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SFPQ Antibody (6D7)

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Western Blot: SFPQ Antibody (6D7) [H00006421-M02] - SFPQ monoclonal antibody (M02), clone 6D7 Analysis of SFPQ expression in Hela S3 NE.
Western Blot: SFPQ Antibody (6D7) [H00006421-M02] - SFPQ monoclonal antibody (M02), clone 6D7 Analysis of SFPQ expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: SFPQ Antibody (6D7) [H00006421-M02] - Analysis of monoclonal antibody to SFPQ on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SFPQ Antibody (6D7) [H00006421-M02] - Analysis of monoclonal antibody to SFPQ on formalin-fixed paraffin-embedded human thyroid nodular goiter. Antibody concentration 3 ug/ml.
ELISA: SFPQ Antibody (6D7) [H00006421-M02] - Detection limit for recombinant GST tagged SFPQ is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
6D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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SFPQ Antibody (6D7) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
SFPQ (NP_005057, 269 a.a. ~ 361 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQ
Localization
Nuclear
Specificity
SFPQ - splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SFPQ
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Publications
Read Publications using H00006421-M02.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SFPQ Antibody (6D7)

  • 100 kDa DNA-pairing protein
  • DNA-binding p52/p100 complex, 100 kDa subunit
  • hPOMp100
  • polypyrimidine tract binding protein associated
  • polypyrimidine tract-binding protein-associated splicing factor
  • Polypyrimidine tract-binding protein-associated-splicing factor
  • PSFPOMP100
  • PTB-associated splicing factor
  • PTB-associated-splicing factor
  • splicing factor proline/glutamine rich (polypyrimidine tract binding proteinassociated)
  • splicing factor proline/glutamine rich (polypyrimidine tract-bindingprotein-associated)
  • splicing factor proline/glutamine-rich
  • splicing factor, proline- and glutamine-rich

Background

Essential pre-mRNA splicing factor required early in spliceosome formation. Binds to the mammalian polypyrimidine tracts. Forms a complex with the polypyrimidine tract-binding protein (PTB). Seems to also bind DNA. It is a heterotetramer of two 52 kDa and two 100 kDa subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SFPQ Antibody (H00006421-M02)(3)


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Bioinformatics

Gene Symbol SFPQ
Entrez
OMIM
Uniprot