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Somatostatin R2/SSTR2 Antibody (402038) [Unconjugated]

 (14 Publications)
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Somatostatin R2/SSTR2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using 10 µg/mL Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (brown; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Somatostatin R2/SSTR2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using 10 µg/mL Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224) for 3 hours at room temperature. ...read more
MDA‑MB‑231 human breast cancer cell line was stained with Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), ...read more
Somatostatin R2/ SSTR2 was detected in immersion fixed paraffin-embedded sections of human brain (cingulate cortex) using 3 µg/mL Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224) overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, CyTOF-ready, Flow, ICC/IF
Clone
402038
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC4224U-100UG
Alexa Fluor 350
FAB4224U
Alexa Fluor 405
IC4224V-100UG
Alexa Fluor 488
IC4224G-100UG
Alexa Fluor 488
FAB4224G
Alexa Fluor 532
FAB4224X
Alexa Fluor 594
IC4224T-100UG
Alexa Fluor 594
FAB4224T
Alexa Fluor 647
IC4224R-100UG
Alexa Fluor 700
IC4224N-100UG
Alexa Fluor 700
FAB4224N
Alexa Fluor 750
FAB4224S
Alexa Fluor 750
IC4224S-100UG
Allophycocyanin
FAB4224A
Biotin
FAB4224B
DyLight 350
FAB4224D
DyLight 405
FAB4224E
DyLight 488
FAB4224K
DyLight 550
FAB4224L
DyLight 594
FAB4224M
DyLight 650
FAB4224W
DyLight 680
FAB4224Y
DyLight 755
FAB4224Z
FITC
FAB4224F
HRP
FAB4224H
Janelia Fluor 549
FAB4224I
Janelia Fluor 646
FAB4224J
PerCP
FAB4224C
Phycoerythrin
FAB4224P
mFluor Violet 450 SE
FAB4224MFV450
mFluor Violet 500 SE
FAB4224MFV500
mFluor Violet 610 SE
FAB4224MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB4224A
Carrier Free
FAB4224P
Carrier Free
MAB4224-SP
Carrier Free
IC4224V-100UG
Carrier Free
IC4224U-100UG
Carrier Free
IC4224T-100UG
Carrier Free
IC4224S-100UG
Carrier Free
IC4224R-100UG
Carrier Free
IC4224N-100UG
Carrier Free
IC4224G-100UG
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Somatostatin R2/SSTR2 Antibody (402038) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with Somatostatin R2/SSTR2
Met1-Ile369
Accession # P30874
Specificity
Detects human Somatostatin R2/SSTR2. Stains SSTR2 transfectants but not irrelevant transfectants.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SSTR2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publications using
MAB4224 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Somatostatin R2/SSTR2 Antibody (402038) [Unconjugated]

  • Smstr2
  • Somatostatin R2
  • somatostatin receptor 2
  • somatostatin receptor type 2
  • SomatostatinR2
  • SRIF-1
  • SS2R
  • SS-2-R
  • SS2-R
  • SST2
  • SSTR2

Background

Somatostatin Receptor 2 (SSTR2) is one of five 7-transmembrane G-protein-coupled receptors for somatostatins 14 and 28. Human SSTR2 shares 84% aa identity with mouse SSTR2 within the extracellular domains. Isoform B (357 aa) has an alternate C-terminal cytoplasmic region that is 12 aa shorter than that of isoform A (369 aa, reported as 93 kDa). Both are expressed in brain, stomach, intestinal epithelia, pancreatic islets and kidney tubules. Isoform B is also expressed in parotid, thyroid and bronchial glands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ICC, IF, IHC, IHC-P, WB
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Publications for Somatostatin R2/SSTR2 Antibody (MAB4224)(14)

We have publications tested in 2 confirmed species: Human, Primate.

We have publications tested in 4 applications: Flow, Flow Cytometry, ICC, Western Blot.


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Flow
(1)
Flow Cytometry
(1)
ICC
(2)
Western Blot
(1)
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Human
(5)
Primate
(1)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using MAB4224 Applications Species
HS Carr, Y Zuo, JA Frost The Wnt pathway protein Dvl1 targets Somatostatin receptor 2 for lysosome-dependent degradation The Journal of Biological Chemistry, 2023-03-23;0(0):104645. 2023-03-23 [PMID: 36965619] (Western Blot, Human) Western Blot Human
Mandriani B, Pellè E, Mannavola F et al. Development of anti-somatostatin receptors CAR T cells for treatment of neuroendocrine tumors Journal for immunotherapy of cancer 2022-06-01 [PMID: 35764366]
M Simón, JT Jørgensen, HA Khare, C Christense, CH Nielsen, A Kjaer Combination of [177Lu]Lu-DOTA-TATE Targeted Radionuclide Therapy and Photothermal Therapy as a Promising Approach for Cancer Treatment: In Vivo Studies in a Human Xenograft Mouse Model Pharmaceutics, 2022-06-16;14(6):. 2022-06-16 [PMID: 35745856] (Flow Cytometry, Human) Flow Cytometry Human
Bianca M. Dijkstra, Marion de Jong, Marcus C. M. Stroet, Fritz Andreae, Sebastiaan E. Dulfer, Marieke Everts et al. Evaluation of Ac-Lys0(IRDye800CW)Tyr3-octreotate as a novel tracer for SSTR2-targeted molecular fluorescence guided surgery in meningioma Journal of Neuro-Oncology 2021-06-01 [PMID: 33768405]
RM Agoglia, D Sun, F Birey, SJ Yoon, Y Miura, K Sabatini, SP Pa?ca, HB Fraser Primate cell fusion disentangles gene regulatory divergence in neurodevelopment Nature, 2021-03-17;0(0):. 2021-03-17 [PMID: 33731928] (ICC, Human, Primate) ICC Human, Primate
Shilpa Thakur, Brianna Daley, Corina Millo, Craig Cochran, Orit Jacobson, Huiyan Lu et al. 177Lu-DOTA-EB-TATE, a Radiolabeled Analogue of Somatostatin Receptor Type 2, for the Imaging and Treatment of Thyroid Cancer Clinical Cancer Research 2021-03-01 [PMID: 33355247]
Rachael Guenter, Tolulope Aweda, Danilea M. Carmona Matos, Samuel Jang, Jason Whitt, Yi-Qiang Cheng et al. Overexpression of somatostatin receptor type 2 in neuroendocrine tumors for improved Ga68-DOTATATE imaging and treatment Surgery 2020-01-01 [PMID: 31629542]
Rachael E. Guenter, Tolulope Aweda, Danilea M. Carmona Matos, Jason Whitt, Alexander W. Chang, Eric Y. Cheng et al. Pulmonary Carcinoid Surface Receptor Modulation Using Histone Deacetylase Inhibitors Cancers (Basel) 2019-06-03 [PMID: 31163616]
Guenter RE, Aweda T, Carmona Matos DM et al. Pulmonary Carcinoid Surface Receptor Modulation Using Histone Deacetylase Inhibitors Cancers (Basel) Jun 3 2019 [PMID: 31163616] (Flow, Human) Flow Human
Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al. Development and application of a high-content virion display human GPCR array Nature Communications 2019-04-30 [PMID: 31040288]
Show All 14 Publications.

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Bioinformatics

Gene Symbol SSTR2
Uniprot