Home » Sirtuin 5/SIRT5 » Sirtuin 5/SIRT5 Proteins » Recombinant Human Sirtuin 5/SIRT5 His Protein
Novus Biologicals products are now on bio-techne.com

Recombinant Human Sirtuin 5/SIRT5 His Protein

 Submit a Review

Images

 
(
Enlarge)
SDS-Page: SIRT5 Protein [NBP2-22807]
SDS-Page: SIRT5 Protein [NBP2-22807]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human Sirtuin 5/SIRT5 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 34-310 of Human Sirtuin 5/SIRT5

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSARPSSSM ADFRKFFAKA KHIVIISGAG VSAESGVPTF RGAGGYWRKW QAQDLATPLA FAHNPSRVWE FYHYRREVMG SKEPNAGHRA IAECETRLGK QGRRVVVITQ NIDELHRKAG TKNLLEIHGS LFKTRCTSCG VVAENYKSPI CPALSGKGAP EPGTQDASIP VEKLPRCEEA GCGGLLRPHV VWFGENLDPA ILEEVDRELA HCDLCLVVGT SSVVYPAAMF APQVAARGVP VAEFNTETTP ATNRFRFHFQ GPCGTTLPEA LACHENETVS
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SIRT5
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
32.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 30% glycerol, 1 mM DTT
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Sirtuin 5/SIRT5 His Protein

  • EC 3.5.1
  • EC 3.5.1.-
  • FLJ36950
  • NAD-dependent deacetylase sirtuin-5
  • silent mating type information regulation 2, S.cerevisiae, homolog 5
  • SIR2L5
  • sir2-like 5
  • SIR2-like protein 5
  • SIRT5
  • sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
  • sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 5
  • Sirtuin 5
  • sirtuin type 5

