Home » Siglec-3/CD33 » Siglec-3/CD33 Antibodies » Siglec-3/CD33 Antibody (44M12D3) [CoraFluor™ 1]
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Siglec-3/CD33 Antibody (44M12D3) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
44M12D3
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22377AF350
Alexa Fluor 405
NBP2-22377AF405
Alexa Fluor 488
NBP2-22377AF488
Alexa Fluor 532
NBP2-22377AF532
Alexa Fluor 594
NBP2-22377AF594
Alexa Fluor 647
NBP2-22377AF647
Alexa Fluor 700
NBP2-22377AF700
Alexa Fluor 750
NBP2-22377AF750
Biotin
NBP2-22377B
DyLight 350
NBP2-22377UV
DyLight 405
NBP2-22377V
DyLight 488
NBP2-22377G
DyLight 550
NBP2-22377R
DyLight 594
NBP2-22377DL594
DyLight 650
NBP2-22377C
DyLight 680
NBP2-22377FR
DyLight 755
NBP2-22377IR
FITC
NBP2-22377F
HRP
NBP2-22377H
Janelia Fluor 525
NBP2-22377JF525
Janelia Fluor 549
NBP2-22377JF549
Janelia Fluor 646
NBP2-22377JF646
Janelia Fluor 669
NBP2-22377JF669
Unconjugated
NBP2-22377
mFluor Violet 450 SE
NBP2-22377MFV450
mFluor Violet 500 SE
NBP2-22377MFV500
mFluor Violet 610 SE
NBP2-22377MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-22377
BSA Free
NBP2-22377
BSA Free
NBP2-22377SS
Azide and BSA Free
NBP2-80948
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Siglec-3/CD33 Antibody (44M12D3) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Amino acids 42-159 of human CD33 were used as the immunogen for the antibody.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD33
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Siglec-3/CD33 Antibody (44M12D3) [CoraFluor™ 1]

  • CD33 antigen (gp67)
  • CD33 antigen
  • CD33 molecule
  • CD33
  • FLJ00391
  • gp67
  • myeloid cell surface antigen CD33
  • p67
  • sialic acid binding Ig-like lectin 3
  • Sialic acid-binding Ig-like lectin 3
  • Siglec3
  • Siglec-3
  • SIGLEC3gp67

Background

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Siglec-3/CD33 Antibody (44M12D3) [CoraFluor™ 1]
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Bioinformatics

Gene Symbol CD33