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SPNS1 Lysate (NBP2-09645) (0)

There are no reviews for SPNS1 Lysate (NBP2-09645). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SPNS1 Lysate (NBP2-09645) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SPNS1 Products

Research Areas for SPNS1 Lysate (NBP2-09645)

Find related products by research area.

Blogs on SPNS1

There are no specific blogs for SPNS1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

As an alternative, please consider our next generation Recombinant Human IL-2 (BT-002). It has equivalent bioactivity to Recombinant Human IL-2 (Catalog # 202-IL). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human IL-2 proteins are measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells.
IL-2 [Unconjugated]
202-IL-010

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SPNS1 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol SPNS1