Slit3 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Slit3 is a member of the slit family of large secreted axon guidance molecules that are ligands for Robo receptors (1). Like other mammalian family members, the 1523 amino acid (aa) Slit3 contains a signal sequence followed by 23 leucine-rich repeats (LRR) and nine EGF-like sequences (1). Mammalian Slits also contain a laminin-G domain between EGF6 and EGF7, and a C-terminal cysteine-rich domain (1). In Drosophila Slit, specific LRR are sites of ROBO interaction and homodimerization (2). Drosophila Slit shows equal similarities with all three mouse slit proteins, which are 59‑66% identical with each other (1). During development, Slit3 is expressed in the ventral neural tube, developing sensory organs, limb buds and developing areas of the limbs in patterns that overlap with but are discrete from Slit1 and Slit2 (1). Axons will not be allowed to recross the floor plate unless all three Slit genes are disrupted, suggesting some overlap in function (3). Slit3 is also expressed in the lung, kidney, skeletal muscle and heart, both during development and postnatally (1, 4‑6). ROBO2 is often found in complementary areas (4). Mice with genetically disrupted Slit3 have thin diaphragms with disorganized collagen fibrils and frequently develop diaphragmatic hernias (5, 6). Abnormalities in kidney development may also occur (5). Although Slit proteins are generally considered to be secreted, significant amounts of Slit3 may be retained in the mitochondria because of features in the signal sequence that indicate a high probability of mitochondrial targeting (7). Secreted Slit is often membrane-associated (7). Mouse Slit3 shows 98% and 94% amino acid identity with rat and human Slit3, respectively.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 3 applications: Immunohistochemistry, Neutralization, Western Blot.

Showing Publications 1 - 6 of 6.

Publications using AF3629	Applications	Species
Crane, J;Zhang, W;Otte, A;Barik, S;Wan, M;Cao, X; Slit3 by PTH-Induced Osteoblast Secretion Repels Sensory Innervation in Spine Porous Endplates to Relieve Low Back Pain Research square 2024-08-31 [PMID: 39257984] (Immunohistochemistry, Western Blot, Neutralization, Transgenic Mouse)	Immunohistochemistry, Western Blot, Neutralization	Transgenic Mouse
Hoving, JJA;Harford-Wright, E;Wingfield-Digby, P;Cattin, AL;Campana, M;Power, A;Morgan, T;Torchiaro, E;Quereda, V;Lloyd, AC; N-cadherin directs the collective Schwann cell migration required for nerve regeneration through Slit2/3-mediated contact inhibition of locomotion eLife 2024-04-09 [PMID: 38591541] (Western Blot, Mouse)	Western Blot	Mouse
K Jabbari, Q Cheng, G Winkelmaie, S Furuta, B Parvin CD36+ Fibroblasts Secrete Protein Ligands That Growth-Suppress Triple-Negative Breast Cancer Cells While Elevating Adipogenic Markers for a Model of Cancer-Associated Fibroblast International Journal of Molecular Sciences, 2022-10-22;23(21):. 10/22/2022 [PMID: 36361532]
Jabbari K, Winkelmaier G, Andersen C et al. Protein Ligands in the Secretome of CD36+ Fibroblasts Induce Growth Suppression in a Subset of Breast Cancer Cell Lines Cancers 9/8/2021 [PMID: 34572749]
Shuyun Wang, Shan Huang, Sean Johnson, Vadim Rosin, Jeffrey Lee, Eric Colomb et al. Tissue-specific angiogenic and invasive properties of human neonatal thymus and bone MSCs: Role of SLIT3-ROBO1 Stem Cells Translational Medicine 2020-09-01 [PMID: 32470195]
Greaves E, Collins F, Esnal-Zufiaurre A, Giakoumelou S, Horne A, Saunders P Estrogen receptor (ER) agonists differentially regulate neuroangiogenesis in peritoneal endometriosis via the repellent factor SLIT3. Endocrinology, 2014-07-22;155(10):4015-26. 2014-07-22 [PMID: 25051436] (Neutralization, Mouse)	Neutralization	Mouse

Array AF3629

• Slit3 Antibodies
• Slit3 ELISA Kits
• Slit3 Proteins and Enzymes