Home » Sonic Hedgehog/Shh » Sonic Hedgehog/Shh Antibodies » Sonic Hedgehog/Shh Antibody (171001) [Unconjugated] - N-Terminus
Novus Biologicals products are now on bio-techne.com

Sonic Hedgehog/Shh Antibody (171001) [Unconjugated] - N-Terminus

 (4 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB464) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB464) followed by ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
171001
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB464-SP
Novus Biologicals offers 100% Guarantee

Sonic Hedgehog/Shh Antibody (171001) [Unconjugated] - N-Terminus Summary

Immunogen
E. coli-derived recombinant mouse Sonic Hedgehog
Cys25-Gly198 (Lys122Arg)
Accession # Q62226
Specificity
Detects the N-terminal peptide of human (amino acids 24-197) and mouse (amino acids 25-198) Shh in direct ELISAs and Western blots. Shows 50-100% cross-reactivity with the N-terminal peptides of recombinant mouse (rm) Dhh (amino acids 23‑198) and rmIhh (amino acids 66‑240) and no cross-reactivity with the C‑terminal peptides from rmShh (amino acids 199‑437), rmDhh (amino acids 199‑396), or rmIhh (amino acids 241‑449).
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
SHH
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB464 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sonic Hedgehog/Shh Antibody (171001) [Unconjugated] - N-Terminus

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • ShhNC
  • SMMCI
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT
  • TPTPS

Background

Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

GLI-1 Antibody -
BSA Free
NB600-600
Immunohistochemistry-Paraffin GLI-1 Antibody - BSA Free
Immunohistochemistry GLI-1 Antibody - BSA Free
Immunohistochemistry-Paraffin GLI-1 Antibody - BSA Free
(1 Review)
(57 Publications)
Species: Ca, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, IB, ICC/IF, IHC, IHC-P, KD, WB
Patched 1/PTCH
Antibody (413220) [Un ...
MAB41051
Immunohistochemistry Patched 1/PTCH Antibody (413220) [Unconjugated] - (First Extracellular Loop)
Western Blot Patched 1/PTCH Antibody (413220) [Unconjugated] - (First Extracellular Loop)
Immunohistochemistry Patched 1/PTCH Antibody (413220) [Unconjugated] - (First Extracellular Loop)
(10 Publications)
Species: Hu, Mu
Applications: IHC, WB
BMP-4
[Unconjugated]
314-BP
SDS-PAGE BMP-4 [Unconjugated]
Bioactivity BMP-4 [Unconjugated]
(530 Publications)
Species: Hu
Applications: BA, BA
Fucosyltransferase
1/FUT1 Antibody
NBP3-35576
Western Blot Fucosyltransferase 1/FUT1 Antibody
Species: Hu, Mu, Rt
Applications: ELISA, WB
HFE Antibody
NBP1-83250
Western Blot HFE Antibody
Immunohistochemistry HFE Antibody
Immunohistochemistry-Paraffin HFE Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
FGF-8
[Unconjugated]
423-F8
SDS-PAGE FGF-8 [Unconjugated]
Bioactivity FGF-8 [Unconjugated]
Cell Culture FGF-8 [Unconjugated]
(1 Review)
(63 Publications)
Species: Hu, Mu
Applications: BA
Smoothened
Antibody - BSA Free
NLS2666
Immunohistochemistry-Paraffin Smoothened Antibody - BSA Free
(1 Publication)
Species: Bt, Bv, Ca, Eq, Ha, Hu, Pm, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-P
SMOX Antibody -
Azide and BSA Free
NBP3-04509
Immunohistochemistry-Paraffin SMOX Antibody - Azide and BSA Free
Western Blot SMOX Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin SMOX Antibody - Azide and BSA Free
(1 Publication)
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
GLI-3 Antibody
AF3690
Western Blot GLI-3 Antibody
Chromatin Immunoprecipitation (ChIP) GLI-3 Antibody
Immunocytochemistry GLI-3 Antibody
(3 Reviews)
(102 Publications)
Species: Hu, Mu
Applications: ChIP, ICC, WB
GLI-2 Antibody
AF3635
Immunohistochemistry GLI-2 Antibody
(2 Reviews)
(44 Publications)
Species: Mu
Applications: IHC, WB
Indian
Hedgehog/Ihh Antibody ...
AF1705
Immunohistochemistry Indian Hedgehog/Ihh Antibody - N-Terminus
(3 Publications)
Species: Mu
Applications: IHC, WB
BMP-2
[Unconjugated]
355-BM
Bioactivity BMP-2 [Unconjugated]
(191 Publications)
Species: Hu, Mu, Rt
Applications: BA
Factor IX Antibody
NBP1-33581
Immunohistochemistry-Paraffin Factor IX Antibody
Immunohistochemistry-Paraffin Factor IX Antibody
Immunocytochemistry/ Immunofluorescence Factor IX Antibody
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P
Ret Antibody
[Unconjugated]
AF482
Immunohistochemistry Ret Antibody [Unconjugated]
Western Blot Ret Antibody [Unconjugated]
Immunohistochemistry Ret Antibody [Unconjugated]
(40 Publications)
Species: Mu
Applications: IHC, WB
CCDC6 Antibody
NBP1-85351
Immunohistochemistry-Paraffin CCDC6 Antibody
Immunohistochemistry-Paraffin CCDC6 Antibody
Immunohistochemistry-Paraffin CCDC6 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
TAS2R38 Antibody
NBP3-41370
Immunohistochemistry-Paraffin TAS2R38 Antibody
Species: Hu, Po
Applications: ICC/IF, IHC, WB
FGF-4
[Unconjugated]
235-F4
SDS-PAGE FGF-4 [Unconjugated]
Bioactivity FGF-4 [Unconjugated]
(76 Publications)
Species: Hu
Applications: BA
FGF-10
[Unconjugated]
345-FG
SDS-PAGE FGF-10 [Unconjugated]
Bioactivity FGF-10 [Unconjugated]
Bioactivity FGF-10 [Unconjugated]
(103 Publications)
Species: Hu
Applications: BA

Publications for Sonic Hedgehog/Shh Antibody (MAB464)(4)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Rat
(1)
All Species

Reviews for Sonic Hedgehog/Shh Antibody (MAB464) (0)

There are no reviews for Sonic Hedgehog/Shh Antibody (MAB464). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Sonic Hedgehog/Shh Antibody (MAB464) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Sonic Hedgehog/Shh Products

Blogs on Sonic Hedgehog/Shh

There are no specific blogs for Sonic Hedgehog/Shh, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Sonic Hedgehog/Shh Antibody (171001) [Unconjugated] - N-Terminus and receive a gift card or discount.


Bioinformatics

Gene Symbol SHH
Uniprot