Background

SIRT5 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants Recombinant human SIRT5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Sirtuin 3/SIRT3
Antibody (6B2A1) - BS ...
NBP2-52563
Immunohistochemistry Whole-Mount Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free
Flow Cytometry Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free
Western Blot Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free
(1 Review)
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC, ICC/IF, IHC-WhMt, IHC, IHC-P, WB
Sirtuin 1/SIRT1
Antibody (1F3) - BSA ...
NBP1-51641
Immunohistochemistry-Paraffin Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free
Flow Cytometry Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free
Immunocytochemistry/ Immunofluorescence Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free
(9 Publications)
Species: Hu, Mu, Pm, Rb, Rt
Applications: ELISA, Flow-IC, Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, WB
Sir2.1 Antibody
NB100-1923
Western Blot Sir2.1 Antibody
(1 Review)
(2 Publications)
Species: Ce
Applications: IHC, IHC-P, IP, WB
SIRT4 Antibody
NB100-1406
Western Blot SIRT4 Antibody
Immunohistochemistry-Paraffin SIRT4 Antibody
Western Blot SIRT4 Antibody
(5 Publications)
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
SIRT6 Antibody
NB100-2524
Western Blot SIRT6 Antibody
Immunocytochemistry/ Immunofluorescence SIRT6 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, WB
SIRT7 Antibody
NB100-56364
Western Blot SIRT7 Antibody
Western Blot SIRT7 Antibody
(1 Publication)
Species: Hu
Applications: WB
HDAC9 Antibody -
BSA Free
NBP2-03993
Western Blot HDAC9 Antibody - BSA Free
(1 Review)
(1 Publication)
Species: Bv, Ca, Hu, Mu, Pm
Applications: WB
Park7/DJ-1 Antibody
NB300-270
Immunohistochemistry-Paraffin Park7/DJ-1 Antibody
Western Blot Park7/DJ-1 Antibody
Western Blot Park7/DJ-1 Antibody
(3 Reviews)
(23 Publications)
Species: Ch, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, In vitro, Simple Western, WB
CPS1 Antibody
NBP1-86019
Immunohistochemistry-Paraffin CPS1 Antibody
Immunohistochemistry-Paraffin CPS1 Antibody
Immunohistochemistry-Paraffin CPS1 Antibody
(1 Publication)
Species: Ca, Hu
Applications: ICC/IF, IHC, IHC-P, WB
CYP21A2 Antibody
NBP2-13893
Immunohistochemistry-Paraffin CYP21A2 Antibody
Immunohistochemistry-Paraffin CYP21A2 Antibody
Immunohistochemistry-Paraffin CYP21A2 Antibody
Species: Hu
Applications: IHC, IHC-P, WB
Recombinant Human
PM20D1 GST (N-Term) P ...
H00148811-P01
SDS-PAGE Recombinant Human PM20D1 GST (N-Term) Protein
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
Peroxiredoxin 2
Antibody (4E10-2D2)
H00007001-M01
ELISA Peroxiredoxin 2 Antibody (4E10-2D2)
Knockdown Validated Peroxiredoxin 2 Antibody (4E10-2D2)
Western Blot Peroxiredoxin 2 Antibody (4E10-2D2)
(1 Review)
(6 Publications)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
Recombinant Human
HIST1H4I GST (N-Term) ...
H00008294-P01
SDS-PAGE Recombinant Human HIST1H4I GST (N-Term) Protein
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
Acetyl CoA
synthetase Antibody
NBP1-90276
Immunohistochemistry-Paraffin Acetyl CoA synthetase Antibody
Immunohistochemistry-Paraffin Acetyl CoA synthetase Antibody
Immunohistochemistry-Paraffin Acetyl CoA synthetase Antibody
(2 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
UCP1 Antibody
(536435) [Unconjugate
MAB6158
Simple Western UCP1 Antibody (536435) [Unconjugated]
Immunocytochemistry UCP1 Antibody (536435) [Unconjugated]
Intracellular Staining by Flow Cytometry UCP1 Antibody (536435) [Unconjugated]
(51 Publications)
Species: Hu, Mu
Applications: ICC, ICFlow, Simple Western, WB
UCP5 Antibody
NBP1-59598
Western Blot UCP5 Antibody
(3 Publications)
Species: Hu, Mu
Applications: ICC/IF, WB
PGC1 alpha
Antibody - BSA Free
NBP1-04676
Western Blot PGC1 alpha Antibody - BSA Free
Immunohistochemistry-Paraffin PGC1 alpha Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence PGC1 alpha Antibody - BSA Free
(11 Reviews)
(278 Publications)
Species: Gt, Ha, Hu, Mu, Po, Rt, Sh, Sq
Applications: ChIP, ChIP, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Histone H4 Antibody
AF5215
Western Blot Histone H4 Antibody
Immunocytochemistry Histone H4 Antibody
(6 Publications)
Species: Hu, Mu
Applications: ICC, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow

Publications for Sirtuin 5/SIRT5 Protein (NBP2-22807) (0)

There are no publications for Sirtuin 5/SIRT5 Protein (NBP2-22807).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Sirtuin 5/SIRT5 Protein (NBP2-22807) (0)

There are no reviews for Sirtuin 5/SIRT5 Protein (NBP2-22807). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Sirtuin 5/SIRT5 Protein (NBP2-22807) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Sirtuin 5/SIRT5 Products

Research Areas for Sirtuin 5/SIRT5 Protein (NBP2-22807)

Find related products by research area.

Blogs on Sirtuin 5/SIRT5

There are no specific blogs for Sirtuin 5/SIRT5, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Vehicle or RU 486 (5 mg/kg) injected mice were presented with/without corticosterone (10 mg/kg) for 3 days. The expressions of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha NBP1-04676) and NIX (BNIP3L NBP1-88558) were visualized via western blotting. Loading control is beta-actin. n = 5. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105/) licensed under a CC-BY license.
PGC1 alpha Antibody - BSA Free
NBP1-04676
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
p53 Antibody (PAb 240) - BSA Free
NB200-103

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Sirtuin 5/SIRT5 His Protein and receive a gift card or discount.


Bioinformatics

Gene Symbol SIRT5